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In Category

We narrowed to 711 results for: tat

Showing: 441 - 460 of 711 results

  1. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...the protein folded, changing it from its native state to a less folded, or denatured, one. This enables... protein of interest while it’s in a transition state or specific configuration. So how do you ensure ...

  4. Plasmids 101: TOPO Cloning

    Type
    Blog Post
    Published
    Oct. 27, 2016, 2:30 p.m.
    ...cleaving and rejoining supercoiled DNA ends to facilitate replication. The TOPO technique specifically ...Site Directed Mutagenesis by PCR Use REPLACR Mutatgenesis to Easily Generate Insertion and Deletions in...

  5. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    Published
    Oct. 29, 2015, 2:30 p.m.
    ... the National Academy of Sciences of the United States of America 110.39 (2013): 15521–15529. PMC. PubMed... the National Academy of Sciences of the United States of America 70.11 (1973): 3240–3244. PubMed PMID...

  6. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...requires very little hands-on labor and could facilitate the tagging of every protein in the C. elegans...other genome modifications, including targeted mutations. SEC-based systems may prove effective in other...

  8. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    Published
    May 21, 2019, 12:10 p.m.
    ...cellular components ultimately leading to cell fragmentation and death (Elmore, 2007). To study apoptosis...fluorescence based reporters however have several limitations. First, FRET signals are often difficult to detect...

  9. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...under CC BY from Mattei et.al., 2022.   Limitations of the in silico approach The in silico approach... verification. All approaches have their own limitations. Using methods together can inform decisions ...

  10. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...repository provides. Repositories around the world facilitate the rapid pace of SARS-CoV-2 research by removing...and SARS-CoV with implications for clinical manifestations, transmissibility, and laboratory studies of...

  11. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ...makes it easier to spot even minor weakness or limitations in a piece of research (Drummond & Fischhoff,... the National Academy of Sciences of the United States of America, 114(36), 9587-9592. doi:10.1073/pnas...

  13. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...However, the first two generations of iGluSnFR had limitations: they were not optimal for experiments requiring...photon imaging.   Figure 1: 1P and 2P excitation and emission spectra of SF-Venus-iGluSnFR.A184V...

  14. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection. Expression is driven...QBI UCSF. They are particularly interested in facilitating collaborative science, and making it accessible...

  16. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...translated in a specific compartment. Despite these limitations, these techniques represent powerful tools for...translation in various cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide...

  18. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...below. Normalization is a fundamental and often understated problem in multiplex NGS library preparation....seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for solving critical...

  19. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ... the purpose of the meeting: Begin meetings by stating “The purpose of this meeting is to _________." ...ambassador” from each affected team to be the representative in the meeting and then communicate outcomes...

  20. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter jejuni (CJ) 3' NNNNRYAC  Neisseria... as a scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived...
Showing: 441 - 460 of 711 results