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We narrowed to 694 results for: ust

Showing: 441 - 460 of 694 results

  1. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...stabilize the 3’ end of the pegRNA. As research has just begun with PE7, it will be exciting to see future... target cells (Figure 6).   Figure 6: Illustration of split fluorophore assay to test reconstitution...

  2. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...drug resistance status, it is a very common, well-trusted method that is frequently used in the clinical ...final report and recommendations." London: Wellcome Trust & HM Government (2016). 2. CDC Information Pages...

  3. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...today (Fagone et al., 2012). PCR-based methods are robust, easy, fast, and convenient. However, these methods... starting material in the PCR because the sample must fall within the linear region of the standard curve...

  4. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...plasmids used in the validation assay (above) and it is just as effective (Mashiko et al., 2014). The plasmid...needed when dealing with RNA (e.g. face mask, and a dust free and specially cleaned work bench). Once the...

  5. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...By Justin Ng and Alyssa Cecchetelli Science is a constantly evolving and demanding field, requiring a...experiments! References: Blount, Z. D. (2015). The unexhausted potential of E. coli. ELife, 4. https://doi.org...

  6. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...video from iBiology). IHC and ICC are essentially just more general terms that can also refer to assays...fluorophores. It is common for labs to keep a few trusted secondary antibodies on hand to mix and match with...

  8. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...been following Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you should be...(Alam et al., 2017). Yellow fluorescent aptamers Just as vegetables come in a variety of colors, so do...

  9. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...plants, most bacteria and some fungi, thus mammals must obtain the vitamin through external sources. Humans...biotin and avidin, macromolecules of interest first must be biotinylated. Scientists have developed several...

  10. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...complementary to their target sequence. However, you can’t just use the mature sequence in a plasmid and call it...common issue with many gene silencing methods and just needs to be taken into account when designing experiments...

  11. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...candidate in Neuroscience at Harvard University. Just as computers, cell phones, and cars become more ...throughout the amplification process, rather than just at the end as in standard PCR. This allows the researcher...

  12. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...sites (like, indeed.com, or salary.com). Search industry and academic job titles in the regions where the...to proceed with a used or new item. To further illustrate this point, if your research is dependent on ...

  14. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...few gRNAs to choose from that sequence preferences must largely be ignored. Created with BioRender.com... For C>T and A>G base editors, the intended edit must be in a 5 - 10 nt window relative to the PAM, and...

  15. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...tools but their use is limited in vivo because they must be excited by external light sources. These external... subcellular structures and gene expression. You must see the movies demonstrating Ca2+ dynamics in cardiomyocytes...

  16. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...it off, allowing the cell to be excited over a sustained period of time (Gong et al., 2020). Light-gated...Bendifallah, I., Zhou, F., Zeitzschel, N., Ahlbeck, J., Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk...

  17. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...downright infuriating at worst. While it is tempting to just dive right in and start injecting your virus, a ...through the endogenous GFAP promoter results in robust transduction in the rat spinal cord following injury...

  18. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...genetic tools that consist of two parts, both of which must be present for a gene of interest to be expressed...Liqun Luo’s lab developed the Q system from a gene cluster in the fungus Neurospora crassa. The QUAS promoter...

  19. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...special: Strong expression: A CMV promoter drives robust gene expression in mammalian cells. Flexible tagging... Antibody Data Hub. Perhaps these antibodies are just the tools you need to push the field forward! Browse...

  20. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...study. These are highly useful for the biotech industry, which is in the process of capitalizing on phage...easily varied by the cell and whose structures are just beginning to be understood. Therefore, in addition...
Showing: 441 - 460 of 694 results