We narrowed to 707 results for: ust

... with adeno-associated viruses (AAVs), will know just how tricky these tools can be to use. AAVs, which... from our plasmid lab to develop a reliable and robust high quality viral vector service, making an array...

... the detection antibody and the capture antibody must bind to distinct, non-overlapping epitopes so that...development, however, can be challenging since scientists must first identify two independent antibodies that work...

...stabilize the 3’ end of the pegRNA. As research has just begun with PE7, it will be exciting to see future... target cells (Figure 6).   Figure 6: Illustration of split fluorophore assay to test reconstitution...

...rest of the genome and (2) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence... SpCas9, indicating that the variants are likely just as selective as wild-type SpCas9. These VQR, EQR...

...today (Fagone et al., 2012). PCR-based methods are robust, easy, fast, and convenient. However, these methods... starting material in the PCR because the sample must fall within the linear region of the standard curve...

...surprising for Addgene. But the names we chose were not just any gene names. Now, we have meetings in rooms such...activate bagpipe which is localized to segmental clusters of the mesoderm. This subdivision of the mesoderm...

...coding sequence of interest (CDS1, top). Overhangs must match the Level 0 acceptor (bottom) for CDS1 parts...the learning and planning, and your MoClo kit has just arrived! MoClo kits from Addgene will most likely...

...regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten base pairs and...Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias...

...drug resistance status, it is a very common, well-trusted method that is frequently used in the clinical ...final report and recommendations." London: Wellcome Trust & HM Government (2016). 2. CDC Information Pages...

...plasmids used in the validation assay (above) and it is just as effective (Mashiko et al., 2014). The plasmid...needed when dealing with RNA (e.g. face mask, and a dust free and specially cleaned work bench). Once the...

...video from iBiology). IHC and ICC are essentially just more general terms that can also refer to assays...fluorophores. It is common for labs to keep a few trusted secondary antibodies on hand to mix and match with...

... difficult to predict which one will work as illustrated in figure 3 (Mastop et al., 2018). Unbiased, ...Jakobus, et al. "A New generation of FRET sensors for robust measurement of Gαi1, Gαi2 and Gαi3 activation kinetics...

...been following Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you should be...(Alam et al., 2017). Yellow fluorescent aptamers Just as vegetables come in a variety of colors, so do...

...plants, most bacteria and some fungi, thus mammals must obtain the vitamin through external sources. Humans...biotin and avidin, macromolecules of interest first must be biotinylated. Scientists have developed several...

...complementary to their target sequence. However, you can’t just use the mature sequence in a plasmid and call it...common issue with many gene silencing methods and just needs to be taken into account when designing experiments...

...By Justin Ng and Alyssa Cecchetelli Science is a constantly evolving and demanding field, requiring a...experiments! References: Blount, Z. D. (2015). The unexhausted potential of E. coli. ELife, 4. https://doi.org...

...candidate in Neuroscience at Harvard University. Just as computers, cell phones, and cars become more ...throughout the amplification process, rather than just at the end as in standard PCR. This allows the researcher...

...sites (like, indeed.com, or salary.com). Search industry and academic job titles in the regions where the...to proceed with a used or new item. To further illustrate this point, if your research is dependent on ...

... as tightly packed and interwoven as the brain, robust single-cell labeling is necessary to distinguish...anemone mKate2.) They then successfully generated custom antibodies to each of these proteins and confirmed...

...it off, allowing the cell to be excited over a sustained period of time (Gong et al., 2020). Light-gated...Bendifallah, I., Zhou, F., Zeitzschel, N., Ahlbeck, J., Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk...