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Showing: 441 - 460 of 707 results
  1. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... with adeno-associated viruses (AAVs), will know just how tricky these tools can be to use. AAVs, which... from our plasmid lab to develop a reliable and robust high quality viral vector service, making an array...
  2. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ... the detection antibody and the capture antibody must bind to distinct, non-overlapping epitopes so that...development, however, can be challenging since scientists must first identify two independent antibodies that work...
  3. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...stabilize the 3’ end of the pegRNA. As research has just begun with PE7, it will be exciting to see future... target cells (Figure 6).   Figure 6: Illustration of split fluorophore assay to test reconstitution...
  4. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...rest of the genome and (2) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence... SpCas9, indicating that the variants are likely just as selective as wild-type SpCas9. These VQR, EQR...
  5. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...today (Fagone et al., 2012). PCR-based methods are robust, easy, fast, and convenient. However, these methods... starting material in the PCR because the sample must fall within the linear region of the standard curve...
  6. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...coding sequence of interest (CDS1, top). Overhangs must match the Level 0 acceptor (bottom) for CDS1 parts...the learning and planning, and your MoClo kit has just arrived! MoClo kits from Addgene will most likely...
  7. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten base pairs and...Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias...
  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...drug resistance status, it is a very common, well-trusted method that is frequently used in the clinical ...final report and recommendations." London: Wellcome Trust & HM Government (2016). 2. CDC Information Pages...
  9. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...plasmids used in the validation assay (above) and it is just as effective (Mashiko et al., 2014). The plasmid...needed when dealing with RNA (e.g. face mask, and a dust free and specially cleaned work bench). Once the...
  10. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...video from iBiology). IHC and ICC are essentially just more general terms that can also refer to assays...fluorophores. It is common for labs to keep a few trusted secondary antibodies on hand to mix and match with...
  11. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...been following Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you should be...(Alam et al., 2017). Yellow fluorescent aptamers Just as vegetables come in a variety of colors, so do...
  12. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...plants, most bacteria and some fungi, thus mammals must obtain the vitamin through external sources. Humans...biotin and avidin, macromolecules of interest first must be biotinylated. Scientists have developed several...
  13. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...complementary to their target sequence. However, you can’t just use the mature sequence in a plasmid and call it...common issue with many gene silencing methods and just needs to be taken into account when designing experiments...
  14. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...By Justin Ng and Alyssa Cecchetelli Science is a constantly evolving and demanding field, requiring a...experiments! References: Blount, Z. D. (2015). The unexhausted potential of E. coli. ELife, 4. https://doi.org...
  15. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...candidate in Neuroscience at Harvard University. Just as computers, cell phones, and cars become more ...throughout the amplification process, rather than just at the end as in standard PCR. This allows the researcher...
  16. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...sites (like, indeed.com, or salary.com). Search industry and academic job titles in the regions where the...to proceed with a used or new item. To further illustrate this point, if your research is dependent on ...
  17. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...it off, allowing the cell to be excited over a sustained period of time (Gong et al., 2020). Light-gated...Bendifallah, I., Zhou, F., Zeitzschel, N., Ahlbeck, J., Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk...
Showing: 441 - 460 of 707 results