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Showing: 441 - 460 of 707 results
  1. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...control by Addgene before being distributed to customers. Details about our production protocols, titering...Addgenies and most recently updated by Ina Ersing in August 2023. Resources and references Resources at the...
  2. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...editing treatment would have to be individually customized to the patient's specific mutations. With a drag... Yarnall, Cian Schmitt-Ulms, Rohan N. Krajeski, Justin Lim, Lukas Villiger, Wenyuan Zhou, Kaiyi Jiang,...
  3. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...place people share ideas. Ideas had over lunch are just as valuable! In 2019, Addgene implemented a company-wide... idea into a viable solution.  Anticipate Change Just because a process is working, doesn’t mean it will...
  4. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...affect binding the lysis buffer may need to be adjusted depending on the sample. It is important to remember...make this approach appealing, but the disadvantages must be carefully considered before using. For example...
  5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...rest of the genome and (2) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence... SpCas9, indicating that the variants are likely just as selective as wild-type SpCas9. These VQR, EQR...
  6. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...today (Fagone et al., 2012). PCR-based methods are robust, easy, fast, and convenient. However, these methods... starting material in the PCR because the sample must fall within the linear region of the standard curve...
  7. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten base pairs and...Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias...
  8. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...drug resistance status, it is a very common, well-trusted method that is frequently used in the clinical ...final report and recommendations." London: Wellcome Trust & HM Government (2016). 2. CDC Information Pages...
  9. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    Published
    June 28, 2016, 10:59 a.m.
    ...plasmids used in the validation assay (above) and it is just as effective (Mashiko et al., 2014). The plasmid...needed when dealing with RNA (e.g. face mask, and a dust free and specially cleaned work bench). Once the...
  10. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...complementary to their target sequence. However, you can’t just use the mature sequence in a plasmid and call it...common issue with many gene silencing methods and just needs to be taken into account when designing experiments...
  11. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...video from iBiology). IHC and ICC are essentially just more general terms that can also refer to assays...fluorophores. It is common for labs to keep a few trusted secondary antibodies on hand to mix and match with...
  12. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...By Justin Ng and Alyssa Cecchetelli Science is a constantly evolving and demanding field, requiring a...experiments! References: Blount, Z. D. (2015). The unexhausted potential of E. coli. ELife, 4. https://doi.org...
  13. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...been following Addgene on #souptwitter or if you just enjoy a warm meal on a winter day, you should be...(Alam et al., 2017). Yellow fluorescent aptamers Just as vegetables come in a variety of colors, so do...
  14. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...plants, most bacteria and some fungi, thus mammals must obtain the vitamin through external sources. Humans...biotin and avidin, macromolecules of interest first must be biotinylated. Scientists have developed several...
  15. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...candidate in Neuroscience at Harvard University. Just as computers, cell phones, and cars become more ...throughout the amplification process, rather than just at the end as in standard PCR. This allows the researcher...
  16. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...sites (like, indeed.com, or salary.com). Search industry and academic job titles in the regions where the...to proceed with a used or new item. To further illustrate this point, if your research is dependent on ...
  17. Hot Plasmids: Fall 2025

    Type
    Blog Post
    Published
    Nov. 11, 2025, 2:14 p.m.
    ...metastatic capacity of cancer cells. This is certainly just the beginning for MitoTRACER, as it is a diverse...advantages. While most red GECIs have been derived from just a few parent fluorescent proteins, PinkyCaMP is ...
Showing: 441 - 460 of 707 results