...binding affinity (Janda et al., 2016; Torres & Casadevall, 2008) but…does it really do anything? Fc functions...an antigen-bound antibody and induce (or, in the case of the Fc receptors FcγRIIB1 and FcγRIIB, inhibit...system's classical complement pathway, starting a cascade of events that results in the pores being opened....2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects on Variable...articles/10.3389/fmicb.2016.00022 Torres, M., & Casadevall, A. (2008). The immunoglobulin constant region...

...inactivates or completely removes (in the case of Gateway cloning) one cassette. Counterselection allows the scientist... Scientists introduce an antibiotic resistance cassette within the coding region of the gene they are ...is best practice not to use the same resistance cassette for the mutation and for plasmid selection. Additionally... vector typically has two separate resistance cassettes and your gene of interest is cloned into/inactivates...

... the flexibility needed for multiplexing. In the case above, you can’t costain with your desired antibodies...easily manipulated into more useful tools. In the case of isotype conversion, the variable regions of a... species and/or isotype (Liddell 2013). In most cases, the resulting chimeric antibody retains the binding...properties (Morelock 1994). While in the majority of cases the differences are subtle, chimeric antibodies ...when multiplexing with chimeric antibodies. In the case above you should use an anti-rat IgG1 secondary ...

...independence, such as museums, YouTube channels and podcasts, science pub nights, and pop sci books. Most scicomm... that is widely used but has a very specific use-case (definition) in a specific field.  For example, ...also students in AP biology classes Creating a podcast about genetics using dog breeds as an example ...interested in breeding dogs who also enjoy listening to podcasts A series of Tweets breaking down a new publication...others from accessing their work. But that’s not the case. In fact, it’s really the opposite. Unlike emails...

... carefully presented case for better more diverse science. It's a series of case studies in what happens... other works of Dr. Sacks, Musicophilia includes case studies of relevant neurological atypicalities, ...the Dark Lord rules all, the books center around a cast of endearing characters who decide to take matters...Ice Age to the 1980s, Sarum follows an intriguing cast of characters from five different families over ...

... PhD Podcast episode 15 "Simple tricks for time management: the pomodoro technique": This podcast talks... References and Resources You Can Grad School podcast episode 2 "Organizing Literature" : I found out...out about Zotero here Dear Grad Student podcast episode 2 "Essential Grad School Resources: Citation Managers...Managers, Programming, & Productivity": This podcast talks about Mendeley, Zotero, and other citation ...

... to help you find your desired plasmid. In many cases, you will need a screening strategy as well. In ...comes in. Let’s take a look at All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab..., without the need to prep your plasmid. In this case, you’d be using colony PCR to detect your insert...the other primer anneals to the backbone. In this case, if there is no insert in the plasmid, you won’t...order of nucleotides within a DNA molecule, in this case a plasmid. Sequencing is one of the surest ways ...

...selective degradation of FKBP12F36V-BRD4 using CRISPR/Cas9-mediated knock-in, and to evaluate in vivo degradation... and endogenous ENL was inactivated using CRISPR/Cas9 gene-editing. Featured molecules: dTAG-7 and dTAG...and endogenous MELK was inactivated using CRISPR/Cas9 gene-editing. Featured molecules: dTAG-7, dTAG-13...engineered to express YY1-FKBP12F36V using CRISPR/Cas9-mediated knock-in. Featured molecule: dTAG-47 Additional...Knock-In and Conditional Mouse Models CRISPR 101: Cas9 Nickase Design and Homology Directed Repair Plasmids...

...progestin hormone therapy resulted in 76,000 fewer cases of cardiovascular disease, 126,000 fewer breast ...methods of sex and gender analysis and 2) provides case studies with concrete examples of how gender analysis...analysis leads to discovery and innovation. One such case study looks at the effects of using all female cells...also consider the sex of the stem cells. In this case, they found that sex-matching of donor and recipient...analyze these factors during an experiment. In the case of stem cells, these factors may include cell type...

...activity and minimize off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180...transcriptional activation by an engineered CRISPR-Cas9 complex." Nature (2014). PubMed PMID: 25494202. ...PMC4559080. 8. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout screening in human cells." Science 343.6166..."Genetic screens in human cells using the CRISPR-Cas9 system." Science 343.6166 (2014): 80-84. PubMed ...Wong, Alan SL, et al. "Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM." Proceedings of the...