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We narrowed to 790 results for: Cre

Showing: 231 - 240 of 790 results

  1. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...CRISPR pooled gRNA libraries that can be used to screen for genes involved in a wide variety of cellular...processes. While these are widely requested and incredibly useful tools, they require extensive background... infect target cells, and, finally, conduct the screen. To help alleviate some of this labor for our ...optimization-scale infection and at least one screening-scale experiment achieving the gRNA representation...) says: "When you're setting up to do a genetic screen, you need to focus on building a really good model...use along with lentiviral packaging plasmids to create your own lentivirus stocks. Our research scientists...

  2. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    Published
    March 17, 2020, 1:32 p.m.
    ...David Liu’s lab created the first base editor in 2016 (Komor et al., 2016) and since then has been trying...David Liu’s lab set out to evolve ABEs with an increased compatibility to Cas homologs. Evolving better...bacteriophage, which rely on the expression of gene III to create infectious particles. A plasmid-based genetic circuit...III to base editor activity and editing speed. Created with BioRender.com. Introducing ABE8e - a fast...about 7 times higher than ABE7.10. However, the increased editing speed and targeting scope of ABE8e comes...comes with a price: ABE8e showed increased off-target DNA and off-target RNA activity. To mitigate this...

  3. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    Published
    Jan. 30, 2020, 2:15 p.m.
    ...psychological sciences were called into question after credible attempts to replicate major findings could not...our readers into believing the results are more credible than they actually are. Throwing out plates that...preregistration Preregistration is the process of creating a time-stamped research plan in a public repository...the Center for Open Science in order to reward increased transparency and reduced bias in scientific research...Openness Promotion Guidelines; preregistration to increase clarity in study design and analysis; removing...bias with Registered Reports; and recognizing increased transparency with badges. Before coming to the...

  4. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...targeted via RNAi. Recently the Vellai lab has created an improved RNAi vector that leads to significantly...non-specific nucleotides into the dsRNA leading to increased RNAi efficiency. Find the T444T plasmid here... used yeast display-based directed evolution to create APEX2. They have now applied the same directed ...the biotin ligase from which BioID was derived, creating a pair of enzymes that overcome both the speed...BioID, which only functions optimally at 37℃. The increased efficiency of TurboID and miniTurbo makes PL a...include protein stability, small molecule binding screens, peptide arrays, and phage display selections. ...

  5. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type
    Blog Post
    Published
    June 6, 2019, 12:41 p.m.
    ...CIB1 to regulate botulinum neurotoxin were also created. The depositors demonstrated the utility of light-mediated...these gas vesicle proteins scatter sound waves and create ultrasound contrast. At a very low volumetric density...s lab at MIT pushed the field further with the creation of the largest miRNA sensor collection reported...al., Nat Commun, 2019. PubMed PMID: 29934631. Screening immune cell lineages in vivo using the CRISPR-...tools have been used in immunology for in vitro screens of immune cell activity. However, a CRISPR-based...gene. CHIME can also be used for high throughput screens using pooled libraries. Find the CHIME plasmids...

  6. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...could mean needing to increase crosslinking to counteract the effects of increased sample agitation; a ...such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for exploring these interactions...crosslinking, which uses formaldehyde or UV light to create strong but reversible bonds. This will keep the... necessary for most ChIP experiences, but the increased binding can mask the epitopes antibodies recognize...shorter sonication time could be an opportunity to decrease crosslinking. (Homogenization for a tissue sample...

  7. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...recording data. Increasing the voltage will shift the population to the right while decreasing the voltage...clearly distinguishable as two separate populations. Created with biorender.com. Pro tip! If your samples...smeary dot plot shows the population of interest. Created with biorender.com.   In such cases, instead...histogram plot for a yes/no flow cytometry readout. Created with biorender.com.   Using histogram plots...baseline expression of the protein of interest. Created with biorender.com. The plot above is for a...

  8. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...assaying a secreted protein, first block the Golgi apparatus with Brefeldin A to inhibit secretion and then...through the cell membrane. Formaldehyde fixation creates cross-links between lysine residues thus preserving...flow cytometry data users will typically start by creating a dot plot of FSC versus SSC that allows them ...initially gating the lymphocytes, one could then create a dot plot of CD3 (T cell marker) versus CD19 (...cells, might then gate the B cell population and create a histogram of CD19-containing cells versus total...cells in that sample. Alternatively, they could create additional dot plots of various CD markers to further...

  9. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ... technology has been used to optimize protein secretion and expression in the biotech workhorse yeast ...Engineering disease resistance through gene stacking, increasing production of a protein of interest, and improving...genetic circuits with multiplexed guides, mini screening libraries, direct edit optimization, and more....can be invaluable for more complex editing and screening experiments in organisms that don’t yet have these...modify it as necessary. And remember, if you end up creating a really cool MoClo kit, we would love for you...Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol. 6, no.... of the GoldenBraid Modular Cloning System and Creation of a Toolkit for the Expression of Heterologous...

  10. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...may not result in any cells, or all the colonies screened might not contain the correct plasmid. There’s...and if the sequence has high GC or AT content, increase the overlapping region of the primer to 40-60 ...buffers used in the previous reactions. This will increase your chances to have a successful and ultimately...pick the big colony and you are safe! Colony screening Pick a few colonies (5-10) and grow them in a ... you've identified potential clones after the screening, sequence the plasmid. Many vectors contain sequences...can use the chromatogram to spot whether any discrepancies between your sequencing result and the expected...
Showing: 231 - 240 of 790 results