We narrowed to 803 results for: Cre

...International Mentorship Program (IMP) USA-Europe was created. IMP as an idea was conceived and developed by ...the specific technical and logistic support to increase the chances of success, but also nurture a supportive...country of origin with an international mentor creates a closer and more approachable relationship for...at IMP In these sessions, mentors helped mentees create “Career Development Plans” which provided paths...developmental needs and resources needed to pursue them, increased their network of contacts important for their ...Conference highlights included remarks by the Spanish Secretary of Research, Development, and Innovation, D. Carmen... as they witness their student’s progress and increases in self confidence. These improvements, combined...

...positive selection, negative selection, and/or screening. We’re focusing on positive and negative selection...in this blog post, but don’t worry, we’ll cover screens in a future post. Positive selection: Cells that...with or without your inserted DNA) and further screening will help you determine if the plasmid backbone...arises in yeast. For example, Markus Ralser’s lab created a yeast prototrophy kit containing 23 plasmids ...bacteria to grow on sucrose. Plasmid cloning: Creativity with naturally occurring genes  At this point... scientists are finding more and more ways to creatively perform selections in plasmid cloning. Additional...

...are under a Creative Commons 3.0 license, which requires users to give appropriate credit, provide a link...about new scientific techniques and cool plasmids. Creating graphics were usually the last thing I thought..., I found some free design software that let me create graphics that better communicated the science in...reasons why this was the first program I used to create graphics for the Addgene blog. However, it has ...requested, although there’s no guarantee Biorender will create it. Additionally, you can upload your own images...

... biosensor creation, and custom FRET biosensors can be created using the cpFRET kit created by Olivier...Robert Campbell’s lab display calcium-dependent increases in fluorescence of up to 11,000-fold. FRET biosensors...biosensors. Blue flame “cameleon” calcium sensors created by Roger Tsien’s lab contain two fluorescent proteins...calcium binds, it triggers CaM/M13 interaction that increases FRET transfer between the fluorescent proteins...

...experiments but we can definitely give you credit for it and increase your impact! Download ReFigure extension...along with the article metadata, legend and user created notes on a “Pinterest for scientists”? ReFigure...Linkedin and ResearchGate profile. To see how to create ReFigures in a few minutes, visit our YouTube [email protected] Please support ReFigure by creating ReFigures and answering this 2 minute survey! ...impact of teaching and doing science is rapid, concrete and open to innovation by all.  Additional Resources...

...membrane depolarization increases the BRET signal whereas hyperpolarization decreases the signal. The authors...technologies that can accelerate your research. The creation of NanoLuc® Luciferase from a rather dull 19kDa... by NanoLuc® luciferase (NLuc) and set about to create an in vivo imaging reporter by linking two CyOFP1...-Lanterns with NLuc and called their improved creations Enhanced Nano-Lanterns. These great imaging tools...the two fluorescent proteins together thereby increasing their FRET signal. FRET is a non-radiative transfer...overlap with this optogenetic tool, they needed to create a calcium sensor with the following characteristics...

...Natsume et al., 2016). Now, the Kanemaki lab has created a new toolkit of plasmids for the N- or C- terminal...makes it easy to tag your protein of interest to create conditional human cell lines. Lastly, the Kanemaki...sites adjacent to an NG PAM in human cells. The increased versatility of the engineered SpCas9-NG and its...enhancers, or have attempted to engineer loops to create genomic interactions that wouldn’t normally occur...develop dCas9 for synthetic looping, the Phillips-Cremins lab developed the light-activated-dynamic-looping...were able to observe loop formation as well as increased Zfp462 expression within 4 hours of blue light...

...self-assembling split GFP (GFP1-10 and GFP11) to increase fluorescence 10-fold after protease activity (... (To et al., 2016). However, the lab wanted to increase the fluorescence range to better study the spatio-temporal...β-strand of GFP, hence the name FlipGFP. FlipGFP increases fluorescence 100-fold compared to other reporters...can be applied to other fluorescent proteins to create an array of multicolor reporters that will enable...apoptosis during embryo development. GFP fluorescence increased over time and showed a clear pattern in the developing... This reporter achieves approximately 100-fold increase in fluorescence after cleavage by executioner ...

...CRISPR pooled gRNA libraries that can be used to screen for genes involved in a wide variety of cellular...processes. While these are widely requested and incredibly useful tools, they require extensive background... infect target cells, and, finally, conduct the screen. To help alleviate some of this labor for our ...optimization-scale infection and at least one screening-scale experiment achieving the gRNA representation...) says: "When you're setting up to do a genetic screen, you need to focus on building a really good model...use along with lentiviral packaging plasmids to create your own lentivirus stocks. Our research scientists...

...could mean needing to increase crosslinking to counteract the effects of increased sample agitation; a ...such as qPCR or next-gen sequencing. ChIP is an incredibly useful application for exploring these interactions...crosslinking, which uses formaldehyde or UV light to create strong but reversible bonds. This will keep the... necessary for most ChIP experiences, but the increased binding can mask the epitopes antibodies recognize...shorter sonication time could be an opportunity to decrease crosslinking. (Homogenization for a tissue sample...