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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 928 results for: Gene

Showing: 231 - 240 of 928 results

  1. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ... certain guide sequences - using 5 guides/gene for 10 genes, they did not observe differences in cleavage... BELOW Biologists are going gaga over the newest gene-editing protein - a DNA-cleaving Argonaute from ... original sequence. If you’re looking to disrupt gene function via indel formation, this property could...Natronobacterium gregoryi, or NgAgo for short. Addgene has already distributed this plasmid all over the...supplied. Find Plasmids from the NgAgo Publication at Addgene Cas9 and NgAgo head to head NgAgo has a number ...27136078. Find plasmids from this publication at Addgene. 2. Swarts, Daan C., et al.  “DNA-guided DNA interference...PMC4697943. Find plasmids from this publication at Addgene. 3. Swarts, Daan C., et al. “Argonaute of the ...

  2. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    Published
    June 15, 2016, 1:30 p.m.
    ...developed as well as CRISPR-based gene repression tools to find and test genes that may play a role in the ...proteomic tools to discover genes and proteins associated with pathogenesis. In his future work. Dr. Bengyella...in China Additional Resources on Addgene.org Learn About Addgene's Mission Check Out Our International...As we mentioned a few months ago, Addgene has been working with Seeding Labs to provide plasmid grants...and Duedu and how they plan to use plasmids from Addgene to advance their research. Louis Bengyella Dr. ...that enable E. coli and Citrobacter freundii to generate sugars from cellulosic biomass. Dr. Duedu would...would like to use the plasmids he acquires through Addgene to produce molecular biological tools like Taq ...

  3. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ... Strong expression: A CMV promoter drives robust gene expression in mammalian cells. Flexible tagging:...tag. Selectable and stable: A puromycin resistance gene makes stable cell line creation a breeze. Modular...labeling By Alyssa Shepard To fully understand how genes are regulated, it's important to know what proteins...identify both new and known regulators at the FOS gene promoter and the MYC locus (Figure 5). Figure...identification of heat-shock proteins at the FOS gene promoter using TurboCas, streptavidin immunoprecipitation...very quick and efficient way to learn more about gene regulation. Find the TurboCas plasmid here! Cenik...Enhancer-AAVs targeting interneurons By Mike Lacy Targeting gene expression to specific cell types in the brain can...

  4. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...Precious Tools to Improve Gene Modification of Hematopoietic Cells for Research and Gene Therapy. Viruses 12...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ... plasmid which contains a promoter, the envelope gene, and a polyA tail.   Figure 1: Lentiviral...can’t be used for neuronal tracing or peripheral gene delivery. Combining the rabies glycoprotein into...Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods 28:291–301 . https://doi.org/10.1089...Resources on Addgene.org: Check out the lentivirus guide Find ready-to-use lentiviral preps at Addgene ...your viral vector, you use the same protocol for generating lentivirus, but you use a different envelope ...

  5. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...cell lines that stably express a gene of interest. While the general approach for using lentivirus, infect...multiplicity of infection (MOI) for predicting gene transfer events.” Genetic Vaccines and Therapy 2.1 (2004) Pubmed...Mycoplasma infection significantly alters microarray gene expression profiles.” Biotechniques 35.4 (2003)....Downstream processing of oncoretroviral and lentiviral gene therapy vectors.” Biotechnology Advances 24.3 (2006...production of lentiviral and retroviral vectors.” Human Gene Therapy Clinical Development 25.3 (2014). 175-85...lentiviral vectors through pseudotyping.” Current Gene Therapy 5.4 (2005). 387-98. Pubmed PMID: 16101513... cells do not take up nucleic acid or express transgenes as well as actively dividing cells resulting ...

  6. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually own the majority ...distribution for their plasmids. MTAs at Addgene Since Addgene was founded in 2004, we’ve handled the majority...If you’ve deposited or requested plasmids from Addgene, then you’ve been involved in the material transfer.... The UBMTA, which is the MTA most plasmids at Addgene are shared under, is well-understood and accepted...for managing research use agreements like MTAs.  Addgene does a lot to ensure that we can facilitate a fast...signing of MTAs by the Provider and Recipient (in Addgene parlance, the depositor and requestor) organizations...that differs.  When you deposit a plasmid with Addgene, your organization’s tech transfer office is sent...

  7. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ... at Addgene's booth, #3422, in the nonprofits section.  The Booth What will you find at Addgene's booth...scientific curation manager, Angela, experts on the Addgene repository, are also attending! Finally, our team....  You'll also find tons of information about Addgene's ready-to-use antibodies, viral vectors, and, of...course, plasmids! Plus, we'll be giving away fun Addgene swag for the duration of the conference (be warned...

  8. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    .... More than half of these sequences were generated by Addgene as part of our QC process. Automation and...From the beginning, one of Addgene’s goals was to ensure the scientific community obtained high-quality...the years to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions...sequencing at our facility using Illumina MiSeq Next Generation Sequencing (NGS) technology. As our repository...will describe the importance of bioinformatics at Addgene and how our search for solutions led us to a partnership...bioinformatics needs       Fig. 1: QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow...our software, even with continuous updates. The generous donation of a Illumina MiSeq sequencer enabled...

  9. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...intended. With the use of applications like SnapGene and Geneious, I was able to determine if any discrepancies...able to explore other parts of Addgene. This was done through the “Addgene 101” series, where each team ... Communication function at Addgene. Working a 9-5 As it turned out, Addgene uses something called “core...completing their co-op at Addgene. This is her second contribution to the Addgene blog.      More resources...brought me, a second year bioengineering student, to Addgene. I am currently working on the QC/QA team as a ...specific overview. It was immediately clear that Addgene wanted to ensure applicants understood what the...that the two hours were split up between several Addgene employees, who all made the daunting task feel ...

  10. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...to knock-out single genes in clonal populations and to knock-out thousands of genes in mixed population...targeting over 19,000 and 20,000 human and mouse genes respectively, use lentiCRISPR v2 as the backbone...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read...announce that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...Availability and Use LentiCRISPR v2 is available from Addgene both as a single empty vector and in pooled libraries...reagents available to the research community through Addgene and look forward to another great year of sharing... Plasmids to Mammalian Cells Resources on the Addgene Website Visit our Lentiviral Guide Pages Find ...
Showing: 231 - 240 of 928 results