We narrowed to 432 results for: sequence

...pH can denature, break, and even change the DNA sequence. High pH favors the breakage of the hydrogen bonds...backbone of the DNA helix or even changes the DNA sequence through a mechanism called depurination. In depurination...

...addition to peer review than having your plasmids sequenced and openly registered for other scientists to ...we won’t distribute materials with unresolved sequencing issues or if you’ve asked to cancel the deposit...

...scores provided by authors rather than sgRNA-level sequence read scores. The original supplemental data tables...query screen data without the need to analyze raw sequence data.  This format is inherently flexible and ...

...series. Single DSBs resolved by NHEJ at coding sequences often result in loss-of-function frameshift mutations...analysis, allele-specific PCR, T7EN1 cleavage assay, sequencing, RT-qPCR, or immunoblotting to detect. Another...

...targets 18,080 human genes with 64,751 unique guide sequences to enable both negative and positive selection...proof-of-principle that CRISPR-Cas9 can be used to target any sequence in the genome. Tips and tricks Wang offers this...

...the cap plasmid library by inserting short DNA sequences that contain all possible combinations into a ...plasmid, loxP sites flank the downstream polyA sequence. When viruses produced from this plasmid pool ... animal with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing tissues...

...Pennsylvania independently searched for this unique sequence of DNA and both identified lymphocyte antigen ...as a receptor for PHP.B by looking for a unique sequence of DNA that was present in mice whose CNS was ...alleles that was driving the PHP.B phenotype. Exome sequencing of related hybrid mice narrowed the search to...

...ten base pairs in length. An emerging family of sequencing techniques function as a kind of “GPS for the...over time, we periodically use next-generation sequencing to measure the relative proportion of guide RNAs...insulators, and more. I then identified valid gRNA sequences without off-target effects for the 2+ million ...

...antibodies will come with access to the antibody sequences, in line with the rest of Addgene’s recombinant...

...Rosella. When fused with a mitochondria-targeting sequence, Rosella can be used to track the targeted autophagy..., but the addition of a mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It...