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We narrowed to 885 results for: ANT

Showing: 691 - 720 of 885 results

  1. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...handle, but I wasn’t using them to my professional advantage. It wasn’t until I wrote a blog post about a research...start using social media to their professional advantage, I’ve selected five resources that have tips for...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you...

  3. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ... a variety of common plasmid features such as antibiotic resistance genes, selectable markers, promoters... files Once you’ve identified a plasmid that you want to learn more about, updates to our sequence analyzer...used to indicate them on the map, direction (if relevant), and types. Types listed are standard GenBank...

  4. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...  Multiplexing Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate ...Multiplexing crRNA expression with Cpf1  The key advantage of multiplexing crRNA expression with Cpf1 is ...

  5. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...self-supporting service so that Addgene is not dependent on grants. Aiming to operate under a similar model for our...word out about this new resource and providing fantastically useful educational content on viral vectors.... Addgene’s Legal Team acquired permission from relevant plasmid depositors and their institutions to distribute...

  6. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...polymerase. E4/E1A activate transcription of other relevant viral genes (including the ones listed previously... it to evade detection by the host. Of note, recombinant adenovirus is missing the endogenous E1 gene,...services for some of the plasmids in our collection. Want to make a vector construct on your own? No problem...

  7. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...allows you to infect cells and deliver cargo, it’s important to understand their differences to choose the ...used to achieve transient, high expression of the relevant cargo without the fear of random genomic integration...non-integrating and infect non-dividing cells. AAVs primary advantage over adenovirus is their low immunogenicity, which...

  8. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...structures at the ends of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase...homologous recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge SJ. Nat Methods....

  9. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ... virus is produced in just a few days. Another variant, Phoenix-gp, contains only Gag-Pol and enables ...γ-retroviruses differ from lentiviruses? There are a few important differences between engineered γ-retroviruses ...names? The nomenclature of the plasmid backbone is meant to be descriptive of the components found within...

  10. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...The lab additionally recommends using an anti-mCherry antibody rather than endogenous mCherry fluorescence...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...

  11. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...may seem tedious at first, especially since you’ll want to dig in to the computation, but they will serve...specific goal for each analysis will help you locate relevant information in the future. Approach: Outline...can store any power point, excel, or other files relevant to the project. README: The README.txt document...

  12. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    Published
    Aug. 16, 2018, 12:40 p.m.
    ...  and that’s just to name a few. But what if you want to study RNA-protein interactions? The characterization...RNAs and proteins with UV light, followed by an antibody pulldown of a protein of interest, much like using...disease. RaPID can also be used to test how RNA variants associated with human disease affect protein binding...

  13. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ...riboprobe binding (via hybridization) specific antibodies that detect the mRNA-riboprobe complex are added...stain the cells that contain this mRNA-riboprobe-antibody complex and, as a result, these cells turn blue...Addgene. If you use these published protocols and want to share pictures, feedback etc. please write to...

  14. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ... deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to ...Mature Nanoblades then bud into the cell culture supernatant and can be purified by centrifugation. Gag-Cas9...

  15. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...publication.   3’UTRome The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans...Dynamics Database (WDDD) The WDDD aims to acquire quantitative data on all the cell divisions of the C. elegans...lab in 2015 adapted the AID system discovered in plants to conditionally deplete proteins of interest in...

  16. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...active in vitro and in human and plant cells. Its small size offers advantages for cellular delivery. Get ...integration and connectivity without the constraints of antibody labeling. This opens the door to in vivo experiments...

  18. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...scientific conference or a karaoke bar), you probably want to sigh. The problem is that many of these studies...Sapozhnikov and Szyf 2021, Charlton et al. 2020). Most importantly, TET proteins affect transcription independent... directly inside the gRNA sequence is a great guarantee that DNMT1 will be blocked (within PAM works, ...

  19. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ... Zhang’s lab characterized two Cpf1 nucleases, distant cousins of well-known Cas9. Cpf1 cleaves DNA in...2015). PubMed PMID: 26422227.   Potential advantages of Cpf1 Type II CRISPR systems based on Cas9 ...efficiency across CRISPR nucleases and NgAgo is an important next step for the field. Based on what we know...

  20. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...poster you want to know more about? Interested in doing a postdoc in a presenter’s lab? Want to collaborate...breaths the day-of, you’ll handle them with ease. Anticipate questions: When practicing with your colleagues...

  21. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...with all these CRISPR plasmids (and libraries)? We wanted to know, so we classified CRISPR plasmids containing...other countries, from Japan to Australia, are significant depositors and requestors. CRISPR tools are developed...our CRISPR depositors and requestors. Thank you!  Want to dive deeper into Addgene’s CRISPR data? Read ...

  22. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ...difficult” GC-rich regions of the genome, which seem resistant to Cas9 cleavage (1). Both the CRISPR-Cas9 and...researchers were unhappy with the new method and 65% wanted to wait until someone else optimizes an NgAgo protocol...wouldn’t be a surprise if someone came up with a mutant NgAgo (or a novel endonuclease) protein that can...

  23. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license. Figure 2 gives a...is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential...1406488. PubMed Central PMCID: PMC372876 Dong, W., & Kantor, B. (2021). Lentiviral Vectors for Delivery of ...

  24. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    Published
    Aug. 9, 2016, 2:30 p.m.
    ...(ESCs). Your ChIP-seq finds just over 13,000 significant binding peaks for NANOG in the human genome. .... But not all of those 13,000 regions are important for maintaining ESCs. So which of these 13,000 regions...cells compact DNA in a way that brings linearly distant regions close together. gRNA Selection and Filtering...

  25. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...After sequencing and searching for cellular RNAs important for Cpf1 function, they found that mature crRNAs...staggered pattern similar to FnCpf1. Potential advantages of Cpf1 over Cas9 Type II CRISPR systems based... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA...

  26. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... the Addgene repository can save researchers significant time and removes a variable from the lengthy ...from 2016–2023 by type of tool. Equally as important to what is being delivered is where it’s being...trends in our own repository data, giving us significant insight into which vectors are likely to be of...

  27. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...fluorescent protein control. We also added the toxin-antitoxin stability cassette Axe-Txe so that the plasmid...plasmid could be maintained in the absence of antibiotics, resulting in the plasmid pBAD-bARGSer-AxeTxe (Fig...agents to boost expression to appreciable levels. We wanted to create second-generation mARGs with higher expression...

  28. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...continue to rely on printed journal articles as the dominant tool for sharing ideas. This leads to everyday...platforms (such as social networking, visual appeal, instant sharing across multiple other sites), I created...learn about the results of studies on reducing antibiotic prescriptions for respiratory tract infections...

  29. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...biochemical assays followed by in vivo testing. The mantra being “you can’t trust those whacky cell lines”...contamination with HeLa being the most frequent contaminant, closely followed by T24 bladder cancer cells...repository like the ATCC, there are times when it is important to check their identity. For example, perhaps ...

  30. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...fragments are likely non-functional, they could be dominant-negative and/or toxic. A potential solution is..., was 81% of that from a single vector, but significantly greater than the expression from an overlapping...While the hybrid dual vector system has a slight advantage over the overlapping and trans-splicing approaches...
Showing: 691 - 720 of 885 results