We narrowed to 897 results for: ANT

...SARS-CoV-2 were developed for broad use and are now important for accelerating research into this virus. One...scientific community. Addgene was awarded a Fast Grant to ensure that the scientific community will continue...Deposits of new reagents, policies from journals and granting agencies requiring materials to be deposited into...

...be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment.  ...Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but much of...valid email address).  One may also simply attach important references that serve to further characterize ...

...CRISPR tools, without compromising their strategic advantages in gene editing, while keeping the system to ... array with a given tRNA (derived from human or plant source), by tuning the length of 5’ leader sequences...outperformed other tRNA candidates (either from human or plant sources) in both the strength as a promoter and ...

...screen and transform your work into a paper or a grant. Here’s a list of tools and resources that might...differences between these two citation managers. If you want more tips on finding and organizing papers, check...post: Early Career Researcher Toolbox: Finding Relevant Papers. These are only a few of the many writing...

... many aspects of ApoB-LP biology, including quantification of abundance, size, and localization in vivo...dependent and the system may not be adaptable to distantly related species from E. coli. This tool should...be performed in intact animals and offers many advantages over traditional measurements such as patch clamp...

...be of secondary importance (or tertiary, or non-existant). On the very first night of the iPSC symposium... the stem cell community for addressing these important questions that the scientific method cannot address... it took the joint effort of five post-docs to implant human kidney progenitors into a pig. In stark contrast...

...get more done. There are also a host of other advantages to practicing effective delegation skills: ...responsibility if things go wrong. Here are some important criteria for delegating tasks appropriately: ... and accountability? What types of issues do you want to know about right away? Provide adequate support...

...understanding of the topic will give you some advantage over other candidates. Write about your transferable...research. For example, did you help put together a grant application? Are you familiar with the publishing...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...

...cloning: A SLIC makeover In 2007, LIC received an important update, courtesy of Addgene depositor Stephen ...structures at the ends of fragments. The major advantage of SLIC over Gibson assembly is cost, as T4 polymerase...homologous recombination in vitro to generate recombinant DNA via SLIC. Li MZ, Elledge SJ. Nat Methods....

... virus is produced in just a few days. Another variant, Phoenix-gp, contains only Gag-Pol and enables ...γ-retroviruses differ from lentiviruses? There are a few important differences between engineered γ-retroviruses ...names? The nomenclature of the plasmid backbone is meant to be descriptive of the components found within...

...polymerase. E4/E1A activate transcription of other relevant viral genes (including the ones listed previously... it to evade detection by the host. Of note, recombinant adenovirus is missing the endogenous E1 gene,...services for some of the plasmids in our collection. Want to make a vector construct on your own? No problem...

...allows you to infect cells and deliver cargo, it’s important to understand their differences to choose the ...used to achieve transient, high expression of the relevant cargo without the fear of random genomic integration...non-integrating and infect non-dividing cells. AAVs primary advantage over adenovirus is their low immunogenicity, which...

... a variety of common plasmid features such as antibiotic resistance genes, selectable markers, promoters... files Once you’ve identified a plasmid that you want to learn more about, updates to our sequence analyzer...used to indicate them on the map, direction (if relevant), and types. Types listed are standard GenBank...

...  Multiplexing Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate ...Multiplexing crRNA expression with Cpf1  The key advantage of multiplexing crRNA expression with Cpf1 is ...

...self-supporting service so that Addgene is not dependent on grants. Aiming to operate under a similar model for our...word out about this new resource and providing fantastically useful educational content on viral vectors.... Addgene’s Legal Team acquired permission from relevant plasmid depositors and their institutions to distribute...

...The lab additionally recommends using an anti-mCherry antibody rather than endogenous mCherry fluorescence...transduction with this virus. While various Cas9 mutants have been engineered to perform unique editing ...

...updated Jul 20, 2021. By Will Arnold and Shreya Vedantam Accelerating research by improving the availability...expression vectors containing various spike protein variants are also available. Plasmids are continually added...these plasmids available for scientists speedily meant that we had to revamp some of our quality control...

...immunogenicity. But these studies also identified other advantages, including Cas13d-specific regulatory proteins... makes WYL proteins an intriguing counterpart to anti-CRISPR proteins that negatively modulate the activity...efficiency; where shRNA treatment produced 500-900 significant off-targets, CasRx displayed zero. Unlike Cas9...

... 1990’s, microRNAs (miRNAs) are recognized as important regulators of post-transcriptional gene expression...in vivo, the non-immunogenic violet-excited GFP variant Vex. To understand the impact of this gene editing...immune response other than what would be the anticipated effect of the targeted gene. CHIME can also be...

...handle, but I wasn’t using them to my professional advantage. It wasn’t until I wrote a blog post about a research...start using social media to their professional advantage, I’ve selected five resources that have tips for...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you...

...may seem tedious at first, especially since you’ll want to dig in to the computation, but they will serve...specific goal for each analysis will help you locate relevant information in the future. Approach: Outline...can store any power point, excel, or other files relevant to the project. README: The README.txt document...

...innovations for therapeutic and vaccine design. We all want tools that expand what we can achieve, but are simple...lot of left-over starting components. High rate constant— time is money and things move fast in biology...fluorescent proteins. So, I hope you will take full advantage and have lots of fun building and clicking. References...

...  and that’s just to name a few. But what if you want to study RNA-protein interactions? The characterization...RNAs and proteins with UV light, followed by an antibody pulldown of a protein of interest, much like using...disease. RaPID can also be used to test how RNA variants associated with human disease affect protein binding...

...active in vitro and in human and plant cells. Its small size offers advantages for cellular delivery. Get ...integration and connectivity without the constraints of antibody labeling. This opens the door to in vivo experiments...

...where you want it to? Knowing that a gene is expressed in a particular cell type is important not only ...Gross-Scherf, B., Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke...

...riboprobe binding (via hybridization) specific antibodies that detect the mRNA-riboprobe complex are added...stain the cells that contain this mRNA-riboprobe-antibody complex and, as a result, these cells turn blue...Addgene. If you use these published protocols and want to share pictures, feedback etc. please write to...

...scientific conference or a karaoke bar), you probably want to sigh. The problem is that many of these studies...Sapozhnikov and Szyf 2021, Charlton et al. 2020). Most importantly, TET proteins affect transcription independent... directly inside the gRNA sequence is a great guarantee that DNMT1 will be blocked (within PAM works, ...

...publication.   3’UTRome The 3’UTRs of mRNA are important for post-transcriptional regulation in C. elegans...Dynamics Database (WDDD) The WDDD aims to acquire quantitative data on all the cell divisions of the C. elegans...lab in 2015 adapted the AID system discovered in plants to conditionally deplete proteins of interest in...

... deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are fused to ...Mature Nanoblades then bud into the cell culture supernatant and can be purified by centrifugation. Gag-Cas9...

... Zhang’s lab characterized two Cpf1 nucleases, distant cousins of well-known Cas9. Cpf1 cleaves DNA in...2015). PubMed PMID: 26422227.   Potential advantages of Cpf1 Type II CRISPR systems based on Cas9 ...efficiency across CRISPR nucleases and NgAgo is an important next step for the field. Based on what we know...