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  1. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ...written content about timely scientific topics, as well as archived seminar presentations. One of SITN'... In return, the public receives trustworthy, intelligible scientific resources. SITN hopes to make DayCon...

  2. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    Published
    June 17, 2014, 7:57 p.m.
    ...spatio-temporal control of gene expression within a cell. Thanks to the work of Huimin Zhao and colleagues...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...

  3. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ... brain regions. You can partially control which cells are successfully infected by an AAV by altering ...control where your gene of interest is expressed.  A cell-type specific Cre can provide you with that control...

  4. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ... an Addgenie Series on Instagram. I interviewed fellow Addgenies about their role in the company. One ...Addgene. This is exemplified in my work interviewing fellow Addgenies. I spoke with amazing people that were...

  5. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...mixture of two base editor enzymes in mammalian cells. Read the paper in bioRxiv Find Target-ACE   CRISPR...bionanoparticle Transient expression of CRISPR/Cas9 in cells has advantages like reduced off-target activity ...

  6. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...pathways that are frequently implicated in cancer cell proliferation, survival, differentiation, and apoptosis...pathway. “The idea is that if you introduce them into cells one at a time, you could identify those that confer...

  7. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...insertion. The linear PCR product is introduced into the cell by direct transformation. A given insert will typically...protein-protein interactions via FRET. Sidae Lee, Wendell Lim, and Kurt Thorn at UCSF have recently developed...

  8. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

    Type
    Blog Post
    Published
    June 8, 2023, 1:15 p.m.
    ...upload and share their negative data. After all, they tell me, when something doesn’t work, it’s not a failure...approach for deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National ...

  9. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...small Cas protein, is active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered ...demonstrating that CasX is active in both E. coli and human cells. Using the structure of CasX at eight different ...

  11. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ..., and VP3, which together form the outer capsid shell that protects the viral genome. The cap gene is ...for serotype-specific differences in tissue and/or cell type tropism. Introducing changes and/or short peptides...

  14. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    Published
    April 25, 2023, 1:15 p.m.
    ... region of a different species and/or isotype (Liddell 2013). In most cases, the resulting chimeric antibody...antibodies! References and resources References Liddell, E. (2013). Antibodies. In The Immunoassay Handbook...

  16. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    Published
    Oct. 20, 2020, 1:15 p.m.
    ... or more “important”, they just need to know you well enough to ask good questions and hold you accountable... can share it with others and get their input as well. Giving a great video presentation is going to be...

  19. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ...industry?” Once academia was the clear answer with a well-defined path to professorship. But recently a downward... professions increases your chances of finding a well-suited job. EAs shouldn't be viewed as an unproductive...

  20. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...allow scientists to express a gene of interest in a cell or animal model. AAV plasmids contain several important...Integrity on rAAV8 Production Using the Baculovirus/Sf9 Cells System. Hum Gene Ther Methods, 28(5), 277–289. https...

  21. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...skip purification steps and end-repair steps with well-designed fragment overlap sections, while still ...of NEBuilder HiFi DNA assembly method As with any well-executed cloning endeavor, NEBuilder requires upfront...

  22. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...measure the pH of our samples, we used a solution of cell culture medium, which is buffered and contains the... 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer P, Manning MC, Fitchmun MI. Protein...

  23. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...Addgene deposits? Even more interestingly, could we tell if depositing a plasmid with Addgene causes a paper... most relevant to plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry...

  24. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...hard-to-read sentences.  Grammarly Grammarly is a lot like spell check, but since it’s an internet browser plug-in...managers and productivity tools for grad school Hello PhD Podcast episode 15 "Simple tricks for time management...

  25. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    Published
    Jan. 12, 2021, 2:15 p.m.
    ...again or set up a meeting. If you really got along well, suggest a coffee chat in the near future. Even ...you succeed in any way. Don’t believe people who tell you that thank you isn’t necessary. I once got a...

  26. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...Director of Quality Control Jonathan Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction...fantasy, pop-sci, true crime, and so on. We’ve read well-known classics, like Austen’s Pride and Prejudice...

  27. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...for the next 5 years. Does an academic career work well with your life and career plan? This might be a ... - includes videos on general resume writing, as well as additional videos with emphasis for graduate ...

  28. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...professors how they hire scientists for their labs. Tom Ellis selects new hires from a pool of CVs collected from... awhile. Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

  29. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...requirements in regard to their sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or...References References Adler, B., Trinidad, M., Bellieny-Rabelo, D., et al. (2023). CasPEDIA Database: ...

  30. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...quantitatively evaluating antibody staining, or counting cells are now routine, open-source software projects that...portable device for studying plankton using flow cells, has become an established alternative to high-priced...
Showing: 691 - 720 of 841 results