We narrowed to 786 results for: KIN

1. Viral Vectors 101: Parts of the AAV Packaging Plasmid

   Type

   Blog Post

   Published

   Dec. 3, 2020, 2:15 p.m.

   ...of course! You just need three plasmids to start making the AAVs you need for your experiment:  the packaging...

2. Sleeping Beauty Awakens for Genome Engineering

   Type

   Blog Post

   Published

   June 30, 2015, 2 p.m.

   ... of a mirrored set of inverted repeats (green) flanking the gene of interest (blue) in a plasmid backbone...

3. History of CRISPR Cas - A tale of survival and evolution

   Type

   Blog Post

   Published

   Nov. 25, 2013, 10:12 p.m.

   ...Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata A. J Bacteriol. 1987 Dec; ...

4. New Viral Vectors - Winter 2025

   Type

   Blog Post

   Published

   Jan. 28, 2025, noon

   ...Fishell New viral prep AAVrg = AAV retrograde Looking for more viral vectors? Browse our in-stock viral...

5. Multifunctional Neural Fibers: Modulation from Gut to Brain

   Type

   Blog Post

   Published

   Aug. 31, 2023, 1 p.m.

   ...influenced by signals it receives from the entire body, making it difficult to probe due to the lack of tools ...

6. 10 Great Guest Posts We're Thankful For

   Type

   Blog Post

   Published

   Nov. 18, 2016, 3 p.m.

   ...understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS” In this post, Albert Cheng and Mark...

7. Using Addgene's New Viral Service for Your Research

   Type

   Blog Post

   Published

   Nov. 17, 2016, 3:30 p.m.

   ...website. blog.addgene.org The blog provides tips for working with viruses including advice on how to titer lentiviral...

8. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

   Type

   Blog Post

   Published

   June 22, 2021, 1:15 p.m.

   ..., Tegally H, Lustig G, Giandhari J, Pillay S, Wilkinson E, Naidoo Y, Karim F, Ganga Y, Khan K, Bernstein...

9. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

   Type

   Blog Post

   Published

   Nov. 8, 2018, 3:47 p.m.

   ...whether a gene is natural or synthetic just by looking at its sequence. MIT collaboration for gene classification...

10. CRISPR Challenges: Standardization and Homology Directed Repair

    Type

    Blog Post

    Published

    Feb. 14, 2018, 2:36 p.m.

    ...genome editing efficiency. There is so much more to working with CRISPR than just designing a repair template...

11. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type

    Blog Post

    Published

    April 23, 2020, 1:15 p.m.

    ...about Addgene's viral service Find protocols for working with virus ...

12. New and Upcoming Viral Vectors - December 2019

    Type

    Blog Post

    Published

    Dec. 19, 2019, 2:40 p.m.

    ...pAAV-hSyn-DIO-EGFP   Biosensor AAV Calcium sensors Tracking calcium dynamics that govern such activities as...

13. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type

    Blog Post

    Published

    Nov. 12, 2019, 2 p.m.

    ... Mork collecting samples at the hot spring. Hiking all day and processing samples by night was fun...

14. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type

    Blog Post

    Published

    Sept. 18, 2018, 1:33 p.m.

    ...Quantification of editing efficiencies simply involves tracking the ratio of mCherry-positive to eGFP positive...

15. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    Published

    Oct. 10, 2019, 12:41 p.m.

    ...large, dim, and permanently attached to a protein, making them hard to amplify and image for long periods...

16. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type

    Blog Post

    Published

    Sept. 5, 2019, 1:06 p.m.

    ...legal agreement. Below, you will find some easy talking points for broaching this subject with your institution...

17. Another Pathway into Cells: iTOP

    Type

    Blog Post

    Published

    June 23, 2015, 8:37 p.m.

    ...safety concerns associated with virus work. Fourth, working directly with proteins removes the limitations ...

18. Addgene Depositors Get More Citations

    Type

    Blog Post

    Published

    April 20, 2017, 2:30 p.m.

    ... argument that Addgene is achieving its goal of making plasmid-based science more accessible…and accruing...

19. Visualizing Genomic Loci with CRISPR-Sirius

    Type

    Blog Post

    Published

    April 18, 2019, 12:53 p.m.

    ...predecessor CRISPRainbow, CRISPR-Sirius is brighter making it suitable for observing low-copy genomic loci...

20. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

    Type

    Blog Post

    Published

    Nov. 20, 2018, 8:17 p.m.

    ...changes) the original identifier loses its meaning, making it difficult or impossible for researchers to track...

21. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type

    Blog Post

    Published

    July 21, 2020, 1:15 p.m.

    ...contamination based on the presence of other bands or streaking. Unlike absorbance based methods, this method ...

22. DAP arrays for multiplex gene editing

    Type

    Blog Post

    Published

    July 7, 2022, 1:15 p.m.

    ...generate multiple gRNAs for nCas9-BE, or any other kinds of CRISPR tools, without compromising their strategic...

23. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type

    Blog Post

    Published

    June 20, 2017, 2:30 p.m.

    ...We’re always looking for ways to improve our shipment processes. After reading a publication describing...

24. 5 Great Apps for Lab Life

    Type

    Blog Post

    Published

    Sept. 22, 2016, 2:30 p.m.

    ...with research! It is always important to know what kind of reagents you work with in experiments. Hence,...

25. Management for Scientists: Delegating is Key

    Type

    Blog Post

    Published

    Feb. 20, 2015, 8:30 p.m.

    ...roles leads to more ideas, initiative and creative thinking. The team members will be more invested in the...

26. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type

    Blog Post

    Published

    Oct. 9, 2014, 3 p.m.

    ...infrared channels used for more abundant proteins or marking compartments. I hope this sheds some light on multicolor...

27. Viral Vectors 101: Types of viruses

    Type

    Blog Post

    Published

    June 6, 2023, 1:15 p.m.

    ...also transduce dividing and non-dividing cells, making it conducive to targeting all cell types. Altogether...

28. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...activate the DREADD, CNO is delivered either through drinking water or intravenously. Tips for using pAAV-hSyn-DIO-hM4D...

29. Hot Plasmids - February 2022

    Type

    Blog Post

    Published

    Feb. 22, 2022, 2:15 p.m.

    ..., including live-cell imaging, single-molecule tracking, immunolabeling of fixed cells, Western blotting...

30. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    Published

    Feb. 2, 2021, 2:15 p.m.

    ...compelling narrative that is positive, and forward-looking. Embrace it, and let your personality leap out ...