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  1. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...Adapted from Zhang et al. 2023 with permission.   Find Cas-PAGE plasmids here!   Zhang, Z., et al....

  2. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...associated with low morbidity and mild symptoms. This changed in January with the report of an outbreak of the...Learn How Minigenomes Are Being Used to Study Dangerous Viruses Resources on Addgene.org Check out All...

  4. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...stranded breaks, in which nucleotide sequences are exchanged between two similar or identical molecules of ...and animal models, their efficiency is very low, ranging from undetectable to 0.1%. In contrast, new genome...

  5. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...background relative to your research? Tailor your language to your audience so that they can digest what ... contact with the presenter after the session. Exchange business cards or jot down their name and affiliation...

  6. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...infects host cells through binding the human angiotensin converting enzyme 2 (ACE2) cell-surface receptor....01.30.428974   The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids...

  7. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... DNA in a number of ways, from small edits that change single base pairs, to chromosomal inversions and... S., Costa, F., Shah, S. A., Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, ...

  8. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    .... V., Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J...–375. https://doi.org/10.1038/s41586-023-06570-y Wang, J., Gessler, D. J., Zhan, W., Gallagher, T. L.,...

  9. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...titration by qPCR.   Pro tip! An optional media exchange can be performed 12–18 hours post-transfection...the density gradient material by dialysis/buffer exchange and further concentrate the virions if needed....

  10. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...of an exogenous small molecule). Degron tags can range in size from several kDas up to ~35 kDa and are ...10.1038/s41467-022-33246-4 Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, Lin MZ. Tunable...

  11. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...same type will produce the same unique SapI 5’ overhang, different combinations of connector and tag donor...interactions. Additionally, thanks to the modular arrangement of vectors included in tagging systems like SapTrap...

  12. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...targeted modifications. A recent publication by Zhang et al. shows just that - using MMEJ to insert FLAG... plasmids from this publication at Addgene. 3. Zhang, Chi, Xiuhua Meng, Xiaolei Wei, and Ling Lu. (2016...

  13. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...factor IGF2 open reading frame to relax imprinting (Zhang et al., 2017). This upregulates expression of IGF2...PMID: 23452860. PubMed Central PMCID: PMC3664290.  Zhang, Yiqun, et al. "CRISPR Cas9-guided chromatin immunoprecipitation...

  14. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...imposed on scientists. Figure 6: Model fit. Orange line depicts observed data. Blue line is the sum...depending on when we believe the China COVID-19 date range is, they could be the most impacted or least impacted...

  15. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...answers hundreds of AAV questions every year on a range of issues related to AAV vectors, many from people...specifically to allow scientists to evaluate how small changes in vector administration can influence AAV expression...

  16. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ... Percent of Neurons Transduced   Dorsal Root Ganglion (DRG) AAV.9 (parental) 46 +/- 0.7% AAV.PHP.S...figure 8 in Chan et al).  References 1. Chan, KY, Jang MJ, Yoo BB, et al. Engineered AAVs for efficient...

  17. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ... and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated methods, while technically... 29668265. PubMed Central PMCID: PMC6002863. 4. Liang, Zhen, et al. "Efficient DNA-free genome editing...

  18. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...college student searching couch cushions for spare change, desperate to extract a few more photons from a...improved brightness, photostability, and spectral range. Nevertheless, these fluorophores were designed ...

  19. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s lab) is an example of the former. In this system...While this may be difficult to resolve for a wide range of prey proteins, there are now a number of two-...

  20. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ... staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from the PAM. With this information... Other CRISPR Blog Posts Additional Resources Zhang lab Cpf1 blog post on Benchling ...
Showing: 461 - 480 of 691 results