We narrowed to 700 results for: EED

...immediately transformed and stored. Some samples need to be transformed with a micropulser using an electric...samples require a reduced antibiotic concentration or need to be transformed into different cells than were...

... with WPRE reduced the number of copies of gvpA needed to increase expression. We were able to express...microscopy and TEM (Fig. 2c), and ultrasound imaging. If needed, resort the cells again for improved purity and...

...processes in C. elegans: mScarlet3 for applications needing intense brightness (such as low-expression proteins...co-imaging. And because bioluminescent markers do not need to be excited by an external light source, they ...

...viral vector selection process to standardize our feedback and evaluation of viral vectors. This process ...as they've helped us.  Interested in providing feedback on AAVs at Addgene? Suggest a viral vector for...

... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...bistable network, stripe pattern-forming incoherent feed-forward loop. Find the plasmids at Addgene! ...

... noticed Holly Bik live-tweeting about topics I needed to know more about. I caught up with her outside...Some of the variability was organic, as some people need less assistance than others, but we did have some...

...companies produce different products and therefore need people with different areas of expertise in technical...unobtainable solutions. To some extent there is the need (or opportunity) to get involved in product testing...

...in new ways. Along with a new location comes the need for new conference room names. The Addgenie’s favorite...understand where sonic hedgehog got its name, we need to go back to 1980, when Christine Nusslein-Volhard...

... gene Y, which has a defective allele Ym and you need to convert an adenine (A) to a guanine (G)? You ...with the endogenous tRNA-processing system." Proceedings of the National Academy of Sciences 112.11 (2015...

...host cell division to plasmid proliferation." Proceedings of the National Academy of Sciences 80.15 (1983... model for programmed bacterial cell death." Proceedings of the National Academy of Sciences 93.12 (1996...

... neuronal synapses for instance. Also these tags need to be fused to scaffold proteins in order to be ...composed of FPs fused to multiple epitope tags. Indeed, to create the smFPs Looger’s team strategically...

... adjacent motif) near the target DNA, TnpBs also need a short sequence motif called a TAM (transposon-... used to generate gene-edited plants without the need for tissue culture (Maher et al., 2020; Qiao et ...

...2014). Scientists from the University of Geneva (Creed et al., 2015) used optogenetics to support these...Psychoneuroendocrinology 46 (2014): 78-87. PubMed PMID: 24882160. Creed, Meaghan, Vincent Jean Pascoli, and Christian Lüscher...

.... But in order to plan a flow experiment, you’ll need to first read papers with flow data... and to read...endless loop. Let’s break out of the loop with all you need to know on how to read a flow plot.  Dot plots ...

...Ligand regulated recombinase - keeping Cre out until needed But what can be done when the recombination should...induced by Cre recombinase expression while proteins needed for later experiments (cellular tracking for instance...

...Submission Guidelines here, but essentially we just needed to send a Google calendar invite over to the r/...a paid account which gives me all the features I need to save, organize and easily find the resources ...

...you may even wish to generate a point mutation and need to focus your attention to that particular region...easier and doesn’t require the extra precautions needed when dealing with RNA (e.g. face mask, and a dust...

...or C-terminal fusion based on your experimental needs. Equally important, before cloning you must consider...dark fusions is likely misfolded, blocked from proceeding through the secretory pathway, and retained in...

...neuronal activation and is a sufficient readout to proceed with your behavioral experiments. Comparison of... to use c-fos expression to get the evidence you need. For example, as shown in this study [3], hippocampal...

...commercial strains designed to avoid them. Why do I need an expression strain? Protein expression from high-copy...number plasmids and powerful promoters will greatly exceed that of any native host protein, using up valuable...