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We narrowed to 856 results for: ELL

Showing: 461 - 480 of 856 results

  1. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...expression targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches...approaches excel at discrete cell or tissue-subregion targeting down to the limits of light delivery. ...-wide targeting, as the ligand can reach target cells throughout the body. Controlling Stimulation Some... and chemogenetic tools need to be delivered to cells, commonly through viral delivery by AAV. You can...Further restricted to illuminated region Impacts all cells expressing receptors Invasiveness Mice require...

  2. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...mammalian cells. Harrington et al tested GeoCas9’s editing skills by transfecting HEK293 cells with GeoCas9...choice for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs...pyogene’s SpCas9. These Cas9 proteins don’t work well at high temperatures, so to use them in thermophiles... 45 °C 50 - 70 °C Genome Editing in Mammalian Cells? Yes Yes Longevity in Serum?* No Yes Table ...experimental results. GeoCas9 RNP editing in mammalian cells Besides being able to take some heat, GeoCas9 can...

  3. Choosing Your Perfect Empty Backbone

    Type
    Blog Post
    Published
    Aug. 19, 2014, 3:39 p.m.
    ... add bells and whistles to your construct. If you are planning to track your protein in the cell or purify..., or express the insert they carry in the target cell. These vectors allow you to test the function of...selection cassette to screen for positive transfected cells. The addition of an in-frame fluorescent fusion ...get away with generating transiently transfected cells, you will not need a mammalian selection cassette.... Other vectors can be expressed in fly culture cells like S2 and carry the metallothionein promoter (...

  4. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...Tom Ellis highlights the importance of keeping up-to-date with the literature of a couple of well-known...yet attainable milestones that contribute to intellectual thought and discovery to the scientific community...ideas. What mentors are available for guidance, intelligent discussion, and/or brainstorming sessions to ...increase chances that your project will be fresh as well.” Don't underestimate the importance of choosing...for you.   Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

  5. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...protein purification as well. This tag is not suitable for use in apoptotic cells as Caspase3/7 both cleave...originally derived from jellyfish (Aequorea victoria) and can serve as an epitope tag as well as a fluorescent...Addgene’s catalog including a mouse monoclonal (M2) as well as a chimera (rabbit). Myc The Myc tag is a 10 ...fluorescent marker. The GFP tag is ideal for live cell imaging, FRET, flow cytometry, or immunofluorescence where...

  6. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ... Below you’ll find brief summaries of several excellent guest posts from our depositors. Interested in...Your Favorite Fluorescent Protein Oligomerizes in Cells GFP oligomerization can lead to misleading artefacts...which specific chemical inputs are received by a cell. These so-called recombinase-based state machines...required for viral replication and transcription as well as reporter genes. To learn how you can use minigenomes...used to deliver a genes of interest to a variety of cell types in-vivo. These are widely used by the optogenetics...activation and repression of different genes in a single cell, and localization of a fluorescent signal to your...

  7. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...community. Addgene's AAV preparations have worked well in my lab, including in applications where we have... can be used to deliver fluorescent proteins to cells as determined by the serotype. You can use these...determine if your AAVs are getting to the right cells using your particular delivery technique and conditions...expanding our viral vector educational resources as well, with both posts on the blog and pages on the Addgene...website you’ll find our updated viral vector pages as well as expanded virus protocols, information on how ...specific material page and includes western blots as well as other expression data. If you have any questions...

  8. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    Published
    Aug. 25, 2015, 2:30 p.m.
    ...would rather fund excellent people with mediocre ideas than mediocre people with excellent ideas. I am lucky...your life making connections and finding mentors -- well, it's time you call upon these people for guidance...Don’t go it alone.  You’ll find that no matter how well you know your idea, you will rarely succeed in launching...partners.  You need a dedicated team that you know well and trust.  This will allow you to discuss each ...two close friends, Sreyan Chowdhury and Clinton Buell, who have very diverse educational backgrounds (...

  9. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...Fhearraigh, PhD, of www.phdadvice.com. I sold out, well in part that’s what leaving academia feels like ...would continue on with my academic dream of studying cell division. However, after 6 years in the business...products, so you have some of the best ideas on how to sell these products to other scientists. Tailoring my...introduction to the financial world. It teaches you about selling products which is an important part of the business...Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial ...information on ELISA troubleshooting, ELISA kits and cell death assays. You can find Seán on twitter @Macfhearraigh...

  10. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    Published
    March 4, 2014, 4:49 p.m.
    ...an awake monkey is different from a cell in a dish. I always tell people, 'Be driven by your science.'...for neuroscience back in 2005, both activators as well as silencers of neural activity. Since then, there...to Addgene in a matter of days. Addgene: Can you tell us a little more about this new two-color tool and...and see how different parts of the brain or the cells within them work together or perhaps compete against...two-photon properties, so you could activate single cells in arbitrary patterns in the brain. Addgene: Do ...

  11. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...blogger Matteo Tardelli! Matteo Tardelli, PhD is Postdoctoral Scientist at Weill Cornell Medicine in NYC...was contributed by Matteo Tardelli, a postdoctoral scientist at Weill Cornell Medicine. Discussing data...Hamilton DP, Denfeld BA, de Eyto E, Hampton SE, Keller PS, Sharma S, Lewis ASL, Weyhenmeyer GA, O’Reilly...

  12. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...now.  Many thanks to our guest blogger, Kelly Hills! Kelly Hills is a founding bioethicist of the consulting...This post was contributed by Kelly Hills, founding bioethicist of Rogue Bioethics. On November 25, the... the MIT Technology Review dropped a bombshell report. A scientist working in China was using CRISPR/Cas9...separate things happened nearly simultaneously: The Wellcome Trust funded a center for mitochondrial research...consulting firm Rogue Bioethics. Kelly utilizes her firsthand experience in the software industry to inform...

  13. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...multispecies biofilm formed by Pseudomonas spp. and Klebsiella spp. Dr. Devarshi Gajjar, The M. S. University...research focuses on a collection of pathogenic Klebsiella and Pseudomonas isolates causing urinary tract...unknown regulatory regions in Pseudomonas and Klebsiella spp. The effects of these deletions on biofilm...major pathophysiological complications of diabetes mellitus. Recombinant human proteins that play a role in... Researcher who investigates the molecular and cellular etiopathomechanisms of ischemic stroke, the most... reduced blood flow. Novel treatment options, as well as non-pharmacological solutions to modify the course...

  14. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...negative bacteria is due to differences in cell wall composition. Cell wall structure Gram positive bacteria...used in the staining process bleached another, Klebsiella pneumoniae. After realizing this was a repeatable...membrane. Common gram negative bacteria include salmonella and E. coli. Because of their thicker peptidoglycan...complex within the peptidoglycan layer, staining the cell purple. When the alcohol is added, it dehydrates...Silhavy, TJ., Kahne, D. & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2(5) a000414...

  15. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...different types of human cells, including T cells and hematopoietic stem and progenitor cells. Now with the DAP...to enable multiplex gene editing in a variety of cells and organisms. However, lengthy genetic payloads... over 50% averaging editing efficiency in human cells. MBE with DAP array also exhibited reduced Cas9-... editing, and nuclease editing, in virtually any cell type or organisms of interest with only minimal ...

  16. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...of viruses used to deliver CRISPR components to cells or organisms under study. In the past we’ve left... they want to do is to quickly generate a single cell line and proceed to use it for experiments.” - ...screen for genes involved in a wide variety of cellular processes. While these are widely requested and...representation in the viral prep, infect target cells, and, finally, conduct the screen. To help alleviate...of this labor for our users, we now provide the Brunello (targeting human genes) and Brie (targeting mouse...deliver gRNA and therefore, must be used to infect a cell line already expressing Cas9. As John Doench (Broad...

  17. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ... between Cas9 (which causes the breaks) and host cell DNA repair factors (which repair those breaks) makes...example, correcting the allele that causes sickle cell anemia. However, HDR occurs much less frequently...a structure could drive sequence replacement in cells. Targeting three dCas9 molecules to a precisely ...functional Cas9 protein and the targeting guide RNA into cells by electroporation. Results from our lab and others... HDR. The detailed protocol for nucleofection as well as the sequences of our validated editing reagents...Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins...

  18. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ... into the cell. This means that not all neurons and pathways will be transduced equally well by AAV-retrograde...they either trace downstream from the soma (neuron cell body) toward the axon (anterograde tracer) or upstream...them useful tracers, but finding a virus that works well for retrograde tracing can be difficult. The in ... Data Hub!) The cortical pathways to the IC were well transduced by AAVrg while other pathways such as...chemogenetic vector directly into the region where the cell bodies of the projection neurons reside.   Impact...

  19. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...government agencies, active researchers, as well as fellow iGEM teams. We were the proud hosts for the...industrial or research purposes requires stable host cells (the simplest example would be a transformed mesophilic...successful) as host systems since their biology is well studied, and they are convenient to maintain under..., since mesophilic chaperones would not function well under suboptimal conditions. This means recombinant...CRISPR-Cas mediated editing for psychrophilic systems as well as engineering and regulating psychrophilic recombinant...

  20. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...address all your unanswered questions via text! (well, maybe not all of them. We still haven’t gotten ...and would like to get it working in microglia as well. A:  We are not aware of any available AAV for efficiently...co-delivering different AAV particles into the same cells in vivo? A: This will be highly dependent on vector...particularly one that can target the epithelial cells in the blood-brain barrier?   A: For liver, we recommend...worth investigating for both Q6 & Q7.For epithelial cells of the blood brain barrier we would currently recommend...and to all our participants for their time and excellent questions.    Additional Resources on the Addgene...
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