Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 738 results for: HA

Showing: 461 - 480 of 738 results

  2. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...Although at first glance it may appear that there has to be a winner and a loser when negotiating, what... What is most important to each person? Someone has to be the first author but another author can present...

  4. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...CRISPR systems for targeting ncRNAs While CRISPR/Cas9 has proven to be an extremely useful system for modifying...This system is often used to knock down lncRNAs and has fewer off-target effects. Newer Cas protein variants...

  5. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...the tag could be quite small. However, substance P has an amide group added to its C-terminus post-translationally...and Pelham introduced the Myc tag to the world, it has become one of the most commonly used tags in molecular...

  6. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ... long is the average PhD in this department? How has the department worked with students to maintain these... for everyone involved in the process. COVID-19 has changed many aspects of academia, and the PhD application...

  7. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...infect dividing and non-dividing cells.  Addgene has an extensive collection of lentiviral plasmids created... immortalized with adenoviral protein E1A, which has been shown to repress expression of HIV-1 LTRs (more...

  10. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...development for scientists A new plasmid tool your lab has deposited If you’d like to write about a different...

  11. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...protocols, and look - they’re seeking someone who has, to a t, all the research skills under your belt ...their dream job; They want to know if the candidate has what it takes to thrive at the company and add value...

  12. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...? There is a difference. A mentor is someone who has gone where you hope to go, and who will invest in...Joanne Kamens, a strong advocate for career planning, has written a great blog on choosing good mentors for...

  13. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...bloodstream allows for dispersion of the virus but it has to be able to cross the blood-brain barrier to reach...treatments for neurological diseases). The Gradinaru Lab has a lot going on. Here are the key takeaways: Two...

  14. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...requires. Using the (Last name, Year) format also has the benefit of exposing you to relevant researchers...just gotta do it." At the end of the day, someone has to write the article and that someone is going to...

  15. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...invention in the late 1990s, Gateway cloning technology has become very popular as a rapid and highly efficient...attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for many popular...

  17. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...purify DNA, but rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the ...chaotropic salt sodium iodine (NaI) solution. NaI has two purposes in this protocol: 1) it solubilizes ...

  18. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...we should first note that quantitative PCR (qPCR) has been a powerful tool for quantifying AAV for some... 2 minutes and the denaturation time to 1 minute has been shown to increase droplet separation (Witte ...

  20. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...coming directly from grad school. The transition has been a bit rough for her (3 weeks in and there's ...Scientist team says, “a well-run meeting where everyone has a voice and the group can come to a mutual agreement...
Showing: 461 - 480 of 738 results