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  1. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...infect dividing and non-dividing cells.  Addgene has an extensive collection of lentiviral plasmids created... immortalized with adenoviral protein E1A, which has been shown to repress expression of HIV-1 LTRs (more...

  4. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...development for scientists A new plasmid tool your lab has deposited If you’d like to write about a different...

  5. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...protocols, and look - they’re seeking someone who has, to a t, all the research skills under your belt ...their dream job; They want to know if the candidate has what it takes to thrive at the company and add value...

  6. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    Published
    Nov. 14, 2017, 2 p.m.
    ...? There is a difference. A mentor is someone who has gone where you hope to go, and who will invest in...Joanne Kamens, a strong advocate for career planning, has written a great blog on choosing good mentors for...

  7. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...bloodstream allows for dispersion of the virus but it has to be able to cross the blood-brain barrier to reach...treatments for neurological diseases). The Gradinaru Lab has a lot going on. Here are the key takeaways: Two...

  9. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ...coming directly from grad school. The transition has been a bit rough for her (3 weeks in and there's ...Scientist team says, “a well-run meeting where everyone has a voice and the group can come to a mutual agreement...

  10. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...native organism.” This is possibly a better idea and has been used successfully in the past (Angov et al.,...functionally analyze the protein produced to ensure that it has folded properly. You may be able to avoid getting...

  11. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...requires. Using the (Last name, Year) format also has the benefit of exposing you to relevant researchers...just gotta do it." At the end of the day, someone has to write the article and that someone is going to...

  12. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...invention in the late 1990s, Gateway cloning technology has become very popular as a rapid and highly efficient...attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for many popular...

  14. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...purify DNA, but rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the ...chaotropic salt sodium iodine (NaI) solution. NaI has two purposes in this protocol: 1) it solubilizes ...

  15. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...we should first note that quantitative PCR (qPCR) has been a powerful tool for quantifying AAV for some... 2 minutes and the denaturation time to 1 minute has been shown to increase droplet separation (Witte ...

  16. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and rAb plasmids...plant, or mammalian cells. Each of these systems has their advantages and disadvantages. Briefly, bacterial...

  17. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...University of Miami. The beginning of this century has seen some major advances in light microscopy, particularly...unprecedented detail.  One of these advances in microscopy has been light sheet fluorescence microscopy (LSFM). ...

  19. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...transcript, you will also need to make sure your plasmid has a promoter suited to working in your host organism... but requires SP6 RNA polymerase. SP6 polymerase has a high processivity. When used for in vitro transcription...
Showing: 461 - 480 of 727 results