We narrowed to 743 results for: HA

...Although at first glance it may appear that there has to be a winner and a loser when negotiating, what... What is most important to each person? Someone has to be the first author but another author can present...

...useful in an experiment, and want to see if someone has already created and deposited it with Addgene. Combining...resistance gene(s) used for selection in bacteria. Has Viral Service?: Allows you to filter results to only...

... of stem cell potency: Totipotency: When a cell has the ability to produce all of the differentiated ... are totipotent [2-4]. Pluripotency: When a cell has the ability to differentiate into cells of any of...

... to edit genes in several biological systems. It has been used for instance in inherited disorders such... is often used, the CRISPR approach based on HDR has the advantage of being a potentially universal treatment...

...CRISPR systems for targeting ncRNAs While CRISPR/Cas9 has proven to be an extremely useful system for modifying...This system is often used to knock down lncRNAs and has fewer off-target effects. Newer Cas protein variants...

...the tag could be quite small. However, substance P has an amide group added to its C-terminus post-translationally...and Pelham introduced the Myc tag to the world, it has become one of the most commonly used tags in molecular...

... long is the average PhD in this department? How has the department worked with students to maintain these... for everyone involved in the process. COVID-19 has changed many aspects of academia, and the PhD application...

...transsynaptic spread is useful for neuronal tracing, it has two practical limitations. First, these high levels...projection neurons). Research into these phenomenon has shown that the RABV G protein is necessary and sufficient...

...infect dividing and non-dividing cells.  Addgene has an extensive collection of lentiviral plasmids created... immortalized with adenoviral protein E1A, which has been shown to repress expression of HIV-1 LTRs (more...

... started from 474 plasmid deposits in 2014 which has grown to ~10,000 plasmid deposits per year in recent...

...badge in the image below. If an abstract on PubMed has the “Find Plasmids” badge, you’ll know that within...

...development for scientists A new plasmid tool your lab has deposited If you’d like to write about a different...

...for bimolecular fluorescence complementation BiFC has been used to directly visualize PPIs that regulate... PIPs in which the protein-interaction interface has been compromised (Kodama and Hu, 2012). Such controls...

...purify DNA, but rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the ...chaotropic salt sodium iodine (NaI) solution. NaI has two purposes in this protocol: 1) it solubilizes ...

...we should first note that quantitative PCR (qPCR) has been a powerful tool for quantifying AAV for some... 2 minutes and the denaturation time to 1 minute has been shown to increase droplet separation (Witte ...

...deactivate specific genes are needed. Targeted mutation has been demonstrated in zebrafish using zinc-finger ... in all cells of the developing zebrafish embryo has been mapped using single cell RNA sequencing approaches...

...native organism.” This is possibly a better idea and has been used successfully in the past (Angov et al.,...functionally analyze the protein produced to ensure that it has folded properly. You may be able to avoid getting...

...coming directly from grad school. The transition has been a bit rough for her (3 weeks in and there's ...Scientist team says, “a well-run meeting where everyone has a voice and the group can come to a mutual agreement...

...bloodstream allows for dispersion of the virus but it has to be able to cross the blood-brain barrier to reach...treatments for neurological diseases). The Gradinaru Lab has a lot going on. Here are the key takeaways: Two...

...sample with a good A260/280 but a low A260/230 likely has some residual guanidine thiocyanate. Phenol is a ...contamination — just be sure to check that your RNase has not expired (or been forgotten).    Improving read...