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We narrowed to 889 results for: IND

Showing: 461 - 480 of 889 results

  1. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...nucleic acid sequences that bind to the small molecule, DFHBI-1T to induce its fluorescence. They found...protein (dCas9) is well known for its ability to bind DNA targets without changing them. Thus, it has ...genomic loci brightly, give CRISPR-Sirius a try. Find CRISPR-Sirius plasmids at Addgene. References Ma...Addgene.org Browse our CRISPR plasmids for visualization Find plasmids for your research Read our CRISPR guide...

  2. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ... proteins (FPs) have become one of the most indispensable tools in modern biological research.  Any microscopist...proteins when missing, or edit inaccuracies as they find them (anyone can register with a valid email address...FPbase The most obvious use case for FPbase is finding information on a particular FP; this can be done...600nm?  Simply input these parameters and you’ll find matching FP suggestions. If you’re not sure exactly...Addgene.org Browse all fluorescent protein plasmids Find plasmids for FRET Check out the Michael Davidson...

  3. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    Published
    Aug. 23, 2022, 1:15 p.m.
    ... but with the names reversed, meaning that the findings of the study were also reversed. The third group...participants read an abstract for a study with findings that challenged their beliefs, they were much ...catchall term for a range of neat little tricks our minds perform to avoid cognitive dissonance (Kunda, 1990...gained from that research remains inaccessible, behind paywalls and in technical jargon that even scientists...References Drummond, C., & Fischhoff, B. (2017). Individuals with greater science literacy and education have...

  4. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...strategy that would independently generate multiple gRNAs for nCas9-BE, or any other kinds of CRISPR tools...expression of the array. After expression, the individual gRNAs are released by endogenous tRNA processing...which is followed by tRNA processing to release individual gRNAs for gene editing. They named this new array...-target editing and do not cause higher Cas9-independent off-target editing in comparison with single-...

  5. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    Published
    Feb. 20, 2018, 2:30 p.m.
    ...Libraries Resources at Addgene.org Find pooled libraries at Addgene Find basic lab protocols at Addgene ...preparing DNA and generating/replenishing kits, is to find new ways to make lab processes more efficient. Being... now know what to expect at a workshop and what kinds of questions to ask myself when I am bombarded with...information I’ll be learning relate to me as an individual and Addgene as a company? What’s the key thing...

  6. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...shift can be prevented. Keep this information in mind if you’re planning on shipping something on dry ...medium, which is buffered and contains the pH indicator phenol red. We froze samples in a -80°C freezer... the samples were at the control pH of 7.4 as indicated by color (data not shown). What we did next: Venting... at -80°C. We then thawed the tubes after the indicated incubation periods, and measured the pH. We found...Resources on Addgene.org Check out the Viral Service Find Virus Protocols Browse Our Viral Vectors ...

  7. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

    Type
    Blog Post
    Published
    June 1, 2021, 1:15 p.m.
    ...collaborator for a specific task, Crowdfight will: 1) Find an expert with the right skill; 2) Confirm the expert...been essential to push certain projects. The idea behind the platform was simple: any person working on ... collaborations are limited by the difficulty to find the right expert and the lack of credit for small...researchers who believe in making science less of an individual effort and more of a collective construction....research. It’s completely free, of course. You can find more information about us and what we do in our ...

  8. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...opportunities available to those who are scientifically minded! Listen to Biotech Founder Michael Koeris' Journey...in-depth research is applicable to a wide range of industries and is a highly desirable quality sought after... are self organised, reliable, and can work independently. Practical outdoor skills are needed in a variety...currently in the position. Questions you’ll want to find answers to include: Will you be sitting at a desk... posts for more specifics on some careers and to find advice on crafting the perfect application. Additional...

  9. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...agile framework If you can’t find internships at science companies, try to find internships with translatable...relationship-building in order to rally many stakeholders behind a common vision. Use your network to your advantage... out the infographic for an outline of my day or find the text version below. 8:00 AM--Log on! My team...software and technology tools with the customer in mind. You can follow her on twitter @rgrubinstein. Additional...

  10. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...milestone in a scientist's career is mediated by some kind of communication to another person.  In an interview...specialized franchise workshops throughout the year.  Find workshops in your area here: https://comscicon.com...out of our workshops. If you're having trouble finding science communication opportunities in your area...do, let us know!  To get in touch with ComSciCon, find us on Twitter, Facebook, Instragram, or send an ...works as a data scientist in the entertainment industry.       Additional resources on the Addgene Blog...

  11. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...regulation is complex and influences--directly or indirectly--many other areas of biological research. If ...field who have deposited with Addgene, but you’ll find many great tools by clicking on the links below ...below pull up lists of plasmids containing the indicated gene, and you can subscribe to these pages to ...to pages containing lists of plasmids from the indicated publications. You can request all of these plasmids...956-972.e23 Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after...

  12. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...common antimicrobial agent added to buffers, can bind to amine groups present in some conjugates, blocking...conjugates, or signaling molecules! Please keep in mind that this is just a brief introduction to the wide... if it ain’t broke, don’t fix it. HRP is easy to find, simple to use, not sensitive to light in its inactive.... But PE can be very helpful if you're trying to find a relatively rare protein in an experiment with ...Introduction to Antibodies Resources on Addgene.org Find plasmids encoding recombinant antibodies at Addgene...

  13. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ... to find protocols that require reagents which aren’t broadly or easily available, so you may find yourself...learn more about your protein of interest, you may find the available amino acids a bit…limiting. It may...type, and growth temperature) may need to be individually optimized for each of the three factors: the...trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact both ...

  14. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...can begin to understand how the brain works. It’s kind of like “the head bone’s connected to the neck bone...information or tools, making them useful tracers, but finding a virus that works well for retrograde tracing ...serotype in both mice and guinea pigs (work you can find in AAV Data Hub!) The cortical pathways to the IC...Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service Resources...Resources on Addgene.org Visit Our AAV Guide Page Find Ready-to-use Virus for Your Research Visit Our Lentivirus...

  15. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ... plasmids can induce gene-specific silencing temporarily, long-term, and even inducibly depending on the...expression cassettes typically contain a promoter (inducible or constitutive), your gene of interest, and often...Screening libraries, large collections of biological independent variables, are becoming increasingly popular ...or maybe first!) viral experiment? Click Here to Find Viral Vector Resources at Addgene! References and...

  16. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral prep here Control AAV Control...presence of GFP to form a functional Cre enzyme that induces recombination of lox sites. This system takes advantage...Addgene blog Read all of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors...

  17. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ... Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization...are converted into emitted photons. These are indicated by the extinction coefficient (EC) and quantum... variation in the fluorescence intensities of individual mammalian cells within the dish. This is caused... brightness can be made (Goedhart et al, 2012, Bindels et al, 2017). The plasmid for co-expressing mTurquoise2...

  18. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...Service Resources on Addgene.org Find Ready-to-Use Lentivirus Find Ready-to-Use AAV Catch up on Chemogenetics...Viral Service (and then we crunched them again) to find the most requested lentivirus and AAV of 2016. The... (using pooled CRISPR knockout libraries) and individual gene knockouts (using gRNA lentiviruses). Separating...AAV pAAV-hSyn-DIO-hM4D(Gi)-mCherry can be used to induce neuronal silencing in vitro and in vivo (Krashes...

  19. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...straightforward, but plasmid-based SDM has lagged behind. While commercial kits are available for making...GeneArt cloning - but at a much lower cost. Read on to find out how to replace your SDM method with REPLACR ...some of these methods, chances are you’d like to find a better one. Trehan et al. wondered if, instead... in which the phage recombination machinery is induced using L-arabinose. These cells can be made and ...Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research ...

  20. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    Published
    July 28, 2015, 2:30 p.m.
    .... ChIP-seq relies on antibodies to recognize and bind transcription factors directly, but that’s a slow-going... transcription factors and figure out where they bind to the genome. We’ve been doing that for years using...actually use this for tagging any protein in all kinds of different assays, not just transcription factors...two-fold: CRISPR/Cas9 offers high efficiency of inducing double strand breaks and more opportunity for ...Professors Mendenhall and Myers for speaking with us! Find the Plasmids: Plasmids for protein tagging with...
Showing: 461 - 480 of 889 results