We narrowed to 616 results for: LIS;

1. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

   Type

   Blog Post

   Published

   July 18, 2017, 12:41 p.m.

   ...Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen to Our Interview with Previous Awardee Kwabena ...

2. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

   Type

   Blog Post

   Published

   Aug. 22, 2019, 12:43 p.m.

   ...comes from the gram-positive bacteria Bacillus subtilis and encodes the enzyme levansucrase that converts...

3. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

   Type

   Blog Post

   Published

   June 11, 2020, 1:34 p.m.

   ... This response is immediate, on the scale of milliseconds, and can precisely link a neuron's activation...

4. Plasmids 101: What is a plasmid?

   Type

   Blog Post

   Published

   April 2, 2020, 6:17 p.m.

   ...Originally published Jan. 14, 2014 and updated Apr. 14, 2020. Any newcomer who joins a molecular biology...

5. Antibodies 101: Fc Effector Functions

   Type

   Blog Post

   Published

   May 18, 2023, 1:15 p.m.

   ...don’t generally affect antibody applications like ELISAs or Western blots, they are key in helping antibodies...

6. Hot Plasmids - October 2022

   Type

   Blog Post

   Published

   Oct. 6, 2022, 1:15 p.m.

   ...!) The Myc-tag is a string of 10 amino acids, EQKLISEEDL, that can be incorporated into your protein of...

7. Understanding GPCR Signaling Using TRUPATH

   Type

   Blog Post

   Published

   Dec. 14, 2020, 2:15 p.m.

   ...the blog post. References Olsen RHJ, DiBerto JF, English JG, Glaudin AM, Krumm BE, Slocum ST, Che T, Gavin...

8. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   Published

   March 15, 2018, 12:59 p.m.

   ...different strands to create a DSB, but this can be accomplished with either a PAM-in or PAM-out orientation....

9. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

   Type

   Blog Post

   Published

   Feb. 13, 2018, 2:51 p.m.

   ... your own lab Very recently, the Henikoff lab published a preprint paper outlining improvements to the...

10. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type

    Blog Post

    Published

    Oct. 3, 2017, 12:11 p.m.

    ...], so making it a success feels like a real accomplishment.” He started working on them shortly after ...

11. New and Upcoming Viral Vectors - Spring 2019

    Type

    Blog Post

    Published

    March 26, 2019, 1:40 p.m.

    ...Addgene. Viral vectors coming soon! These vectors listed below should be released at Addgene in the next...

12. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type

    Blog Post

    Published

    Dec. 13, 2018, 1:14 p.m.

    ...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

13. Plasmids 101: Origin of Replication

    Type

    Blog Post

    Published

    Nov. 11, 2020, 2:23 p.m.

    ...Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic...

14. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type

    Blog Post

    Published

    March 5, 2020, 2:15 p.m.

    ...which is equally important to report. We recently published a paper in Frontiers in Neuroanatomy and summarized...

15. Summer SciComm: Science Storytelling

    Type

    Blog Post

    Published

    Sept. 16, 2022, 1:15 p.m.

    ...attention enough to make them decide to read (or listen or watch) further.  Second, the introduction needs...

16. Antibodies 101: Epitope Tags

    Type

    Blog Post

    Published

    May 11, 2023, 1:15 p.m.

    ... Myc The Myc tag is a 10 amino acid peptide (EQKLISEEDL) derived from the protooncogene of the same name...

17. Antibodies 101: Chimeric Antibodies

    Type

    Blog Post

    Published

    April 25, 2023, 1:15 p.m.

    ...Protein B. The antibodies are both extensively published, knockout validated, and work in your application...

18. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type

    Blog Post

    Published

    Aug. 18, 2023, 4:15 p.m.

    ...Interactions (pp. 23–40). Springer International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 ...

19. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type

    Blog Post

    Published

    Nov. 12, 2019, 2 p.m.

    ...nts, and is thermostable between 31-100°C. They published their finding in a bioRxiv article and in PNAS...

20. CRISPR Challenges: Standardization and Homology Directed Repair

    Type

    Blog Post

    Published

    Feb. 14, 2018, 2:36 p.m.

    ...Addgene) don’t forget this in the future. Here’s a list of blog posts to help you tackle the challenges ...