We narrowed to 499 results for: SHA
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TypeBlog PostPublishedMay 21, 2024, 1:30 p.m....briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based: if ...
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Plasmids 101: Broad Host Range Plasmids
TypeBlog PostPublishedApril 11, 2024, 1:15 p.m....Jain and Srivastava 2013). They are also easily-shareable tools within the scientific community, as they... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m....and loxP sites differ at the nucleotide level but share an overall structure of 13 base pair palindromic... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....population, and it is unclear how this will all shake out. In the short term, postdocs and potential postdocs... -
The Challenges of Cell Culture
TypeBlog PostPublishedMay 5, 2016, 2:30 p.m....majority of cell line problems come from people sharing cells: the black market of cell biology. Engaging... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....research benefits when the advances of some are shared with all. Due to the versatility and standardization... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog PostPublishedFeb. 10, 2015, 3:06 p.m....reducing the temperature or moving it to a bench-top shaker 10-20 minutes before adding the inducer. Growth... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m....23287718. PubMed Central PMCID: PMC3795411. 2. Mali, Prashant, et al. "RNA-guided human genome engineering via... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog PostPublishedMay 5, 2020, 1:15 p.m....Azhar, M., Phutela, R., Ansari, A. H., Sinha, D., Sharma, N., Kumar, M., … Maiti, S. (2020). Rapid, field-deployable... -
Adeno-associated Viruses (AAVs) for Genome Editing
TypeBlog PostPublishedMarch 27, 2018, 1:32 p.m..... PubMed Central PMCID: PMC4752446. 5. Asuri, Prashanth, et al. "Directed evolution of adeno-associated... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m....more about antibodies Read about another reagent sharing platform for antibodies, the Developmental Studies... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog PostPublishedJune 17, 2025, 1:15 p.m....pri-miRNA, which is then processed by a complex called Drosha to pre-miRNA. miRNA plasmids are engineered to ... -
Hot Plasmids: Summer 2024
TypeBlog PostPublishedAug. 27, 2024, 1:15 p.m....find a recombinant anti-GFAP [N206A/8R] antibody shared by James Trimmer’s lab (Figure 5) as well as antibodies... -
Tips for Getting a Faculty Position
TypeBlog PostPublishedJuly 20, 2023, 1:15 p.m....positions in biomedical science. My motivation was to share my experiences from my own job search and my time... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog PostPublishedDec. 6, 2022, 2:15 p.m.... vivo use and deep-tissue imaging. Vladislav Verkhusha’s lab has generated a new genetically encoded calcium... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog PostPublishedOct. 11, 2016, 2:30 p.m....doi.org/10.1371/journal.pone.0154027 Wang Y, Cheng X, Shan Q, Zhang Y, Liu J, Gao C, Qiu J-L (2014) Simultaneous... -
Site Directed Mutagenesis by PCR
TypeBlog PostPublishedAug. 2, 2016, 2:30 p.m....PMC323600. 2. Chester, Nicholas, and Daniel R. Marshak. "Dimethyl sulfoxide-mediated primer Tm reduction... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog PostPublishedJune 23, 2016, 2:30 p.m....: 1151-1154. PubMed PMID: 21868676. 6. Pirman, Natasha L., et al. "A flexible codon in genomically recoded... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog PostPublishedNov. 25, 2015, 4 p.m.... 25590106. Paula Stephan’s Book - How Economics Shapes Science The Future of Research Website Additional...