...endogenous function of the gene as a result but will still allow for expression pattern monitoring. Enhancer...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...insertion into an exon, like promoter traps, it will still likely disrupt the gene it monitors due to transcriptional...

...conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled...of the Rluc8 insertion within the Gα. Despite exhaustive refinement, it was not possible to generate an...a target of many pharmaceutical drugs, there is still only a partial understanding of how GPCRs signal...

...content that scientists will find useful and interesting than that will simply get people ordering plasmids...analytical thinking, time management, information distillation, etc) are transferable to many different fields...thinking about preparing yourself for a career while still in graduate school, you’ll be well suited to follow...

...feedback in formal sessions using a specific list of questions after a task or period of work. Using this standard...For a formalized feedback session, answer the questions below together and discuss openly. Both parties...helped if the feedback session is over and they still don’t know how you actually feel about their performance...

... my job I most enjoy is building and telling interesting stories — whether I’m working with a talented...storytelling to answer an informational interview question: Interviewer: “Tell me about a time you had a ...your website up to date, invite people to ask questions, be approachable. Engage with your audience and...

...to stay and good reasons to go.  Here are some questions and perspectives to consider. What do academic...have in common? You can do quality science on interesting topics Having strong non-science skills is important...important for success Must always justify use of resources and pay attention to budget constraints Academic...

...are incredibly useful, chemical fluorophores can still be used in a wide variety of experiments. Read more...and Alexander Green (both formerly at the Wyss Institute) to learn how these amazing tools may be used ...through Addgene.  Thanks again to all of our fantastic depositors and guest writers. Your contributions...

...of the Ocular Genomics Institute, and an Associate Member at the Broad Institute of Harvard and MIT. ... of Oncology Target Discovery at the Novartis Institutes for Biomedical Research. Drug development was...

...localize opsins to the soma to avoid off target photostimulation of axons and dendrites on neighboring neurons... AAV in smaller aliquots of 20 ul for serotype testing to help you pick the best AAV for your experiment...control plasmids in new serotypes. See our Serotype Testing AAV inventory for the entire collection! Plasmid...

...addgene.org to learn more or get started.  If you have suggestions about how to make the resource better or ideas...collection, please let us know by filling out this suggestion form. As we build the collection, we hope that... represent the official views of the National Institutes of Health....

...Alejandro Balazs, a Group Leader at the Ragon Institute of MGH, MIT, and Harvard, and his lab found that...SARS-CoV. These two viruses are quite genetically distinct from SARS-CoV-2 and its variants. Yet, the B.1.351... Cele S, Gazy I, Jackson L, Hwa S-H, Tegally H, Lustig G, Giandhari J, Pillay S, Wilkinson E, Naidoo Y...

...nucleic acids? Sounds sort of dangerous at first suggestion! The good news is that viruses are very valuable...long-term? Expressed short-term? Answering these questions will help guide you to the right method for your...are non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform...

...encode biosynthetic enzymes for violacein, a distinctive pigmented protein. By altering the transcriptional... By using an orthogonal dCas9 protein that will still recognize guide RNA but with a different dCas9 binding...of transcriptional programs can be layered with distinct programs controlled by each dCas9 regulatory switch...

...Oliver Griesbeck of the Max Planck Institute for Neurobiology has been working on genetically encoded...these Twitch sensor variants were optimized by testing various linker configurations in a large-scale ... is disrupted in the presence of a ligand (they stick together in one state, but not the other). “It’s...

...for making your meetings productive: Discuss logistics if appropriate:  How often will you meet? How ...smaller, actionable steps (e.g. Meet 10 PhDs with interesting careers, find a summer internship, etc.). It ...Check Out Angela Depace's Talk on "Precision and Plasticity in Mentoring"    ...

... Which graduate student hasn’t been asked the question: “Academia or industry?” Once academia was the...opportunities beyond academia. Once a profession sounds interesting, TAKE ACTION! Taking action: Examples of extracurricular...'d like to learn more about next. Leave your suggestions in the comments section.  ...

... by an anterograde AAV was shown to drop off drastically with lower titers. As you can see in figure 2...many more viral particles to your solution while still using a volume that’s compatible with your experimental...fewer opportunities your viral particles have to stick to your tubes, the higher titer you will have. When...

...companies and BosLab has grown into an organization consisting of over twenty members and volunteers. Transforming... prior to getting started. For example, Timothy Stiles joined BosLab last year with a computer science...developed community projects. Recently, Timothy Stiles and Mrina Nikrad have started the Open Yeast Engineering...

...avenues previously unavailable to them. With these fantastic tools it’s now easy to monitor protein expression...loops on either side of an enzyme’s active site and still block enzyme function upon exposure to ultraviolet...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter...

...ug/ml pure dsDNA. Use the following formula to estimate your DNA: Concentration (ug/ml) = A260 reading...sensitivity at low concentrations of DNA and it can’t distinguish between DNA and RNA. See our protocol about using...dyes that fluoresce when bound to DNA. The main distinction here is that these dyes, such as PicoGreen or...