We narrowed to 608 results for: Sis;

1. Advice for Choosing a Research Project

   Type

   Blog Post

   Published

   April 17, 2014, 2:45 p.m.

   ...to use to reach these milestones? Can you get assistance from people who have the required expertise to...

2. The Stingy Scientist: How the Baby Gel Box Was Born

   Type

   Blog Post

   Published

   April 15, 2014, 3:11 p.m.

   ...before the advent of techniques like PCR and DNA synthesis. Most molecular biologists bought the pricey stuff...

3. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

   Type

   Blog Post

   Published

   June 2, 2020, 1:15 p.m.

   ...designed a calcineurin transcriptional reporter consisting of a quickly-degraded destabilized GFP variant...

4. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

   Type

   Blog Post

   Published

   Oct. 10, 2019, 12:41 p.m.

   ...Central PMCID: PMC427010. Porter, R. (1959). The hydrolysis of rabbit y-globulin and antibodies with crystalline...

5. Hot Plasmids and Viral Preps - September 2021

   Type

   Blog Post

   Published

   Sept. 21, 2021, 1:15 p.m.

   ...selection marker, Thy1.1, in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide...

6. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

   Type

   Blog Post

   Published

   June 22, 2021, 1:15 p.m.

   ...The lab saw that the B.1.351 variant was very resistant to vaccine serum responses. This result was similar...

7. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

   Type

   Blog Post

   Published

   May 25, 2021, 2:29 p.m.

   ...neuroscience research. More broadly, advances in gene synthesis, affinity reagent screening, and next-generation...

8. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

   Type

   Blog Post

   Published

   June 27, 2018, 1:16 p.m.

   ...fluorophore, or APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP...

9. Viral Vectors 101: Transductions

   Type

   Blog Post

   Published

   April 30, 2024, 1:15 p.m.

   ...non-integrating. Read on to find out more! Molecular basis of transduction Transductions utilize the ‘normal...

10. What the HEK?

    Type

    Blog Post

    Published

    April 18, 2023, 1:15 p.m.

    ...many immature neuron-like qualities, which is inconsistent with kidney cells (Shaw et al.). Since then,...

11. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type

    Blog Post

    Published

    Sept. 15, 2015, 2:30 p.m.

    ...AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied...

12. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    Published

    July 7, 2015, 3:36 p.m.

    ...selection cassette containing genes for hygromycin resistance and the Roller movement phenotype. To make these...

13. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type

    Blog Post

    Published

    April 7, 2015, 4:21 p.m.

    ...control The CRISPR interference (CRISPRi) model consists of a catalytically dead Cas9 protein (dCas9) that...

14. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type

    Blog Post

    Published

    March 4, 2014, 4:49 p.m.

    ...became aggressive. If we can understand the neural basis of violence or aggression, it might do a lot to ...

15. 25 Work From Home Tips for Scientists

    Type

    Blog Post

    Published

    March 19, 2020, 1:15 p.m.

    ...communities are looking for bench scientists to assist in labs that are testing for COVID-19. You can ...

16. Celebrate National Book Month with Addgene

    Type

    Blog Post

    Published

    Oct. 3, 2019, 12:47 p.m.

    ...plants, among others. I particularly enjoyed the emphasis that microbes are neither bad or good - they're...

17. FPbase: A new community-editable fluorescent protein database

    Type

    Blog Post

    Published

    May 16, 2018, 1 p.m.

    ...lineages, 2-photon spectra, more elaborate spectrum analysis tools, and a better way to store and represent...

18. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    Published

    Sept. 19, 2017, 1:20 p.m.

    ...., Vitriol, E., & Hahn, K. (2008). Chromophore-assisted laser inactivation in cell biology. Trends in ...

19. Pushing the Limits of DNA Assembly

    Type

    Blog Post

    Published

    Sept. 6, 2017, 1:01 p.m.

    ...HiFi can simplify multi-position site-directed mutagenesis by assembling multiple fragments containing the...

20. Visualizing Translation at the Single Molecule Level

    Type

    Blog Post

    Published

    Aug. 1, 2017, 1:15 p.m.

    ...References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis in vivo of translation with nucleotide resolution...