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  1. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ... interest to create a physical map of the DNA. Starting in the 1970s, restriction mapping was commonly...

  4. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark GR (1979) Transfer of proteins from gels to diazobenzyloxymethyl-paper...

  6. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...well. Quickly after transfection (4-8h), cells start releasing viral particles into the culture supernatant...

  12. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...interpret flow cytometry data users will typically start by creating a dot plot of FSC versus SSC that allows...

  15. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...construct. DNA and gRNA are not to scale.   Let’s start with the simplest multiplexing situation: you only...

  17. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...plasmids here Check out the Yamanaka paper that started the field of iPSCs Find gene-specific plasmids ...

  18. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...in protein expression. (If you're just getting started, check out our Introduction to PCR.) But if you're...

  19. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...replace entire protein-coding regions (excluding start and stop codons) with kanamycin cassettes and were...

  20. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...mechanisms. Rare observations like these may be a starting point for future developments in the field of ...
Showing: 461 - 480 of 482 results