...signaling pathways. Rho guanine-nucleotide exchange factors (GEFs) activate their partner Rho GTPase, leading...to reveal even more insights. Super-duper lipid editor And if recruiting proteins to the cell membrane...phosphatidic acid signaling (such as Hippo, AMPK and mTOR pathways) and to introduce unnatural head groups...membrane or lysosomes and its activity can be monitored by phosphatidic acid probe GFP-PASS. B) Time series...Additional resources on the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org...Activity-based directed evolution of a membrane editor in mammalian cells. Nature Chemistry, 15(7), 7....

...list from your current P.I., lab manager, or administrator containing all of the materials that you’ve ...financial values. All too often, we see early investigators making unnecessary short term sacrifices that...negotiations can still go horribly awry when several factors aren’t taken into consideration. 1. Always focus...solutions to reach your goals. For example, a junior investigator turned down a faculty position because none ...Happy Sciencing, Damien   Damien Wilpitz is a laboratory research management consultant and coach in Boston...research scientists create productive and manageable laboratory environments. His business skills enhance his...

...construct (about 8 in Brainbow-1.0 mice.) The combinatorial effect of multiple, independent recombination...woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), commonly used to increase transgene...Addgene also has two Brainbow adeno-associated viral vectors (AAV) available - AAV-EF1a-BbChT and AAV-EF1a-BbTagBY...of neural circuits, such as connections between motor neurons and the neuromuscular junction. In zebrafish...plasmids References: Transgenic strategies for combinatorial expression of fluorescent proteins in the nervous...PubMed. Multiplex cell and lineage tracking with combinatorial labels. Loulier K, Barry R, Mahou P, Le Franc...

...combining AcrIIA4, a Streptococcus pyogenes Cas9 inhibitor, with the LOV2 photosensor from Avena sativa, ...-CRISPR plasmids Tagging human transcription factors with GFP Kevin White’s lab recently deposited a... of CRISPR plasmids to tag human transcription factors with GFP. As part of the Encyclopedia of DNA Elements...plasmids will help researchers map transcription factor localization and binding via ChiP-seq. Learn ...Learn more about ENCODE Find the transcription factor tagging plasmids If you have a new CRISPR tool you...

...Addgene with our Executive Director, Joanne Kamens. As a regular contributor of careers blog articles, ... our visitors made sure to tell us what we already knew about our Shipping Operations Coordinator: “Kory...teams at Addgene. An introduction to plasmids with Director of Biology Lianna Swanson was very popular, with...

... take into account chromatin context and other factors, they are easy to use and can reduce off-targets...nicking/cleaving of DNA occurs, not for deadCas activator/repressor experiments where only localization ...- GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ pathway...RNA-seq would be an appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would... (e.g. in a gene in a similar pathway, a growth factor, etc.) this control is usually not very meaningful...and off-target risks will vary depending on many factors. Hopefully now you have all the resources you need...

...and luckily genetics is a sector which has been rapidly growing. These factors left me optimistic that ...and the pro’s and con’s of working in different sectors. Luckily, I have worked for a variety of different...options for going into parasitology but another major factor held me back; I do not have a second language. ...allowed me to try out working in many different sectors and to discover for myself what potential job opportunities... Are Open at Addgene Learn How to Choose a Good Mentor for Your Professional Development ...

...Chinmaya Sadangi, a postdoc at the University of Toronto. The Addictive Brain was founded in early 2018 ...Chinmaya Sadangi, a postdoc at the University of Toronto, created The Addictive Brain to spread awareness...non-scientists can directly approach scientists and doctors with their questions and comments.  Lastly, we ...Keep your message to the main three points of your story. Interested in learning more? Visit their website...Sadangi is currently a postdoc at the University of Toronto and founder of The Addictive Brain. Prior to this...

...signaling activity and cell phenotype. One method for monitoring live single-cell kinase activity is FRET, but... distance and the orientation of the donor and acceptor proteins, which can be difficult to predict. ...disadvantages when compared to FRET reporters. First, KTRs monitor global kinase activity that occurs in the cytoplasm...experiments. Third, KTRs are not well suited for monitoring kinase activity that occurs during nuclear envelope...the nucleus, there’s no nuclear localization to monitor). Whether you’re looking for a ready-to-use KTRs...

...here!   Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School ...Cas9) hematopoietic stem cells with a lentiviral vector containing a gRNA. These transduced stem cells ...custom mouse strains and lentiviral expression vectors (Table 1). The four systems Name Function...G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible, lineage-specific and sequential ...

...The first is the study of non-coding regulatory elements. Regulatory DNA function is typically inferred ...interested in exploring the function of non-coding regulatory elements in their native chromatin context. An...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical...PhD student at Harvard Medical School in the laboratories of Stuart Orkin and Daniel Bauer. He received...

... successful, internationally renowned plasmid repository that it is today. Although I wish I could take... attributed to the entire Addgene family; the depositors and requesting scientists, all of the Addgene..., Addgene supporters, and of course, Executive Director, Joanne Kamens, and founders, Melina Fan, Ken ...about her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used...Joanne shared a moving thank you email from a requestor in Iraq (Addgene's first). After the speeches,...

...wanted users to be able to find plasmids and viral vector tools more easily. In the redesign, the new search...unique: community, sharing, and fun. The Addgene repository is built on the foundation of a community that...dictate the space. We wanted to comfortably tell our story, to get scientists excited about what Addgene has...redesigning the homepage, and striving to tell our story in better ways, we realized that Blugene needed ...you come to Addgene to find the plasmid or viral vector for your next experiment, that you can find what...

...  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs at UNC, called her presentation...current postdocs will be academic principal investigators. She also pointed out promising new practices...increasing the value and priority put on good mentoring and applauded the new requirement for PIs to include...opened with a discussion of the Novartis industry postdoctoral program.  His strongest comments were about ...

... markers all tagged with mEmerald. Plasmids to monitor protein-protein interactions through multicolor... Baird said. “They are all designed in the same vector backbone with very similar restriction enzymes....Michael Davidson told the Tallahassee Democrat in a story about his life’s work and the stage IV lung cancer...shutter its doors. “We wanted to give all of these vectors to the scientific community and contribute in a...community," says Joanne Kamens, Addgene's Executive Director, "and to promote the great work of Michael Davidson...

...technologies, one might incorrectly relegate RNAi to history. The straightforwardness of RNAi is unmatched—unlike...cannot be used to investigate essential genes. Historically, gene silencing methods were thought to be afflicted...considerations, such as the starting material (DNA-based vector or purified RNA) and the initial state of the dsRNA... Although downstream work is required, several vectors can be found at Addgene that could serve as an ...an excellent start (#22988, #137884). Both vectors allow the creation of amiRNAs based upon miRNA precursors...method. Instead of using a fluorescent output to monitor RNAi, we engineered a positive selection reporter...

...chance vs. the factor that is being experimentally manipulated. Below are several core factors of statistics...variables – factors which are not affected by other variables (cause). Dependent variables – factors which...total crop production and the production window. Factorial ANOVA This type of ANOVA assesses multiple independent...sounds like a two-way ANOVA. And this time, it is! Factorial ANOVAs include two-way ANOVAs but can include ...

...of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting... autophagic activity, and are a more reliable indicator of autophagic activity. The aim of this post is...mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It’s important to remember that because...Maria Cuervo, and Per O. Seglen. "Methods for monitoring autophagy from yeast to human." Autophagy 3.3...PMC4517063. Yoshii, Saori R., and Noboru Mizushima. "Monitoring and measuring autophagy." International journal...

...Willoughby   Using RUBY as a selection marker The creators of RUBY highlight its use in crop plants under... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...promoter with self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al...., Sun, H. et al. A reporter for noninvasively monitoring gene expression and plant transformation. Hortic...Transformation CRISPR 101: CRISPR-Mediated Plant Base Editors...

...model for cancer-specific research. Membrane receptors and ion channels are often exogenously expressed... degrade over time in culture. This can affect factors that could skew research results (gene expression... Especially if your research interests include receptor biology, ion channels, or involve human protein...Platform to Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI...10 Tips for Mammalian Cell Culture Adenoviral Vector Production and Troubleshooting Additional resources...