Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 719 results for: Tat

Showing: 461 - 480 of 719 results

  2. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...However, the first two generations of iGluSnFR had limitations: they were not optimal for experiments requiring...photon imaging.   Figure 1: 1P and 2P excitation and emission spectra of SF-Venus-iGluSnFR.A184V...

  3. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ... the purpose of the meeting: Begin meetings by stating “The purpose of this meeting is to _________." ...ambassador” from each affected team to be the representative in the meeting and then communicate outcomes...

  4. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...LbCpf1 RR variant 5' TYCV AsCpf1 RVR variant 5' TATV Campylobacter jejuni (CJ) 3' NNNNRYAC  Neisseria... as a scaffold linking the crRNA to Cas9 and facilitates processing of mature crRNAs from pre-crRNAs derived...

  5. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ... image, as well as a genetic method for the computational problem of edge detection. Tabor et al. subsequently...some promoter activity persisted in the inactive state. The dynamic range, defined as the difference between...

  6. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...Potential graduate students typically go through a rotation, which reveals their working habits, personality...the PI on any paper. Or perhaps, the lab has a reputation for not entering collaborations. Alternatively...

  7. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...SLIC compare to other cloning methods? SLIC’s limitations arise from its dependence on single-stranded ...may be assembled incorrectly. To overcome this limitation, one option is to perform a hierarchical assembly...

  8. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ... are positive for your marker would be a good statistic to compare between samples. In cases where there...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...

  9. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte. This signal...biosensor is the signal transducer which will quantitatively give a readout for whatever the bio-recognition...

  10. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...have a major impact on experimental design and interpretation of results. Take extra care when evaluating... present at both the injection site and in the putative projection neurons. When using this serotype to...

  11. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ... to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason you...a specific wavelength of light in response to excitation by a laser, which is then read by a detector ...

  12. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...BioBuilder curriculum is now taught in almost every state in the U.S. and in more than 30 countries around...The BioBuilder Learning Lab is a fully equipped, state-of-the-art lab embedded in LabCentral’s community...

  14. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...determine brightness are the extent to which the excitation light is absorbed and the efficiency by which...account, including the specs of your microscope (excitation wavelength, available emission filters, and detector...

  16. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion, for example...custom primers are especially helpful in verifying mutations that wouldn't be reached by sequencing from either...

  17. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...plasmid of all time, but was found to have a point mutation in the puromycin cassette that makes it less efficient...(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient...

  18. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...If you missed the webinar, you can watch the presentation and Q&A session on our YouTube channel. And ... a standard curve and somewhat more reliable quantitation. Differences in sample treatment across methods...

  20. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...excited to see how this exchange of genetic tools facilitates plant science. Challenge #3: Intellectual property...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree...
Showing: 461 - 480 of 719 results