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Addgene
Showing: 461 - 480 of 705 results

  1. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...plasmid of all time, but was found to have a point mutation in the puromycin cassette that makes it less efficient...(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient...

  2. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...If you missed the webinar, you can watch the presentation and Q&A session on our YouTube channel. And ... a standard curve and somewhat more reliable quantitation. Differences in sample treatment across methods...

  3. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ... to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason you...a specific wavelength of light in response to excitation by a laser, which is then read by a detector ...

  5. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...determine brightness are the extent to which the excitation light is absorbed and the efficiency by which...account, including the specs of your microscope (excitation wavelength, available emission filters, and detector...

  7. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte. This signal...biosensor is the signal transducer which will quantitatively give a readout for whatever the bio-recognition...

  8. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...have a major impact on experimental design and interpretation of results. Take extra care when evaluating... present at both the injection site and in the putative projection neurons. When using this serotype to...

  9. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...SLIC compare to other cloning methods? SLIC’s limitations arise from its dependence on single-stranded ...may be assembled incorrectly. To overcome this limitation, one option is to perform a hierarchical assembly...

  10. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ... are positive for your marker would be a good statistic to compare between samples. In cases where there...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...

  11. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...BioBuilder curriculum is now taught in almost every state in the U.S. and in more than 30 countries around...The BioBuilder Learning Lab is a fully equipped, state-of-the-art lab embedded in LabCentral’s community...

  13. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...excited to see how this exchange of genetic tools facilitates plant science. Challenge #3: Intellectual property...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree...

  14. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...Assign each meeting a leader from the group in a rotation. The leader for that meeting is responsible for...and meet to debrief on the topic (career) or presentation style (science). Have a potluck dinner. Invite...

  16. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...We are delighted at the early outcomes and the ecstatic reception by scientists. The success of these...candidates from internal referrals. Our long reaching reputation helps us hire great people from farther away ...

  17. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ... in the orientation of the two fragments. However, rigidity can be introduced through mutation, allowing...

  18. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...sometimes assumed that the direct method is more quantitative than the indirect method. But in reality, multiple...immunoimaging assays do not allow for absolute quantitative analysis of a sample. Instead, relative quantification...

  20. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...plasmid. Each gRNA combination can be tracked and quantitatively analyzed by sequencing its set of barcodes....: Forward oligo: 5’- CACCGNNNNNNNNNNNNNNNNNNNGTTTGGGTCTTCGAGAAGACCTATTCXXXXXXXXC -3’; Reverse oligo: 5...
Showing: 461 - 480 of 705 results