We narrowed to 691 results for: Tec
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TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m.... will not recognize a Fab fragment. In order to detect a Fab fragment, it either needs to be directly ...identified by a double-label immunofluorescence technique for monoclonal antibodies. J Histochem Cytochem...
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dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m....exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective small molecule...author on the recent paper describing the dTAG technology. Follow him on twitter @behnamnabet. References... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m.... the open windows in the middle of your talk. Technical difficulties turn your slide deck into a chalk...everyone! Everybody makes mistakes, encounters technical difficulties, and runs into unexpected stumbling... -
To Each HIS Own
TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m....Proteins with a Novel Metal Chelate Adsorbent. Bio/Technology. 1988; 6: 1321–1325. Janknecht R, de Martynoff...growth factor I from culture medium of E.coli. Bio/Technology. 1987, 5:379-382. Bornhorst JA and Falke JJ. ... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog PostPublishedDec. 8, 2022, 2:15 p.m.... Cas14 makes it a promising detection tool for rare, often hard to detect variants such as single nucleotide... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....laboratory situation or tissue collection method, so biotechnology companies have marketed several products that...These products, namely RNAlater® from Thermo and RNAProtect® from Qiagen, are ammonium sulfate-based solutions... -
Transferable Skills Guide: Cross-team Communication
TypeBlog PostPublishedNov. 21, 2019, 1:53 p.m....involved “plates,” and our scientists and lab technicians always knew from context what a “plate” was. ... the lab to be using words for their field or technology in a consistent way. Just creating a lexicon ... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....PubMed PMID: 29043675.Grefen C and MR Blatt. Biotechniques 2012. PubMed PMID: 23066669.Hecker A et al, ...Uppalapati and Woolley lab developed a phage display technique using small scaffold proteins to identify novel... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....Tools for studying bioelectricity Dyes and other technologies are now available for fluorescent reporting ...synthetic bioengineering." Current opinion in biotechnology 52 (2018): 134-144. PubMed PMID: 29684787. Oviedo... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....the steps you will have to take. Decide which technique you are going to adopt (i.e. restriction cloning...Find how-to videos for molecular biology and lab techniques ... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog PostPublishedSept. 22, 2015, 2:30 p.m....error rate of current NGS approaches allows for a detection limit of roughly 0.1%. Thus, rare, pre-existing...prior to treatment, there are small (previously undetectable) populations of cancer cells that are resistant... -
Plasmids for Endogenous Gene Tagging in Human Cells
TypeBlog PostPublishedApril 6, 2017, 1:02 p.m....control. But even with the advent of CRISPR/Cas9 technology, inserting large tags into a precise location...plasmid into the cells we use electroporation, a technique that briefly destabilizes the cell’s outer membrane... -
6 Tips for Grant Writing
TypeBlog PostPublishedOct. 18, 2016, 2:30 p.m....network of your peers. Furthermore, using new technologies to solve a problem can allow you to approach...different labs during your career; you learn new techniques and interact with new people. If you are looking... -
Adenoviral Vector Production and Troubleshooting
TypeBlog PostPublishedJune 14, 2018, 11:44 a.m....hexon proteins. The viral genome DNA is further protected by histone-like proteins forming the nucleocapsid... assays are commercially available for faster detection of RCA. 2nd Gen AdVs were designed to have increased... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....the Associate Director and Head of Gene Editing Technology at Beam Therapeutics, used a previously developed...activity and therapeutic application. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0491-6 Additional... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....Partnering with seqWell, we are using the plexWell technology to easily and quickly create Illumina sequencing...tags, fusion proteins, and selectable markers by detecting common features using Snapgene. If we find information... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....10.1021/acssynbio.9b00395 O’Brian, SP, et al., Biotechnol J. 2014. https://pubmed.ncbi.nlm.nih.gov/24390935...as time-lapse imaging or with high light power detection of molecules. Lee, J. et al., Science Advances... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....Yoshimi, K., & Mashimo, T. (2022). Genome editing technology and applications with the type I CRISPR system...for genome engineering in human cells. Nature Biotechnology, 37(12), 1471–1477. https://doi.org/10.1038/... -
Magnetic Control of Proteins: More than a Dream
TypeBlog PostPublishedSept. 24, 2024, 1:15 p.m....Lots of scientists work to invent novel techniques or to engineer improved tools for familiar applications...expressing good ol’ EGFP — showed a slight but detectable change in fluorescence when a small magnet was... -
Transferable Skills Guide: Managing a Team
TypeBlog PostPublishedJan. 30, 2018, 2:42 p.m....to avoid mistakes due to miscommunication and to detect trouble. I’m not comfortable with awkward silences...someone more experienced to guide you - is valuable. Protect your team Make you team feel safe. Your team members...