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We narrowed to 919 results for: addgene

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Showing: 461 - 480 of 919 results

  1. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...sequence. (A) Plasmid sequence from pLVUHshp53 (Addgene plasmid #11653). Full shRNA sequence is highlighted...boxes. (B) Plasmid sequence from pcDNA3-miR21 (Addgene plasmid #21114). The miRNA sequence is highlighted...button_text" is_json="true" %}{% raw %}"Browse Addgene's shRNA plasmid collection"{% endraw %}{% end_module_attribute...s12551-021-00902-w Additional resources on the Addgene blog RNA Interference in Plant Biology: New Tools...https://www.addgene.org/collections/mammalianrnai/","href_with_scheme":"https://www.addgene.org/collections... Plasmids 101 Posts Additional resources on addgene.org Mammalian shRNA Tools for RNAi Molecular Biology...

  2. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...vectors (for a more detailed explanation, check out Addgene's Lentiviral Guide). To produce lentiviral vectors...integrase-deficient packaging plasmid available at Addgene: psPAX2-D64V. This construct has the D64V mutation... how to package lentiviral vectors, check out Addgene’s Lentiviral Guide and our lentiviral production...Central PMCID: PMC9377332 More resources on the Addgene blog Viral Vectors 101: The Retroviral Life Cycle...every conversation! This blog post was written by Addgenie Rebecca Hutcheson.   References and resources... to All Things Lentivirus More resources on addgene.org Lentiviral Guide   Popular lentiviral vectors...

  3. Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)

    Type
    Blog Post
    Published
    July 26, 2018, 12:46 p.m.
    ...custom KTR design, plasmids are available from Addgene! References Kudo, T., Jeknić, S., Macklin, D.N...KTR plasmids here! Additional Resources on the Addgene Blog Learn about photoswitchable kinases Learn...protein phosphorylation Additional Resources on Addgene.org Find FRET plasmids Find fluorescent biosensors...

  4. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...PubMed Central PMCID: PMC3969860. Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using... Plasmids to Mammalian Cells Resources on the Addgene Website Visit our CRISPR Guide Pages Browse Our...

  5. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ...vectors to generate a kit which is now available at Addgene. “We called it cloning by mail,” Wood says, and...PMCID: PMC4353587. Additional Resources on the Addgene Blog Learn How the Clon Tracer Library Has Been...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled...

  6. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...should come as no surprise that those of us at Addgene believe in the power of sharing, and especially...And, last but not least (certainly not for us), Addgene launched this blog, A Better Way to Share Science...

  7. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ... which is similar to PR-T4P, is available from Addgene. If you use these published protocols and want ...genes, we encourage you to deposit them back with Addgene and expand the number of genes that other classrooms... PMCID: PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic...Models for learning about CRISPR Resources at Addgene.org Find plasmids from the Sánchez lab Find plasmids...

  8. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV...PubMed PMID: 25665714. Additional Resources on the Addgene Blog Check out our Viral Vectors Featured Topic... AAV in Addgene's Viral Service Check out our AAV Production Protocol Check out our AAV Purification Protocol...

  9. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...skill set Resources on the Addgene website Become of a member of the Addgene depositor community ...your scientific conference? Resources on the Addgene blog Read other posts on scientific sharing, scientific...

  10. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...in large sections of DNA can be more difficult. Addgene depositors have developed new CRISPR-based methods...please consider sharing them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, ... PMCID: PMC2782548 Additional Resources on the Addgene Blog Get the Basics of Homology Directed Repair... Used to Turn Genes On and Off Resources on Addgene.org Find Mammalian Expression Vectors Find Cre-Lox...

  11. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...prokaryotic terminators and deposited several with Addgene (1). Prokaryotic polyadenylation Although mostly...General overview BGH rbGlob SV40 Resources on the Addgene Blog Learn how the Promoter Controls Gene Expression...Field? Check out Our Plasmid Collections! Join the Addgene Community      ... all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference Pages Just Starting...

  12. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...published a protocol that would work for your system. Addgene has depositor submitted protocols and links to ...of CRISPR delivery. There are a few plasmids at Addgene that have been published as CRISPR testing tools...writing of this post. Additional Resources on the Addgene Blog Check out our CRISPR Featured Topic Page ...User (by a 1st Time CRISPR User) Resources on Addgene.org Browse CRISPR Plasmids for Mammalian Systems...

  13. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ... several hundred plasmids from this study with Addgene. Read on to learn more about their work, and how...PMCID: PMC4737758. Additional resources on the Addgene blog Learn about our cancer collection pages Read... Read all cancer blog posts Resources on Addgene.org Find plasmids for your research Find cancer research...

  14. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ... 93–103 Additional resources on the Addgene blog Browse Addgene's viral vectors articles Find tips and...working with viral vectors Resources on Addgene.org Check out Addgene's written and video protocols ...contamination periodically. For more tips, check out Addgene's video Getting Started with Tissue Culture below...environmental contaminants.  Figure 2: An Addgenie works in the tissue culture hood while wearing...

  15. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ... post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ready-to-use...next time you test an antibody (perhaps one from Addgene!), consider sharing your results so that others...References and Resources Additional resources on the Addgene blog:  Still trying to choose an antibody? Check...-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids here!  References...

  16. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for Immunocytochemistry...IHC validated or ICC validated antibodies from Addgene), or be prepared to run some extra controls and...three targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...10.1073/pnas.2018488117 Additional Resources on the Addgene blog Introduction to Antibodies Which Fluorescence...

  17. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...an instructional text file. What's available at Addgene? In total, we constructed and tested eight two-...in the table below and can be requested through Addgene. Their plasmid maps and GenBank files can be found...the plasmid maps and GenBank files uploaded to Addgene. We also recommend inserting self-cleaving hammerhead...PMCID: PMC4245323. Additional Resources on the Addgene Blog Learn How Piggy Bac Vectors Can Be Used for...of Our SynBio Posts Additional Resources on Addgene.org Visit Our SynBio Pages Check Out More Lu Lab...

  19. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ...targeting guide here! Additional resources on the Addgene blog Download the viral vectors 101 eBook Read...viral service Find retrograde AAVs at Addgene ...Learn more about retrograde AAVs Resources on Addgene.org Read our AAV guide Learn more about our viral...

  20. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...that expound on bench skills and experience. Most Addgene PhD level job descriptions clearly describe that....  You might also enjoy the other posts in the Addgene Blog Scientist Careers Series.    ...
Showing: 461 - 480 of 919 results