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Showing: 461 - 480 of 907 results

  1. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...vectors Learn about Addgene AAV Quality Control Process Resources on Addgene.org Find ready-to-use AAV...PubMed PMID: 25665714. Additional Resources on the Addgene Blog Check out our Viral Vectors Featured Topic... AAV in Addgene's Viral Service Check out our AAV Production Protocol Check out our AAV Purification Protocol...

  2. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ... which is similar to PR-T4P, is available from Addgene. If you use these published protocols and want ...genes, we encourage you to deposit them back with Addgene and expand the number of genes that other classrooms... PMCID: PMC304099. Additional Resources on the Addgene Blog Arabidopsis in Education Learning Synthetic...Models for learning about CRISPR Resources at Addgene.org Find plasmids from the Sánchez lab Find plasmids...

  3. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...skill set Resources on the Addgene website Become of a member of the Addgene depositor community ...your scientific conference? Resources on the Addgene blog Read other posts on scientific sharing, scientific...

  4. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...in large sections of DNA can be more difficult. Addgene depositors have developed new CRISPR-based methods...please consider sharing them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, ... PMCID: PMC2782548 Additional Resources on the Addgene Blog Get the Basics of Homology Directed Repair... Used to Turn Genes On and Off Resources on Addgene.org Find Mammalian Expression Vectors Find Cre-Lox...

  5. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...prokaryotic terminators and deposited several with Addgene (1). Prokaryotic polyadenylation Although mostly...General overview BGH rbGlob SV40 Resources on the Addgene Blog Learn how the Promoter Controls Gene Expression...Field? Check out Our Plasmid Collections! Join the Addgene Community      ... all of our Plasmids 101 Posts Resources on Addgene.org Browse our Plasmid Reference Pages Just Starting...

  6. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...published a protocol that would work for your system. Addgene has depositor submitted protocols and links to ...of CRISPR delivery. There are a few plasmids at Addgene that have been published as CRISPR testing tools...writing of this post. Additional Resources on the Addgene Blog Check out our CRISPR Featured Topic Page ...User (by a 1st Time CRISPR User) Resources on Addgene.org Browse CRISPR Plasmids for Mammalian Systems...

  7. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ... several hundred plasmids from this study with Addgene. Read on to learn more about their work, and how...PMCID: PMC4737758. Additional resources on the Addgene blog Learn about our cancer collection pages Read... Read all cancer blog posts Resources on Addgene.org Find plasmids for your research Find cancer research...

  8. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    Published
    Oct. 8, 2020, 1:15 p.m.
    ... 93–103 Additional resources on the Addgene blog Browse Addgene's viral vectors articles Find tips and...working with viral vectors Resources on Addgene.org Check out Addgene's written and video protocols ...contamination periodically. For more tips, check out Addgene's video Getting Started with Tissue Culture below...environmental contaminants.  Figure 2: An Addgenie works in the tissue culture hood while wearing...

  9. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for Immunocytochemistry...IHC validated or ICC validated antibodies from Addgene), or be prepared to run some extra controls and...three targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...10.1073/pnas.2018488117 Additional Resources on the Addgene blog Introduction to Antibodies Which Fluorescence...

  10. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ... post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ready-to-use...next time you test an antibody (perhaps one from Addgene!), consider sharing your results so that others...References and Resources Additional resources on the Addgene blog:  Still trying to choose an antibody? Check...-to-use antibodies from Addgene Designing a recombinant validation experiment? Find plasmids here!  References...

  11. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...an instructional text file. What's available at Addgene? In total, we constructed and tested eight two-...in the table below and can be requested through Addgene. Their plasmid maps and GenBank files can be found...the plasmid maps and GenBank files uploaded to Addgene. We also recommend inserting self-cleaving hammerhead...PMCID: PMC4245323. Additional Resources on the Addgene Blog Learn How Piggy Bac Vectors Can Be Used for...of Our SynBio Posts Additional Resources on Addgene.org Visit Our SynBio Pages Check Out More Lu Lab...

  12. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    Published
    Nov. 27, 2019, 2:03 p.m.
    ...targeting guide here! Additional resources on the Addgene blog Download the viral vectors 101 eBook Read...viral service Find retrograde AAVs at Addgene ...Learn more about retrograde AAVs Resources on Addgene.org Read our AAV guide Learn more about our viral...

  14. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...that expound on bench skills and experience. Most Addgene PhD level job descriptions clearly describe that....  You might also enjoy the other posts in the Addgene Blog Scientist Careers Series.    ...

  15. New Antibody Animation!

    Type
    Blog Post
    Published
    Feb. 22, 2024, 3:13 p.m.
    ...isotypes found in mammals.      Subscribe to Addgene's YouTube channel for more helpful animations, useful...

  16. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...interest; more recently we have also been using Addgene CRISPR vectors to generate cell lines with knockouts...figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning method procedure...som.umaryland.edu.   Additional Resources on the Addgene Blog Tips for a 1st Time CRISPR User (by a 1st...Plasmids 101: Mammalian Vectors Resources on Addgene.org Check Out Our Protocols Pages Browse Fluorescent...

  17. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...own mutant flies. Find Drosophila Resources at Addgene The birds and the bees when it comes to fruit ...basic knowledge to start working with flies. Like Addgene, the Drosophila community is highly sharing-oriented... a “pound symbol”. Additional Resources on the Addgene Blog Methods for Site Specific Recombination in...Drosophila Learn about Cre-Lox Resources on Addgene.org Find Drosophila Plamids Drosophila CRISPR Resources...

  18. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ...Antibodies Resources on Addgene.org Find recombinant antibodies at Addgene Find plasmids encoding recombinant...the-process-of-fixation-and-the-nature-of-fixatives/  Additional resources on the Addgene blog: Antibodies 101: Introduction to Immunofluorescence...recombinant antibodies at Addgene  ...

  20. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ... complete collection of protocol videos on the Addgene YouTube page. ...
Showing: 461 - 480 of 907 results