We narrowed to 526 results for: arl
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TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....the protocol, samples can be expanded to ~4-10x linearly! This expansion physically separates molecules...
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Great Results Start with Great Standard Curves
TypeBlog PostPublishedAug. 15, 2024, 1:15 p.m....consistent dilution factors, change pipette tips regularly, and mix samples very well. Use duplicates or... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m...., Zhan C, Malm M, Field R, Turner R, Sellick C, Varley P, Rockberg J, Chotteau V. Small-scale bioreactor... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....extraction method described in Kim and Morrison is nearly identical to that of commercially available kits... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m.... PCR set-up Setting up colony PCR reactions is nearly identical to preparing a standard PCR reaction:... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....References 1. Buchholz, Ursula J., Stefan Finke, and Karl-Klaus Conzelmann. "Generation of bovine respiratory... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m..... Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....particular area, e.g. education, poverty, environment, early childhood development, or community development?... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....able to work in different parts of the world in my early research career. I've found that research institutes... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m.... type of transcription reporting was among the earliest uses of GFP. Fluorescence resonance energy transfer... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....you’ll never know it so don’t miss out on those early kisses. The laundry or dishes can wait. Also, be... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....Central PMCID: PMC1299251. 3. Emilsson, Valur, and Charles G. Kurland. "Growth rate dependence of transfer... -
Neuronal labeling with Spaghetti Monster
TypeBlog PostPublishedAug. 14, 2018, 12:42 p.m....reliable primary antibodies for their detection, particularly when antibodies to the POI are non-specific,... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m.... other proteins. You can detect base editing as early as 48-72 hours post-transformation/transfection ... -
Transferable Skills Guide: Creativity
TypeBlog PostPublishedJan. 18, 2022, 2:15 p.m.... of encouraging dissent, the next tip may be particularly relevant to you. Elect a devil’s advocate. This... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog PostPublishedSept. 16, 2020, 1 p.m....they saw indel formation and phenotypic changes as early as 1 week post-injection. Livers from these mice... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog PostPublishedSept. 9, 2025, 1:15 p.m....systemically (retro-orbitally, RO; or intracerebroventricularly, ICV) or locally (stereotaxically, STX... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog PostPublishedJuly 8, 2025, 1:15 p.m....depends directly on the choices you make during the early stages of the process. Some questions to start with... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....machinery. Alternatively, some slow-growing cells, particularly mammalian cells like neurons, may not replicate...