We narrowed to 529 results for: arl
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TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....droplet separation (Witte et al., 2016). This is particularly important if your amplicon is longer than 150...
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MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....robustly, quickly, and simply. We hope that you can similarly apply MXS chaining to your experimental needs.... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m....transformant. Use young plants: If you transform early, your plants will still have plenty of time to produce... -
CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog PostPublishedSept. 6, 2018, 12:02 p.m....transfection (B in the figure above), are in the early stages of development. David Liu’s Lab has demonstrated... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m....difficult it is to trace individual cell’s axons, particularly the two green neurons near the arrows. When ... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m....journal articles. From paper to plasmid Authors regularly indicate that their plasmids are available from... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....adventure, just remember that even though these early steps don’t even involve antibodies, they are still... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....cargos must be flanked by the L and R ends - thus, scarless insertions are not currently possible, unlike ... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m.... PCR set-up Setting up colony PCR reactions is nearly identical to preparing a standard PCR reaction:... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog PostPublishedMarch 17, 2016, 2:30 p.m....References 1. Buchholz, Ursula J., Stefan Finke, and Karl-Klaus Conzelmann. "Generation of bovine respiratory... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m..... Sticky-end mediated gene transfer would be particularly helpful for targeting non-dividing cells, which... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....particular area, e.g. education, poverty, environment, early childhood development, or community development?... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....able to work in different parts of the world in my early research career. I've found that research institutes... -
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)
TypeBlog PostPublishedMay 15, 2014, 3:33 p.m.... type of transcription reporting was among the earliest uses of GFP. Fluorescence resonance energy transfer... -
Negotiating Work and Life: How to Find the Joy
TypeBlog PostPublishedMarch 20, 2014, 3:04 p.m....you’ll never know it so don’t miss out on those early kisses. The laundry or dishes can wait. Also, be... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog PostPublishedSept. 9, 2025, 1:15 p.m....systemically (retro-orbitally, RO; or intracerebroventricularly, ICV) or locally (stereotaxically, STX... -
Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)
TypeBlog PostPublishedJuly 8, 2025, 1:15 p.m....depends directly on the choices you make during the early stages of the process. Some questions to start with... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....machinery. Alternatively, some slow-growing cells, particularly mammalian cells like neurons, may not replicate... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome. Since replication... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....our more popular libraries, you might receive a similarly-sized aliquot from an amplification done by Addgene...