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Showing: 461 - 480 of 756 results

  1. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...therefore will require crosslinking, which uses formaldehyde or UV light to create strong but reversible ...actively interacting with, reducing background information. Your options for this step are sonication, which... with a PCR purification kit or with a phenol-chloroform extraction.    Fig. 1: Schematic overview...

  2. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ... genes, researchers at the Genetic Pertubation Platform at the Broad Institute have expanded the genetic...-based method for screens. Cas12a’s ability to perform multiplex editing within a single expression cassette...used to create a genome-wide library capable of performing similarly to Cas9 screens in a compacted library...

  3. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ... still in its infancy. A long timeline from transformation to testing and a lack of well-characterized...s made even more difficult by the fact that transformation methods have also been patented. In order to.... We will also be working with open source, transformation technologies like those pioneered by Ewen Mullins...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ... to support sharing of CRISPR constructs and information across the globe.  “Read the Addgene guide: https...barrier for depositors and requestors alike. We transformed this process by aligning institutions with standardized...the Neuroscience AntiBody Open Resource (NABOR) platform, supported by an NIH BRAIN Initiative. We also...

  5. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...methods where binding relies on a specific protein conformation, the polyhistidine-metal ion interaction is ...this one developed by Dr. James Trimmer’s lab, to perform assays that they otherwise would not be able to...polyhistidine tags. For more antibody-related information, sign up to receive alerts for antibody posts...

  6. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type
    Blog Post
    Published
    April 23, 2018, 2 p.m.
    ...control of gene expression, Aranda-Díaz et al transformed yeast with both a GAL4/estradiol controlled transcription...factor. They then induced cultures of these transformants with various concentrations of estradiol and...factors 1 and 2. See Aranda-Díaz et al. for more information. The El-Samad lab predicts that this level of...

  7. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...antibody. Perform a second series of wash steps. Activate the signaling molecule and perform the assay...sample with the primary (unconjugated) antibody. Perform a series of wash steps to remove any unbound or...

  8. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...immunogen. A different type of purification can be performed to isolate antibodies only recognizing your protein...steps keep the cost low compared to other antibody formats. This low barrier to entry makes polyclonal antibodies... detect a protein in low abundance or whose conformational state may change. If your protein might become...

  9. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...relevant host biomarkers. The lab validated their platform on clinical stool samples by comparison to qPCR...Gilliamella apicoli. The plasmids could also be transformed into Alphaproteobacteira, Betaproteobacteria,... al. "A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers...

  10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    Published
    March 19, 2019, 1:31 p.m.
    ...the LOV2 domain unfold, disrupting the protein conformation and thus, inhibiting function of Acr. This allows...photoswitchable proteins, which change in protein conformation in response to light, offers a way to spatially...of the photoswitchable cPYP, which changes in conformation in response to blue light (445nm). The discovery...

  11. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...as “sample,” “material,” “item,” and “format” all received formal definitions in our lexicon. We created...container, when applicable Any other relevant information Prescribing an official name to all of our plates...

  12. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...very interesting talk by Prof. Erin M Schuman, a former Caltech researcher, now Director at the Max Planck... from them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized...provides both quantitative and spatio-temporal information about the turnover of specific endogenous proteins...

  13. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...gene in frame with an MCS. E. coli that were transformed with the empty vectors expressed the ccdB gene...applications. While these two strains ultimately perform the same function, there is some evidence that ...that ccdB Survival™ can be more difficult to transform, depending upon the specific plasmids being used. ...

  14. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...background papers you could get your hands on, formulated a hypothesis, and planned out your key experiments...so look at epitope target, source, and other information to make sure you’re not trying to choose between...Antibody-based assays are complex and antibodies may perform differently in different samples and experimental...

  15. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...cloning strategy designed by synthetic biologists to perform large scale, one-tube cloning reactions. In this...used to assemble multigene constructs capable of performing production and subsequent necessary modification... them to these organisms via another optimized platform. Gene Stacking As the name implies, gene stacking...

  16. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...packaging systems were designed to prevent the formation of replication competent lentivirus (RCL). Replication...requires the viral transactivator, Tat. For more information on the different generations of lentiviral packaging...to obtain the best titer possible. While this information provides a great starting point, it may not translate...

  18. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...CeNGEN is planned for August 15th, 2019. For more information about the aims and approach of CeNGEN, check ... C. elegans. This database provides detailed information about C. elegans 3’UTRs structures, alternative...PMC3531189. Mangone, Marco, et al. "UTRome. org: a platform for 3′ UTR biology in C. elegans." Nucleic acids...

  19. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...the six months of my co-op, I have conducted informational interviews with nine Addgenies. There are a ...interested to learn more when I was introduced to bioinformatics in the previous programs. I thought, ‘Let me...flexibility and be up to date with the current world. Bioinformatics it is.’” Though she misses the bench, the change...

  20. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ... containing the active promoters at Addgene. Performance of synthetic promoter AAVs in mice All of these...promoter plasmids! Comparing synthetic promoters performance in mouse, monkey, and human retinas Next, the...in monkey and human retinas. By comparing the performance of promoters across these three species, the ...
Showing: 461 - 480 of 756 results