We narrowed to 514 results for: gan

1. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

   Type

   Blog Post

   Published

   Aug. 27, 2020, 1:15 p.m.

   ... such resources in less genetically tractable organisms lags behind. BiFC can also be used to screen for...

2. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   Published

   July 30, 2020, 1:15 p.m.

   ...funding. I’ve been aware of these problems since I began my own career over 20 years ago, and I’m sure they...

3. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

   Type

   Blog Post

   Published

   July 16, 2020, 1:15 p.m.

   ...of COVID-19 effect on plasmid requests Before we began any sophisticated analyses, we first visualized ...

4. Droplet Digital PCR for AAV Quantitation

   Type

   Blog Post

   Published

   Jan. 16, 2020, 2:18 p.m.

   ...Since Addgene began producing AAV vector preparations, we have used quantitative PCR to evaluate the ...

5. Plasmids 101: Choosing an Antibiotic Resistance Gene

   Type

   Blog Post

   Published

   Oct. 10, 2023, 1 p.m.

   ...Resistance Stability Time Saving?  Works for multiple organisms? Compatible with most bacteria? No satellite colonies...

6. Viral Vectors 101: The Retroviral Lifecycle

   Type

   Blog Post

   Published

   July 27, 2023, 1:15 p.m.

   ...Telesnitsky, A. (2008). Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus...

7. Tips for arabidopsis transformation

   Type

   Blog Post

   Published

   Oct. 25, 2018, 1:23 p.m.

   ...Stanford University. Arabidopsis is a fantastic model organism for many reasons, not the least of which is ease...

8. MXS Chaining

   Type

   Blog Post

   Published

   Feb. 7, 2017, 3:30 p.m.

   ...however, be appropriate for cloning genes from organisms with high CpG dinucleotide representation. The...

9. Tips from the Repository Trenches: Using Barcodes to Track Samples

   Type

   Blog Post

   Published

   Oct. 6, 2015, 2:30 p.m.

   ...just starting a lab, or are looking for a way to organize samples within your lab, we highly recommend using...

10. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    Published

    April 23, 2014, 1:08 p.m.

    ... Kendall Morgan...

11. Negotiating Work and Life: How to Find the Joy

    Type

    Blog Post

    Published

    March 20, 2014, 3:04 p.m.

    ...temple, my kids’ school, and with professional organizations supporting women in science and career development...

12. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...vitro and in vivo, including in human retinal organoids and live mice. Importantly, REVeRT avoids the ...

13. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...enrich adult mouse hematopoietic stem cells. Its ligand(s) has not been identified. While LY6A or other...

14. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    Published

    July 2, 2019, 12:51 p.m.

    ...applications require such optimized tools. This is elegantly demonstrated by the Qi Lab, which developed a ...

15. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...gene gets its name based on what happens to an organism when a gene/protein is depleted in a knockout ...

16. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...NGG VQR variant D1135V, R1335Q and T1337R NGAN or NGNG EQR variant D1135E, R1335Q and T1337R...

17. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...drosophila, worms and mammals (Browse plasmids by model organism, here).  More information on the various delivery...

18. Bioinformatics at Addgene

    Type

    Blog Post

    Published

    July 14, 2022, 1:15 p.m.

    ... collection of scripts evolved into a single, organized pipeline and accelerated the processing of our...

19. Viral Vectors 101: Pseudotyping

    Type

    Blog Post

    Published

    April 20, 2021, 2:30 p.m.

    ...more safely. Recently, many SARS-CoV-2 researchers began pseudotyping the SARS-CoV-2 spike protein into a...

20. CRISPR-based Genome Editing Systems For Editing in E. coli

    Type

    Blog Post

    Published

    April 13, 2021, 1:15 p.m.

    ...on the escape mechanism of genome editing in microorganisms which could be applied to optimization of genome...