We narrowed to 794 results for: mpl

...in the biological sciences and techniques that simplify and expedite research. We recognize, however, ...Blog is a great way to get your name out there! Completed blog posts are also great to include on CVs and... blog! To sign up to write for the Addgene blog simply email us. We can’t guarantee that all those who...

...be used to cut the DNA sequence. With a repair template, CRISPR can be used to introduce a functional ...single base changes in the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases...CRISPR nucleases that can’t cut DNA (dCas9, for example) that are used to increase or decrease expression...

.... To start the AAV packaged on request process, simply navigate to your plasmid of interest. If it’s eligible...received, we’ll review for technical and legal compliance, and then send you an email with a link to order...There are no quotes or special processes involved; simply request your plasmid of interest in your desired...

...antibodies, for example through the use of less toxic adjuvants; 2) generating complex antibody chips ... assessment of many protein targets in a single sample; 3) producing recombinant, partially humanized ...

...cultural shifts that can have bigger impacts. For example, if you joined an office where everyone used a ...long-term goals to stay motivated and to feel accomplished. In addition to doing some of the quick things... about this, and even has a list of biological samples that are stored long term at -70 °C. My Green Lab...

...Addgene, we try to make the process as smooth and simple as possible. We are the Customer Support Specialists... Placing an order on Addgene’s website is quite simple, even if you’re using a PO number. Follow these...time, your organization will have net 30 days to complete payment for your order.  Still have questions?...

...visualization in vivo using ultrasound (for an example see the figure below). In addition to low costs...Approaches to Presenting the Microbial World’ showcased complex connections and ideas in various visualized approaches...gifs created by the Amoeba Sisters. Here’s one example of how they “bridge the jargon gap using gifs”:...

...needs of employees, and the research environment.   What do you hope Addgene will accomplish in the near...creates a compensation philosophy that helps guide employee compensation and also serves as an objective body...

...technique and be able to talk and own these accomplishments. Employers and academic departments want to be able...finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You have learned...

...heart and soul of Addgene’s mission), but legal complexities can arise when scientists are sharing their ...facilitating the exchange of not just the physical samples, but also the legal documentation that allows you...Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!) and we’ll take...

... of 17 signaling pathways that are frequently implicated in cancer cell proliferation, survival, differentiation...they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when...ability to survive in certain environments, for example. “We’ve been contacted by investigators all over...

...Thousands of mutations have been implicated in cancer. Yet, the majority of them remain uncharacterized... of mutations they identified included gene amplifications and fusions, missense mutations, and indels...scientists understand how a given gene is involved in a complex pathway. Some of the most heavily-studied genes...

...should receive the clinical sample to test their phages. Because the sample is limited, only the labs that...targeting organisms closely related to the clinical sample. For treatment, it is best to identify a cocktail...

...the guesswork out of protein purification through simple and efficient multiplexing. pCri plasmids are divided...one need to remove the tag post-purification. A sample of pCri constructs: pCri-1a, -4a and -9a. 6xHis...plasmids are available either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros...

...academic and industry scientists. This partnership complemented each other's strengths: Ginkgo Bioworks’ infrastructure...distribute such a large deposit. Once the project is complete, Ginkgo will have synthesized approximately 3,... their plasmids available beyond academia. For example: Plasmids from the Michael J. Fox Foundation are...

... a fast endophyte screening system and plans to employ the light‐inducible bacterial expression system...alternative source of essential omega-3 fatty acids complementing vegetable oil, a somewhat poor natural source...them. By using yeast integration vectors (see for example this yeast cloning kit) she intends to make yeast...

...given ion - we’ll use calcium biosensors as an example. Many calcium biosensors include GFP fused to calmodulin...reticulum (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted calcium...available, and the conditions of your experiment. For example, does the fluorescent tag need to be pH stable ...

...sequence used as an initiation point for PCR amplification or sequencing. Primers can be exploited for ...clone into. Regardless, once the cloning steps are complete, the vector containing the newly inserted gene...and troubleshooting tips to make plasmid usage as simple and straightforward as possible. If you have a ...

... or condition of interest using a simple keyword search. For example, searching the term "breast cancer...broadest possible scope of screens to be curated, for example phenotype screens with read-outs other than cell...

...and cell lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations. The...change offers a significant improvement and simplification of the original technique, specifically in ...CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids for isolating...