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We narrowed to 789 results for: mpl

Showing: 461 - 480 of 789 results

  1. Bringing Sustainable Practices to the Lab: Easy Action Items

    Type
    Blog Post
    Published
    April 22, 2019, 12:26 p.m.
    ...cultural shifts that can have bigger impacts. For example, if you joined an office where everyone used a ...long-term goals to stay motivated and to feel accomplished. In addition to doing some of the quick things... about this, and even has a list of biological samples that are stored long term at -70 °C. My Green Lab...

  2. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...Addgene, we try to make the process as smooth and simple as possible. We are the Customer Support Specialists... Placing an order on Addgene’s website is quite simple, even if you’re using a PO number. Follow these...time, your organization will have net 30 days to complete payment for your order.  Still have questions?...

  4. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    Published
    Aug. 28, 2018, 12:04 p.m.
    ...visualization in vivo using ultrasound (for an example see the figure below). In addition to low costs...Approaches to Presenting the Microbial World’ showcased complex connections and ideas in various visualized approaches...gifs created by the Amoeba Sisters. Here’s one example of how they “bridge the jargon gap using gifs”:...

  5. Developing Transferable Skills During Science Training

    Type
    Blog Post
    Published
    June 3, 2014, 2:14 p.m.
    ...technique and be able to talk and own these accomplishments. Employers and academic departments want to be able...finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You have learned...

  6. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ...heart and soul of Addgene’s mission), but legal complexities can arise when scientists are sharing their ...facilitating the exchange of not just the physical samples, but also the legal documentation that allows you...Addgene helps: For Providers: One-time Deposit: Simply deposit with Addgene (it’s FREE!) and we’ll take...

  7. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    Published
    March 22, 2016, 2:30 p.m.
    ... of 17 signaling pathways that are frequently implicated in cancer cell proliferation, survival, differentiation...they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when...ability to survive in certain environments, for example. “We’ve been contacted by investigators all over...

  8. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...Thousands of mutations have been implicated in cancer. Yet, the majority of them remain uncharacterized... of mutations they identified included gene amplifications and fusions, missense mutations, and indels...scientists understand how a given gene is involved in a complex pathway. Some of the most heavily-studied genes...

  10. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ... are not generated from a plasmid. If you, for example, prefer to deliver your CRISPR components as RNPs...and you can search by any part of the data. For example, entering ‘C. elegans’, or ‘elegans’ will filter... a single space to get even more specific. For example, to show the gRNAs that were used for activation...

  11. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...the guesswork out of protein purification through simple and efficient multiplexing. pCri plasmids are divided...one need to remove the tag post-purification. A sample of pCri constructs: pCri-1a, -4a and -9a. 6xHis...plasmids are available either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros...

  12. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    Published
    June 23, 2020, 1:15 p.m.
    ...academic and industry scientists. This partnership complemented each other's strengths: Ginkgo Bioworks’ infrastructure...distribute such a large deposit. Once the project is complete, Ginkgo will have synthesized approximately 3,... their plasmids available beyond academia. For example: Plasmids from the Michael J. Fox Foundation are...

  13. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    Published
    April 2, 2020, 6:17 p.m.
    ...sequence used as an initiation point for PCR amplification or sequencing. Primers can be exploited for ...clone into. Regardless, once the cloning steps are complete, the vector containing the newly inserted gene...and troubleshooting tips to make plasmid usage as simple and straightforward as possible. If you have a ...

  15. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...and cell lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations. The...change offers a significant improvement and simplification of the original technique, specifically in ...CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids for isolating...

  16. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...Pinterest for scientists”? ReFigure extension is a simple Chrome plugin that allows you to save any image... even include your own data in a ReFigure. For example, you might include a confirmatory image to show...knowledge of which findings are reproducible. For an example, see our ReFigures on the therapeutic use of anti-CD47...

  17. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ...this frequency can indicate behavioral state, for example, if someone is awake, agitated, or asleep. Though...performed in a live awake animal by surgical implantation of the probe into a specific brain region. While...neurotransmitters in various species and while exhibiting complex behaviors. Find dopamine and other biosensors in...

  19. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...given ion - we’ll use calcium biosensors as an example. Many calcium biosensors include GFP fused to calmodulin...reticulum (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted calcium...available, and the conditions of your experiment. For example, does the fluorescent tag need to be pH stable ...

  20. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ... of origins of replication out there so, for simplicity’s sake, we've ignored those used in eukaryotic...manipulated with mutations in the replicon. For example, the pMB1 ori maintains about 20 copies per cell...sensitive; others ori's can be "tricked" into amplifying more copies with the addition of chloramphenicol...
Showing: 461 - 480 of 789 results