... of 17 signaling pathways that are frequently implicated in cancer cell proliferation, survival, differentiation...they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when...ability to survive in certain environments, for example. “We’ve been contacted by investigators all over...

...should receive the clinical sample to test their phages. Because the sample is limited, only the labs that...targeting organisms closely related to the clinical sample. For treatment, it is best to identify a cocktail...

...Thousands of mutations have been implicated in cancer. Yet, the majority of them remain uncharacterized... of mutations they identified included gene amplifications and fusions, missense mutations, and indels...scientists understand how a given gene is involved in a complex pathway. Some of the most heavily-studied genes...

... are not generated from a plasmid. If you, for example, prefer to deliver your CRISPR components as RNPs...and you can search by any part of the data. For example, entering ‘C. elegans’, or ‘elegans’ will filter... a single space to get even more specific. For example, to show the gRNAs that were used for activation...

...the guesswork out of protein purification through simple and efficient multiplexing. pCri plasmids are divided...one need to remove the tag post-purification. A sample of pCri constructs: pCri-1a, -4a and -9a. 6xHis...plasmids are available either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros...

...academic and industry scientists. This partnership complemented each other's strengths: Ginkgo Bioworks’ infrastructure...distribute such a large deposit. Once the project is complete, Ginkgo will have synthesized approximately 3,... their plasmids available beyond academia. For example: Plasmids from the Michael J. Fox Foundation are...

...and cell lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations. The...change offers a significant improvement and simplification of the original technique, specifically in ...CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids for isolating...

...Pinterest for scientists”? ReFigure extension is a simple Chrome plugin that allows you to save any image... even include your own data in a ReFigure. For example, you might include a confirmatory image to show...knowledge of which findings are reproducible. For an example, see our ReFigures on the therapeutic use of anti-CD47...

...sequence used as an initiation point for PCR amplification or sequencing. Primers can be exploited for ...clone into. Regardless, once the cloning steps are complete, the vector containing the newly inserted gene...and troubleshooting tips to make plasmid usage as simple and straightforward as possible. If you have a ...

... or condition of interest using a simple keyword search. For example, searching the term "breast cancer...broadest possible scope of screens to be curated, for example phenotype screens with read-outs other than cell...

... a fast endophyte screening system and plans to employ the light‐inducible bacterial expression system...alternative source of essential omega-3 fatty acids complementing vegetable oil, a somewhat poor natural source...them. By using yeast integration vectors (see for example this yeast cloning kit) she intends to make yeast...

...given ion - we’ll use calcium biosensors as an example. Many calcium biosensors include GFP fused to calmodulin...reticulum (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted calcium...available, and the conditions of your experiment. For example, does the fluorescent tag need to be pH stable ...

...this frequency can indicate behavioral state, for example, if someone is awake, agitated, or asleep. Though...performed in a live awake animal by surgical implantation of the probe into a specific brain region. While...neurotransmitters in various species and while exhibiting complex behaviors. Find dopamine and other biosensors in...

...expressed. Once the design and construction steps are complete, it’s time to make virus! The constructed viral...the biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated... to achieve different biological outcomes. For example, some viruses integrate into the host genome (lentivirus...

... of origins of replication out there so, for simplicity’s sake, we've ignored those used in eukaryotic...manipulated with mutations in the replicon. For example, the pMB1 ori maintains about 20 copies per cell...sensitive; others ori's can be "tricked" into amplifying more copies with the addition of chloramphenicol...

...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ... like as long as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas...also drive different levels of expression. For example, CAG is a strong promoter that drives very high...

...in Columbus, Ohio, ABRC ships more than 100,000 samples to researchers and educators in 60 countries each...Engineering the Plant Genome Using CRISPR/Cas9 pSiM24: Simplifying Plant Genetic Engineering Resources on Addgene.org...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants Parts...

...permanently attached to a protein, making them hard to amplify and image for long periods of time.  Are antibody... scientists fused them to sequences called complementarity determining regions (CDRs, or loops) of an ...demonstrated that the HA frankenbody can be used to amplify fluorescent signals in in vivo imaging: the 10xHA...

...phenotype typically emerges. Every three days, a sample of cells was collected for scRNA-seq analysis and...tended to have similar reprogramming outcomes. For example, cells with shared CellTagD13 tags often had similar...et al. "CellTag Indexing: genetic barcode-based sample multiplexing for single-cell genomics." bioRxiv...

...jGCaMP7 sensors with different properties (for example, overall brightness or on/off kinetics) that makes...electrical activity in the brain was measured with implanted electrodes. The Archon1 voltage reporter allows...quantify targeting of particular cell types. For example, the mDlx enhancer restricts expression to GABAergic...