We narrowed to 526 results for: proc

1. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

   Type

   Blog Post

   Published

   June 12, 2014, 3:30 p.m.

   ...no PCR, no gel purification; the whole cloning process is so simplistic once you have your parts. That...

2. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   Published

   Sept. 6, 2018, 12:02 p.m.

   ... to genome editing of mouse zygotes, through a process known as CRISPR-EZ (CRISPR RNP Electroporation ...

3. Plasmids 101: Secondary Nanobody Toolbox

   Type

   Blog Post

   Published

   Feb. 27, 2018, 2:04 p.m.

   ...exclusively recognize only one primary antibody. This process can be avoided direct antibody fluorophore conjugates...

4. Transferable Skills Guide: Identifying Your Transferable Skills

   Type

   Blog Post

   Published

   Jan. 11, 2018, 3:29 p.m.

   ...and train everyone on your team about that new process. You stepped up and got your team on the same page...

5. CRISPR 101: RNA Editing with Cas13

   Type

   Blog Post

   Published

   July 31, 2020, 12:30 p.m.

   ...RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270...

6. Capturing a Moment with Marc Zimmer’s “The State of Science”

   Type

   Blog Post

   Published

   July 30, 2020, 1:15 p.m.

   ...consequence of the undermining of the scientific process and the marginalization of science, particularly...

7. Isolating Genomic Regions of Interest with the CRISPR System

   Type

   Blog Post

   Published

   May 30, 2019, 12:44 p.m.

   ... of a specific genomic region was an intensive process. One method required insertion of a recognition...

8. Uncovering Ancestral Bias in CRISPR Research

   Type

   Blog Post

   Published

   Nov. 29, 2022, 2:15 p.m.

   ...with an ancestry-based bias.   The correction process does not stop there. Currently, they are working...

9. Negative Can Be Positive: Open AAV Data with Addgene

   Type

   Blog Post

   Published

   Sept. 6, 2022, 1:15 p.m.

   ...expression can take months (or longer), yet the process is often not detailed in the primary literature...

10. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type

    Blog Post

    Published

    July 1, 2025, 1:15 p.m.

    ... the sample itself — so easy that this simple procedure is often taken for granted. In this post, we take...

11. Getting to Know Expansion Microscopy

    Type

    Blog Post

    Published

    June 20, 2023, 1:15 p.m.

    ....)  Step 5: Image   Figure 1: The ExM process allows you to physically magnify a sample so you...

12. Plasmids 101: Colony PCR

    Type

    Blog Post

    Published

    May 12, 2016, 2:30 p.m.

    ...sequencing to verify the insert sequence before proceeding with your experiment. Shorter amplicons tend...

13. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type

    Blog Post

    Published

    March 17, 2016, 2:30 p.m.

    ...systems allow researchers to investigate viral processes in extreme detail. The mechanism of a particular...

14. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type

    Blog Post

    Published

    May 15, 2014, 3:33 p.m.

    ... enabling the study of dynamic, physiological processes. Re-engineering GFP to increase its range of color...

15. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type

    Blog Post

    Published

    Sept. 13, 2018, 1:55 p.m.

    ...for identifying genes involved in a biological process. These screens provide an alternative to genome-wide...

16. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type

    Blog Post

    Published

    Feb. 18, 2020, 2:15 p.m.

    ...Culturing a scientific network We refer to the process of mentoring within a bin as Horizontal Transfer...

17. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    Published

    Sept. 17, 2019, 12:40 p.m.

    ...tracking software. Locomotion is a highly complex process on the level of individual neurons and in terms...

18. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ...depending on the context. Contraction is critical for processes such as cell movement/motility, development, cytokinesis...

19. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ... a guide RNA. The enzyme has dual activities: processing pre-CRISPR RNA into mature CRISPR RNA and cleaving...

20. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type

    Blog Post

    Published

    July 29, 2025, 1:15 p.m.

    ...kanamycin resistance gene for bacterial selection. The process for cloning the 20-nt guide RNA is identical for...