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Addgene

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We narrowed to 711 results for: tat

Showing: 461 - 480 of 711 results

  1. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ... image, as well as a genetic method for the computational problem of edge detection. Tabor et al. subsequently...some promoter activity persisted in the inactive state. The dynamic range, defined as the difference between...

  2. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...slower than fluorescent-based methods, such as excitation of Green Fluorescent Protein (GFP) because of...enhancers and untranslated regions, or the effect of mutations of these regulatory elements on gene expression...

  3. Finding and Joining Your Dream Lab

    Type
    Blog Post
    Published
    April 8, 2014, 2:50 p.m.
    ...Potential graduate students typically go through a rotation, which reveals their working habits, personality...the PI on any paper. Or perhaps, the lab has a reputation for not entering collaborations. Alternatively...

  5. 15 Years of Addgene: The Top 15 Plasmids

    Type
    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...plasmid of all time, but was found to have a point mutation in the puromycin cassette that makes it less efficient...(BB)-2A-Puro (PX459) V2.0. It contains a point mutation in the puromycin cassette that makes it less efficient...

  6. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    Published
    Sept. 15, 2020, 1:15 p.m.
    ...non-functional đť›˝-galactosidase. Complementing this mutation by expressing this deleted portion, for example...custom primers are especially helpful in verifying mutations that wouldn't be reached by sequencing from either...

  8. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    Published
    June 1, 2017, 2:30 p.m.
    ...determine brightness are the extent to which the excitation light is absorbed and the efficiency by which...account, including the specs of your microscope (excitation wavelength, available emission filters, and detector...

  9. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...SLIC compare to other cloning methods? SLIC’s limitations arise from its dependence on single-stranded ...may be assembled incorrectly. To overcome this limitation, one option is to perform a hierarchical assembly...

  10. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ... are positive for your marker would be a good statistic to compare between samples. In cases where there...characterize your samples may allow for better interpretation. Priyamvada Prathima has a research background...

  11. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...If you missed the webinar, you can watch the presentation and Q&A session on our YouTube channel. And ... a standard curve and somewhat more reliable quantitation. Differences in sample treatment across methods...

  12. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ... to use, not sensitive to light in its inactive state, and cheap. Unless there’s a specific reason you...a specific wavelength of light in response to excitation by a laser, which is then read by a detector ...

  13. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
    Published
    April 27, 2023, 1:15 p.m.
    ...have a major impact on experimental design and interpretation of results. Take extra care when evaluating... present at both the injection site and in the putative projection neurons. When using this serotype to...

  14. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...DNA, etc. The receptors job is to generate a quantitative signal upon recognizing the analyte. This signal...biosensor is the signal transducer which will quantitatively give a readout for whatever the bio-recognition...

  15. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...BioBuilder curriculum is now taught in almost every state in the U.S. and in more than 30 countries around...The BioBuilder Learning Lab is a fully equipped, state-of-the-art lab embedded in LabCentral’s community...

  17. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    Published
    July 22, 2014, 5:46 p.m.
    ...excited to see how this exchange of genetic tools facilitates plant science. Challenge #3: Intellectual property...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree...

  18. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...Assign each meeting a leader from the group in a rotation. The leader for that meeting is responsible for...and meet to debrief on the topic (career) or presentation style (science). Have a potluck dinner. Invite...

  19. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...selective small molecule binders. To overcome this limitation, we created a generalizable tag-based strategy...context. Immunoblotting is recommended, while quantitative proteomics experiments will provide the best...

  20. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...RANbody platform from the Sanes Lab overcomes this limitation and allows for the flexible design and small ...you own DNA ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy...
Showing: 461 - 480 of 711 results