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We narrowed to 707 results for: ust

Showing: 461 - 480 of 707 results
  1. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...special: Strong expression: A CMV promoter drives robust gene expression in mammalian cells. Flexible tagging... Antibody Data Hub. Perhaps these antibodies are just the tools you need to push the field forward! Browse...
  2. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...it off, allowing the cell to be excited over a sustained period of time (Gong et al., 2020). Light-gated...Bendifallah, I., Zhou, F., Zeitzschel, N., Ahlbeck, J., Augustin, S., Sauter, K., Papagiakoumou, E., Gottschalk...
  3. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...downright infuriating at worst. While it is tempting to just dive right in and start injecting your virus, a ...through the endogenous GFAP promoter results in robust transduction in the rat spinal cord following injury...
  4. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...genetic tools that consist of two parts, both of which must be present for a gene of interest to be expressed...Liqun Luo’s lab developed the Q system from a gene cluster in the fungus Neurospora crassa. The QUAS promoter...
  5. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...study. These are highly useful for the biotech industry, which is in the process of capitalizing on phage...easily varied by the cell and whose structures are just beginning to be understood. Therefore, in addition...
  6. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...few gRNAs to choose from that sequence preferences must largely be ignored. Created with BioRender.com... For C>T and A>G base editors, the intended edit must be in a 5 - 10 nt window relative to the PAM, and...
  7. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...tools but their use is limited in vivo because they must be excited by external light sources. These external... subcellular structures and gene expression. You must see the movies demonstrating Ca2+ dynamics in cardiomyocytes...
  8. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...worked the first time probably because I used a robust cell line. If your cells are sensitive, it might...efficient at editing” to introduce the gRNA, but I just randomly chose one of my stable pools. Viruses containing...
  9. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ... do so at a range of wavelengths, rather than at just one defined wavelength. Likewise, the emitted photons... spectrum shows two maxima, one local maximum at just under 500 nm and a global maximum at around 560 ...
  10. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ... the sample are constantly exposed to light, not just those in the focal volume. This leads to more rapid.... Springer (2006). 7. Yang, Weijian, and Rafael Yuste. In vivo imaging of neural activity. Nature Methods...
  11. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...CBEs and ABEs are base transition editors. They adjust the side groups of DNA bases and leave the core...Protospacer-Adjacent Motif A short sequence that must be present in the non-target strand of DNA for Cas9...
  12. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...with clinical endpoints. In the first scenario, robust and efficient generation of iPSCs is the number...Central PMCID: 5072705. 2. Sommer, Cesar A., and Gustavo Mostoslavsky. "The evolving field of induced pluripotency...
  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...blood brain barrier by endothelial transcytosis (Foust et al., 2009; Merkel et al., 2017), and these two...PMID: 19578477. PubMed Central PMCID: PMC2600441. Foust, K.D., Nurre, E., Montgomery, C.L., Hernandez, A...
  14. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Experiences (CUREs)Author: Jason PellettieriNew Acoustic Reporter Genes: Ultrasound Imaging of Gene ExpressionAuthors...
  15. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ...Yit Heng Chooi Lab at the University of Western Australia.    🤩 our lab in Bayliss Building @SMS_UWA is...
  16. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...annoying. To make it easier, try this quick trick. Just flip, and press! Lab tip #2: Tube flick trick...
Showing: 461 - 480 of 707 results