We narrowed to 904 results for: CAN

...which can be done through the align tab of our sequence analysis web-tool. Alternatively, you can use DNA-editing...available and provide some advice on the steps you can take to verify your own plasmids. Staying organized...This will ensure that future members of your lab can access and use the materials they need, and avoid...we have a standard backbone with one insert, and can use common primers to sequence from the backbone ... primers to sequence the important features. You can use primer design software such as Primer3 to help...first 50-100 base pairs of your sequencing read. You can expect to recover around 750 bp of clean sequence...analysis of a large number of sequencing reactions, you can make your life much easier by selecting the right...

...timely manner (within a few days or a week) so they can easily recall the encounter. As a side note, my guess...for awhile or ever so your LinkedIn profile link can be your new card. Follow your contact’s company on... about their new publications via Pubmed, so you can congratulate them when there’s good news.  Send an...an email if you have something to ask or say. It can be as simple as “it was nice meeting you.” Include...non-work topics and interests. Offer help when you can, instead of just asking for favors.  Don’t focus ...’s a waste of our time.  Networking connections can last years This is a graphic snapshot of my LinkedIn... network give rise to a starfish shape. You can ask me about the different arms of this starfish ...

...secondary antibodies can be used with different fluorescent labels and both targets can be successfully visualized...region may be changed.   Chimeric antibodies can help A chimeric antibody is made by changing an antibody... 2: Two primary antibodies with the same isotype can not be used for multiplexed indirect staining methods... visualized (bottom panel).    Can any antibody be converted? Isotype conversion is a unique characteristic...sequences are known and encoded on a plasmid, they can be easily manipulated into more useful tools. In ...the binding specificity to the original target and can be used with secondary antibodies against the new... some sequence from their parental species which can be bound by broadly reactive secondary antibodies...

...piece of DNA so that it can be easily cloned into a plasmid of interest. You can use similar processes ...reverse-complement of this sequence so that we can successfully amplify the ORF. You can generate the reverse-complement...It is helpful to choose restriction enzymes that can both function in the same buffer, as this will save...restriction site to improve cutting efficiency. You can generally add any 6 bases, but you should ensure ... reverse complement to get PCR amplification. We can start similarly, taking the final 18 bases of the...there is very little chance for mis-priming, so you can use a pretty wide range of annealing temperatures...

...Named for the "stellar explosion SUperNova," SunTag can help you turn up the brightness in your fluorescent...protein is to fuse it to a fluorescent protein. You can then study where the protein is localized, and how...Having seen that mitochondrial tracker GFP-mitoNEET can impair mitochondrial function, they examined the ... recommended for most imaging applications. What can you do with SunTag? Tanenbaum et al. performed a ... of different experiments in their paper, and so can you! In almost any case you would use a traditional...kDa for GFP alone. Although traditional FP fusions can also affect protein activity, half-life, or localization...many areas of research. Beyond fluorescence, SunTag can also be used to improve CRISPR-based activation of...

...that is fused to a nicking Cas9. This base editor can be used for A•T to G•C edits in protoplasts or plants...regulated not only temporally with doxycycline, but it can also be regulated spatially with Cre-recombination...backbones, and gRNA structure, numbers, and promoters can all be chosen. Read the paper in PLoS One Find...Mobile-CRISPRi” plasmids, a set of modular CRISPRi tools that can be used in diverse bacteria from various environments...broad inhibitory effects on Cas12a orthologs and can block Cas12a-mediated genome editing in human cells...markers. It has the advantage that CRISPR efficiency can be controlled by manipulating complementarity between...

...insert, liquid media can be faster and easier to work with. In either case, it can be useful to run a restriction...downstream applications. As individual libraries can vary significantly, it’s important that we have an amplification...because repeated amplifications of pooled libraries can lead to issues such as recombination and loss of ... for smaller libraries, chemical transformations can also be used. Efficiency for chemical transformations...your lab’s electroporator uses, etc. The DNA amount can be anywhere from 10 ng to 1 μg of DNA. It often correlates...individual electroporations. The specifics of this can be based on the manufacturer’s protocol for transformation...(on plates). In general, amplification on plates can help to ensure that diversity of the library is maintained...

...is constantly evolving. Variants of Cas proteins can be used for genome editing, activating gene expression... and well studied CRISPR system, N. meningitidis can also be used for genome editing. In this study, the...control samples to 0-10% in Acr samples. Acr proteins can also be used to prevent dCas9 tethering to DNA. Since...specific to a particular Cas protein while others can inhibit CRISPR enzymes from multiple species of bacteria...organized by the type of CRISPR-Cas system they block can be found here. How do anti-CRISPR proteins work?...). Figure 1: Anti-CRISPR proteins (Acr's) can block CRISPR activity in many ways such as inhibiting...Reduce off-target effects Prolonged Cas activity can increase the chance of off-target editing particularly...

...research can be a daunting challenge. With over 80,000 deposited plasmids from around the world, it can be ...Vector Type” you can filter by experimental purpose (e.g. CRISPR) Beyond search What can you do if you ... through to a different part of the website, you can now use the pulldown menu on the search page to quickly...screenshots show the range of different terms we can use to find materials from this publication. Searching...Searching for the title of a paper works You can also find the associated plasmids through the article’...primary investigator where the plasmid was created can narrow your search results to exactly what you need...Availability to Industry: Restrict search to plasmids which can be distributed to industry research scientists Viral...

... inexpensive and can be 5’-phosphorylated in house using a simple PNK reaction or can be purchased 5’-...required for its optimization and development before it can truly be pitted against CRISPR head-to-head.  Exciting...rather than an RNA guide as with Cas9. Third, it can be used to target “difficult” GC-rich regions of ...protein (probably while it is being synthesized) can form a complex with an ssDNA guide. Therefore, researchers...versatility of its delivery methods. Researchers can conveniently transfect/inject cells with in vitro...achieved by a few respondents suggesting that NgAgo can indeed be optimized for genome editing in mammalian...time is required for NgAgo optimization before we can make any major conclusions: That said, many people...