...targeting EvolvR to five dispersed regions of the target gene of spectinomycin, rpsE. These results not only uncovered...directed mutagenesis to generate specific mutations in your plasmids Use REPLACER Mutagenesis to mutate...and forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants....Berkeley, provides a new approach for generating novel genetic variants in bacteria. Read on to learn about...EvolvR’s on-target mutagenesis rate was 2.5 x 10-6 mutations per nucleotide per generation vs. 10 x 10-10 ...additional mutations to PolI3M generated PolI5M which increased EvolvR’s mutagenesis rate to ~10-3 mutations ...than one genomic site for mutagenesis, a feature which they used to generate bacteria resistant to both...

...means summer is right around the corner. Here at Addgene, we’re always inspired by the new seasons, so we've...complex questions, and giving tips for consistently generating solid data. If you’re ready for the next steps...We’re very excited to be launching the official Addgene TikTok at the end of this month! We’ll be sharing...lab, along with some behind-the-scenes peaks of Addgene’s people, culture, and workplace. Rumor has it that...

... plasmid encoding rep and serotype-specific cap genes, and a plasmid encoding adenoviral helper sequences...viral vectors are tested in vitro and in vivo for gene expression and/or function. These data are sometimes...copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease P/MRP 30 kDa subunit...vector genome titers by droplet digital PCR. Human Gene Therapy Methods, 25(2), Article 2. https://doi.org...Titer by a Novel Droplet Digital PCR Method. Human Gene Therapy Methods, 29(2), 96–103. https://doi.org/...Production AAV distributed by Addgene has been produced either in-house by Addgene scientists or through collaboration...either performed by Addgene or by the University of Pennsylvania Vector Core. In general, titering is performed...

...plasmid to ensure our assembly result is accurate The gene/insert or any major feature highlighted by the deposited...sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process Addgene prioritizes...deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature” library...Quality Control Now at Addgene Bioinformatics at Addgene Resources on Addgene.org Educational Resources... Figure 2. Addgene’s basic QC process. Created with BioRender.com. Once Addgene receives deposited...receiving the replacement plasmid at Addgene. QC is a team effort Addgene’s QC team is full of dedicated scientists... Resources Additional resources on the Addgene blog Addgene's Tips for Plasmid Quality Control Plasmids...

...mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help scientists...times easier. We’ve certainly felt this way at Addgene so we’ve been putting some work into finding robots... many more robots than you ever knew existed! Addgene recently started automating a few protocols to ... hour. Automating vial fiIling The majority of Addgene plasmids are shipped as agar stabs in glass vials... easily programmed for a variety of lab needs. Addgene uses it to fill as many vials as it can in the ...Label your tubes with a label maker. Label makers generate far more legible labels than your hand and help...avoid sore fingers. There are other process that Addgene would love to move forward and automate. For instance...

...do scroll down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit...improving the tools you use to share and access them. Addgene’s product, development, and content teams have made... made some changes to the look and feel of addgene.org. Some you’ll notice right away, while others may...resources, tools, collections in our catalog, and the Addgene Help Center. A big part of this redesign was pulling... place, so you can quickly navigate around the Addgene website without scrolling up and down the page....skim through. Users with disabilities will find Addgene’s website easier to navigate, thanks to design choices...— check out all the new features and designs addgene.org! And when you’re finished, feel free to take ...

...Halloween is only 2 days away and here at Addgene we couldn’t be more excited! As a small, tight-knit...email system.  Our 2013 winners dressed up as Addgene's favorite lunch... a BonMe noodle meal, crammed...interested in finding out who wins this year’s Addgene costume contest, like us on Facebook and checkout...  addgene...

...these expected hits, we always get hits to “random” genes. Do these hits always mean the sample is contaminated...matters is the number of hits to the same sequence (gene). In a clean sample, there are typically fewer than... However, a large number of hits to one sequence/gene - we set our threshold at >100 hits - immediately...example, AAV-68544 contained 220 hits to the CHRM4 gene. When contamination is suspected, we proceed to...Viral Preparations from Viral DNA Extracts. Human Gene Therapy 31:664–678 . https://doi.org/10.1089/hum...purity. But Addgene is also conducting more rigorous quality control, including next generation sequencing... for transgene expression. Once packaged in AAV, this pre-recombined DNA could lead to transgene expression...

...-invasive systemic gene delivery in non-human primate (NHP) brains.  Systemic gene delivery capsids previously...Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains Endothelial-specific ...Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain....plasmid here! Chen, X., et al. (2023). Functional gene delivery to and across brain vasculature of systemic...past decade, primary cells remain difficult to genetically edit due to toxicity from electroporation or ... under CC-BY license.   The Miyawaki lab generated a series of StayGold mutants and identified a ...mentioning that Kiryl Piatkevich and colleagues generated yet another monomeric StayGold variant (Piatkevich...

...her work has always been focused on gene transfer, from transgenes to plants, chloroplast to the nucleus...easy to express genes in, which makes it a good laboratory workhorse. Tips & tricks Addgene: Do you have ...The MoClo Plant Kit makes it really easy to clone genes for expression in plant cells. Sylvester and I chose... Now there are many, so if you want to make a 10-gene construct, you don’t have to worry about repeating...constructs to allow people to, for example, knock out a gene with simultaneous complementation, with a knockout...Marillonnet share with the scientific community via Addgene, the struggles of plant scientists and how they...Everybody's favorite parts for plant synthetic biology Addgene: Tell me about the Golden Gate Modular Cloning ...