We narrowed to 939 results for: Gene

...to knock-out single genes in clonal populations and to knock-out thousands of genes in mixed population...targeting over 19,000 and 20,000 human and mouse genes respectively, use lentiCRISPR v2 as the backbone...Resources on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read...announce that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...Availability and Use LentiCRISPR v2 is available from Addgene both as a single empty vector and in pooled libraries...reagents available to the research community through Addgene and look forward to another great year of sharing... Plasmids to Mammalian Cells Resources on the Addgene Website Visit our Lentiviral Guide Pages Find ...

... J.A. Engineering the next-generation of CAR T-cells with CRISPR-Cas9 gene editing. Mol Cancer 21, 78 ...dominant cataract disorder caused by a single copy of a gene known as Crygc (Wu et al). They found that the cataracts...able to demonstrate functional correction of the gene in clonally expanded organoids. This work, and many...immunotherapy for cancer treatment by traditional gene therapy pipelines (viral), and researchers are currently...single nucleotide polymorphisms (SNPs) in the TTR gene and is molecularly characterized by misfolding of...blog post on CRISPR’s potential for correcting monogenetic diseases. Now, almost 10 years later, CRISPR’...Resources References Yuxuan Wu et al. "Correction of a Genetic Disease in Mouse Via Use of CRISPR/Cas9." Cell ...

... at Addgene 2. Green, Alexander A., et al. "Toehold switches: de-novo-designed regulators of gene expression...was an in vitro paper-based platform that allowed gene circuits to be deployed out of the laboratory in...combined technologies, we built paper-based synthetic gene networks for the detection of antibiotic resistance...-of-concept work was exciting because it enabled gene circuit-based sensors to be used outside of the ... 3. Pardee, Keith, et al. "Paper-based synthetic gene networks." Cell 159.4 (2014): 940-954. PubMed PMID...Sensor Constructs at Addgene As we report, we have embedded this next generation of toehold switch-based...corresponding portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with...

...permanently knock out or knock-in a gene? Do you want to activate or repress gene expression? Are you trying to...reporter gene (e.g. GFP) to identify and enrich positive cells or a selection marker to generate stable ...conjunction with activator or repressor domains to control gene expression. Base editors can help you make precise... separate transfer vectors. May contain reporter gene (e.g. GFP) to identify and enrich positive cells... for each, and potential delivery methods.   General considerations for planning your CRISPR experiment...Wildtype Cas9 or Cas9 nickase are appropriate for generating knockouts or knock-ins, or introducing mutations...PCR followed by gel electrophoresis and/or next-generation sequencing more appropriate? If you already...

...effectively targets your gene/region of interest is a key step for CRISPR/Cas9 gene editing. Dr. Masahito...sequence or gene of interest into the pCoofy vectors, select the appropriate pair of vector and gene primers...vector and gene primer for SLIC cloning. Amplify the pCoofy vector and the sequence of gene of interest... can be used to generate random mutagenesis libraries or to insert heterologous genes, reporters, or other...Updated mini-transposon vector for bacterial mutagenesis or gene targeting Victor de Lorenzo's lab has...approach to genetic engineering which drastically reduces the time and effort needed to insert new genes into...successfully create GFP fusion proteins with a variety of genes across the genome. Although this tool was published...

...Now that we know all about antibiotic resistance genes, let’s consider another basic element of any plasmid...Do you want to make a lot of your plasmid? Is the gene toxic in high amounts? It is always good to keep...below for additional considerations. *Requires pir gene for replication (reference). **F1 is a phage-derived...copies of plasmids if they contain large inserts or genes that create a toxic product. The E. coli strain...survive within cells. The replicons of plasmids are generally different from the those used to replicate the... to make additional copies. ori sequences are generally high in As and Ts. Why, you ask? Well, A-T base...few copies depending on how they are regulated. Generally, control of replication is referred to as "relaxed...

... extensive heterogeneity in the feature sequences due to genetic drift and the use of genes from different...raw plasmid sequence and display frequently used genes and control elements. Development of this tool required...sequence feature that may be used to make fusion genes, detection needs to occur even if one or two codons...of the feature. With the increasing popularity of gene synthesis, many researchers now use codon-optimized... who uses SnapGene or the free SnapGene Viewer companion product. If you don’t use SnapGene yet, you can...Resources on the Addgene Blog Read about our SnapGene powered plasmid maps Learn how we use SnapGene during the...sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered by SnapGene Browse...

...containing cassettes of the same gene multiple times.  *As of March 2026, Addgene is using long-read sequencing...Resources on the Addgene Blog A Look at Addgene’s QC Process Bioinformatics at Addgene The Power Behind...Previously, we provided a general overview of the QC process at Addgene. All plasmids go through this...technologies that Addgene uses in the QC process, when these technologies are used, and how Addgene keeps up with...Sequencing technologies used at Addgene Sanger sequencing (“First-generation sequencing”)  Sanger sequencing...assembles the raw data.  Addgene’s de novo assembly pipeline usually generates the correct plasmid sequence...revisit the assembly in a program called Geneious. Within Geneious, the raw FASTQ reads are used for either...

...plasmid to ensure our assembly result is accurate The gene/insert or any major feature highlighted by the deposited...sequenced by Addgene’s QC team. Email help@addgene.org to get started! Addgene’s QC process Addgene prioritizes...deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature” library...Quality Control Now at Addgene Bioinformatics at Addgene Resources on Addgene.org Educational Resources... Figure 2. Addgene’s basic QC process. Created with BioRender.com. Once Addgene receives deposited...receiving the replacement plasmid at Addgene. QC is a team effort Addgene’s QC team is full of dedicated scientists... Resources Additional resources on the Addgene blog Addgene's Tips for Plasmid Quality Control Plasmids...

...do scroll down? You’ll find Blugene, as well as information about Addgene, our mission, links to our deposit...improving the tools you use to share and access them. Addgene’s product, development, and content teams have made... made some changes to the look and feel of addgene.org. Some you’ll notice right away, while others may...resources, tools, collections in our catalog, and the Addgene Help Center. A big part of this redesign was pulling... place, so you can quickly navigate around the Addgene website without scrolling up and down the page....skim through. Users with disabilities will find Addgene’s website easier to navigate, thanks to design choices...— check out all the new features and designs addgene.org! And when you’re finished, feel free to take ...