We narrowed to 798 results for: KIN

... There are currently 17 sites around the USA undertaking BEST programs, including local schools in Boston...contributing to the NIH’s Biomedical Research Workforce Working Group. Along with her participation in this panel...Pubmed Central PMCID: PMC3787295. 6. Marder, Eve. "Looking out for future scientists." eLife 3 (2014): e04901...PMC4185419. 7. NIH 2012. Biomedical research workforce working group report. http://acd.od.nih.gov/biomedical_...in Science in Our Career Posts! Get Advice on Networking at Scientific Meetings from Addgene Executive...

...edit and homology to the genomic DNA locus. After nicking by Cas9, the homologous pegRNA sequence hybridizes..., as it can edit near to or far from PAM sites, making it less constrained by PAM availability than other...previously used when they developed base editing. By nicking the non-edited strand, they can cause the cell ...efficiencies 2-3-fold. However, the process of double nicking increases indel formation slightly. Designing the...the edited strand, as in the PE3b system, guides nicking of the unedited strand only after editing has occurred... ✓ ✓ ✓   ✓ Second sgRNA to guide nicking of the unedited strand Favor the edited strand...

...understand the risks of the specific virus you’re working with before you work with it. This will help you...required in BSL-1 and required in BSL-2 labs. When working with lentivirus, many organizations require or ...can quickly be removed and disposed of without risking exposure. You can request disposable gowns from...researchers choose to wear eye protection when they are working in a biosafety cabinet, even if it is not required...used for BSL-2 waste, and of course, if you are working with a substance in a biosafety cabinet, it needs...

...include a lot more. Font size: Making your poster readable While we are talking about font, remember this:...important to reflect on these impressions when thinking about crafting your own poster. What sets a scientific...that it will display your data most prominently, making it the star of your presentation. Portrait. This...presented some standard formatting guidelines for making a scientific poster, you shouldn’t hesitate to ...

...with the NPA where I gained invaluable experience working on and later chairing the Advocacy Committee. I... I’m now furthering my leadership experience working at the Association for Research in Vision and Ophthalmology...website. By volunteering, you can gain experience working with other people mostly online and on monthly ...resulted in actionable tasks and I made a point of sticking to deadlines and performing the work I agreed ...members changed, I had to learn how to develop new working relationships with these individuals who often ...

... months have in store for us. If you’re thinking about making the switch from academia to entrepreneurship...entrepreneurs. To make things more interesting, we are working in a new technical discipline that nobody understands...few jobs available. So, we spent a lot of time talking about what we could do to make ourselves marketable...marketable to the wider world and occasionally thinking about how we could bypass the establishment hiring...

...You’ve spent days and weeks thinking of an amazing project. You’ve written your protocols, designed your...enzymes laying around the lab. If not, you’ll be looking at about $50-$60 per enzyme. Once you’ve got a ... of the restriction enzymes and overall you’re looking at a price of a few dollars per reaction for traditional...relatively new to the cloning game, however, or simply looking for a more streamlined approach, you might want...inserts: There are some cloning methods that make working with multiple inserts a breeze. Among them are ...

...broken down into different types, with Cas3 systems making up type I, the most abundant type in the Class ...consistent expression can still be a challenge. A multitasking Cas Most Cas effectors have one job — Cas see...away at both the non-target and target strands by making cuts every so often. Cutting both strands ensures...carried out much like the name implies — after nicking, Cas3 reels in the non-target strand of DNA much... for Cas3’s biggest trick. Cas3 likes to bluff, making you into think it’s more powerful than it is. The...

... they become confluent, taking both early- and late-arising clones, and making sure we always maintain...densities - adding to the expense of dilution cloning. Taking a page from bacterial cloning, we reasoned that...cloning rings. A picture of a 60 mm dish ready for picking is shown in figure 1.   Find Additional Useful ...firmer 2% agarose gel solution discussed above for picking. Many thanks to our guest blogger Iris Lindberg...

...Additional opportunities: Tracking intracellular pathogens In addition to tracking the location of proteins...University of Queensland) to observe membrane trafficking events in vivo. Locate your protein and see who...your images using subjective judgement by simply “looking at them”. Modern cameras and image analysis software...limited by the wavelength of light and, practically speaking, for common lab microscopes the detection limit...