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  1. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... facet of a project this big. Maintaining our excellent service and accuracy involves caring and careful... it took a lot of work and dedication to do this well ... because it did. So finally, a big thank you ...

  2. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...EvolvR could also be a tool for lineage tracing cells that do not tolerate double-strand DNA breaks by...researchers to trace the progeny of a single starting cell. Additionally, multiplexing of guides allows EvolvR...

  3. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...methylation should be considered when digesting DNA. Why? Well, even though Dam methylation sites are not specifically...later be verified by Sanger sequencing. How can I tell if my enzyme will cut? Whether performing a digest...

  4. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...are trying to block but your primary antibody as well.  Next, Fab and Fab’ fragments are almost interchangeable...fragments for multiplexed super-resolution microscopy. Cell Rep Methods 2(10):100301. https://doi.org/10.1016...

  5. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ... a journal club to my molecular biology class. A Cell paper. With six figures. Averaging ten panels each...strategy that can help. Even something as simple as telling yourself, “I can stop and check my notes if I need...

  6. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...Moreover, avoidance of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence...convince the reviewers that the proposal is strong, well-thought out, and will be successful when funded....

  7. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ... the deposit, NCBI sequence and protein data, as well as available backbone sequences. All of these resources..., with significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary. Resources ...

  8. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...contained all the goldilocks antibodies in one place... well, lucky for you one is in the making by Dr. Zhuhao... Innovative Neurotechnologies (BRAIN) Initiative Cell Census Network, the Wu lab will be screening over...

  10. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...for my interviews in industry. Prepare stories to tell in your interview The STAR interview method is a...prepare stories to answer interview questions. Story telling shares many of the same elements as a STAR answer...

  11. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...developed by the Allen Institute for Artificial Intelligence. Semantic Scholar analyzes publications and ...paper has is displayed at the top of the page, as well as the number of citations highly influenced by ...

  12. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    .... The computer scientists can get involved in modelling, the electronics folks can build a robot worm ...rich and complex microbiome data for analysis. And, well, cats... everyone knows the internet loves cats!...

  13. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...of other people. Some students and postdoctoral fellows are hesitant about expressing their ideas, especially...person that all employers are eager to hire. An excellent resource to learn more about assertiveness skills...

  14. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...different substances can be injected, including cells, drugs, DNA, viral vectors, and viruses. All researchers... injections are most often used when injecting a cell suspension to create xenograft models. Restraint...

  15. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ...year from 2012 to 2023.  What does this tell us? Well, it’s hard to draw any exact conclusions, but...

  16. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...from the PCR process (the precise mechanism isn’t well understood). Finally, TOPO cloning is another good...come with comprehensive and tested protocols, as well as premixed and ready-to-use reagents. Thermo Fisher...

  18. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ...collaborators, or co-workers want to know that you are a well-rounded individual with more going for you than ...! Enjoy that feedback, bask in the glow of a job well done, take some time to think, and write your thoughts...

  19. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ... smaller cargos, and the ability to easily cure cells of the plasmid after integration. Figure ...into the mini-transposon Transform plasmid(s) into cells and plate overnight Screen colonies phenotypically...

  22. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...access, review, and query. Gates Open Research and Wellcome Open Research both accept deposit of data associated...with two of the five: Figshare and Dataverse, as well as the DSpace repository. These integrations utilize...

  23. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    Published
    Oct. 13, 2016, 2:30 p.m.
    ...’ll also talk about the skills you’ll need to do well as a healthcare consultant and what you could do...the challenge at hand and help resolve it through well-informed recommendations. Healthcare consulting ...

  24. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...contributed by guest blogger, Kristian Laursen from Cornell University. Site directed mutagenesis is a highly... Laursen! Kristian Laursen is a researcher at Cornell University.       References 1. Breslauer, Kenneth...

  25. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...notation. Please indicate the amino acid change as well as any functional consequences of that change. For...for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression). This...

  26. 2022 Depositor Week

    Type
    Blog Post
    Published
    Dec. 12, 2022, 2:15 p.m.
    ...Hello and welcome to Depositor Week 2022! It’s that special time of year where we Addgenies use our social...

  28. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...group of talented individuals that makes Addgene — well, Addgene. We're so grateful for each and every one...

  29. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ... to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On the fourth day of CRISPR, ...

  30. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ... of open-source distributors such as Addgene, as well as a number of online user forums such as http:/...
Showing: 721 - 750 of 841 results