We narrowed to 786 results for: KIN

1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   Published

   May 26, 2020, 1:15 p.m.

   ...read-outs of Cas9/gRNA efficiency. So if you are looking to identify the most effective gRNA and enrich ...

2. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

   Type

   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ...adenine base editors can correct all four of these kinds of point mutations, in theory converting the disease-associated...

3. Developing a cold tolerant E. coli using specialized chaperones

   Type

   Blog Post

   Published

   Jan. 28, 2020, 2:35 p.m.

   ...cold-tolerant E. coli! Additionally, we’re also working towards widening the applicability of our strategy...

4. Communicating Your Science With Help From ComSciCon

   Type

   Blog Post

   Published

   July 19, 2018, 1:12 p.m.

   ...milestone in a scientist's career is mediated by some kind of communication to another person.  In an interview...

5. Plasmids 101: Screening Strategies Used in Plasmid Cloning

   Type

   Blog Post

   Published

   Sept. 15, 2020, 1:15 p.m.

   ...perfect clone that contains the plasmid you’re looking for out of the many cells that don’t. One way to...

6. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ...specific transcription response element (TRE) or kinase translocation reporters (KTRs). Find visual barcode...

7. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ...advancement could be especially exciting for scientists working to visualize multiprotein complexes.  Fig...

8. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...purposes or for diagnostics, we suggest double checking to make sure your results will not be affected...

9. Viral Vectors 101: An Introduction to AAV

   Type

   Blog Post

   Published

   May 30, 2023, 1:15 p.m.

   ... experiment! As a starting point, we recommend checking the literature and our AAV data hub to see if ...

10. To Each HIS Own

    Type

    Blog Post

    Published

    March 2, 2023, 2:15 p.m.

    ...1321–1325. Janknecht R, de Martynoff G, Lou J, Hipskind RA, Nordheim A, Stunnenberg HG. Rapid and efficient...

11. CRISPRainbow and Genome Visualization

    Type

    Blog Post

    Published

    Feb. 28, 2017, 3:30 p.m.

    ...rainbow of colors, Ma et al. performed sophisticated tracking of multiple chromosomal loci in live cells. However...

12. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded DNA and...

13. Antibodies 101: Secondary Antibodies

    Type

    Blog Post

    Published

    Dec. 21, 2021, 2:15 p.m.

    ... good deal of it. Researchers are continuously looking for ways to improve direct methods and avoid the...

14. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...some regions of the protein may be masked by crosslinking (ChIP) or fixation (IHC). Because of their increased...

15. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...Göttingen in Germany. I attended this symposium seeking inspiration to resolve the technical hurtle of ...

16. dTAG - You're it!

    Type

    Blog Post

    Published

    June 21, 2018, 2:06 p.m.

    ...BRD2/3/4, CDK9, TRIM24, FLT3, BTK, and ALK) by linking small molecules that bind these target proteins...

17. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    Published

    Oct. 29, 2019, 2:52 p.m.

    ...bioelectrical signals affect pattern memory and decision-making in somatic cell networks. “Developmental bioelectricity...

18. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    Published

    Sept. 24, 2019, 12:55 p.m.

    ...verify that the sequence of the plasmid you are working with is correct. It's also best to use 1-2 ug of...

19. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ... has revolutionized how we decode the genome by making it easy to create specific genetic perturbations...

20. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ... will depend on the particular plasmid you are working with, but, in most cases at Addgene, we check what...

21. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...types, during certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline...

22. A Look at Addgene's QC Process

    Type

    Blog Post

    Published

    May 6, 2025, 1:15 p.m.

    ...ensure the entire team is using the same rubric and making the same decisions when analyzing the wide variety...

23. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...of conjugation, and these are encoded by certain kinds of plasmids — more on that in a minute! Fun fact...

24. Progress Towards a PAM-Free CRISPR

    Type

    Blog Post

    Published

    March 7, 2024, 2:39 p.m.

    ...mean it’s suitable for your experiment. If you’re looking for more pan-genome PAM flexibility, you’ll want...

25. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ...References Lu, Q. L., & Partridge, T. A. (1998). A New Blocking Method for Application of Murine Monoclonal Antibody...

26. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    Published

    March 8, 2017, 3:15 p.m.

    ...process. Figure 1: Begin the deposit process by clicking “Submit Online Deposit”or “Download Deposit Spreadsheet...

27. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type

    Blog Post

    Published

    July 12, 2021, 1:15 p.m.

    ...HY, Tortorici MA, Veesler D, Murphy M, Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents...

28. Overcoming the Challenges of Lentiviral Production

    Type

    Blog Post

    Published

    Aug. 7, 2018, 12:31 p.m.

    ...tips and tricks for overcoming these hurdles. Talking 'bout my (lentiviral) generation The first step...

29. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...Nanoblades? They’re easily produced in a BSL2 lab, making them a versatile and accessible genome editing ...

30. Open Resources and Plasmid Tools For Studying C. elegans

    Type

    Blog Post

    Published

    July 18, 2019, 12:55 p.m.

    ...associate, self-excise, and covalently ligate their flanking peptides together (Wang et al., 2018).” With the...