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Showing: 481 - 500 of 691 results

  2. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...AAV). This issue was recently circumvented by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH...as well as individual neuronal populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific...

  4. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    Published
    April 19, 2018, 1:25 p.m.
    ... had to overcome some hesitation to ‘approach strangers’ and learn how to communicate outside my scientific...message and to keep it short. Soon, discussing with strangers stopped feeling weird and started to be fun. I...

  5. Career Insights: Technical Support Specialist

    Type
    Blog Post
    Published
    Nov. 9, 2017, 2 p.m.
    ...addition, it can be advantageous to speak multiple languages - that makes communication with customers from...requirements (and maybe also the proposed salary range) should help. In smaller companies, technical support...

  6. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...watched each month, in 39 countries, and in over 54 languages. While most scientific videos were for entertainment...researchers for whom English is a second or third language (making publishing their research all the more...

  7. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...and blue triangles. 2) An antibody immobilized on a bead binds specifically to the blue triangle protein...

  8. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...digital PCR (ddPCR) titration protocol (modified from Wang et al., 2018). Briefly, 293T cells are transduced...Article 2. https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination...

  10. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ... of replication (p15A) worked best under a wide range of inducer concentrations (0.1-1% L-arabinose) without...confluent well of HEK cells in a 6-well dish and change the media every day for three days. GVs should ...

  11. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ... been converted to an alanine (D10A or H840A), changing its double-strand cut to a single-stranded nick...for your target gene.  Validated gRNAs are a great bang for your buck – they have been used successfully...

  12. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...supporting datasets must be logically annotated and arranged, as in an actual publication and not require interaction...take interest in how it is released, we will be in danger of having to deliver on well-meaning but impractical...

  13. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...lamps are the best for working in this spectral range. You can also check that no filters and/or cold ...tell us about cellular events via  fluorescence changes occurring due to protein synthesis, degradation...

  14. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...the nuances of the research in as technical of language as possible. Seriously. My suggested litmus test...knowledge that you have given everyone a simple plain language map of where you are going and what you expect...

  15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...for researchers looking to generate long-lasting changes in cells. However, for some applications, integration...construct has the D64V mutation, a single amino acid change (from aspartic acid to valine at position 64) which...

  16. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...techs’ financial books.  To find personnel salary ranges, go to online job information sites (like, indeed.com...focused on research relevant objectives and their tangible resources. For example, if a dean/chair asks “...

  17. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...palette for display (like green-magenta-blue or orange-blue rather than red-green). Even better, show ..., Ferling, I., Gazda, M. A., Hoeppner, L. H., Ilangovan, V., Lo, H., Olson, M., Mohamed, S. Y., Sarabipour...

  18. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...s chromatin structure undergoes significant rearrangement (such as when a stem cell initiates differentiation...researchers to avoid making unnecessary base pair changes as would be the case with ssODNs. One minor drawback...

  19. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...Make one yourself. Instead of thinking “What’s my angle on attacking this problem?”, try thinking “Who can...can I assemble to attack this problem from every angle?” Your science will be better for it. Additional...

  20. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...retroviral vectors because they target a broader range of cells and their integration into the genome is...you put in your culture. As viral production can change from one batch to another. For experimental consistency...
Showing: 481 - 500 of 691 results