We narrowed to 517 results for: ATO

1. Changing Labor Laws Bring Increased Postdoc Wages

   Type

   Blog Post

   Published

   Oct. 25, 2016, 2:30 p.m.

   ...paid. Institutional salary policies are one indicatator of how salaries may changes due to the FLSA. ...

2. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

   Type

   Blog Post

   Published

   Sept. 13, 2018, 1:55 p.m.

   ... screen did fail to identify some known ricin-regulators, but most of these genes are also essential for...

3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

   Type

   Blog Post

   Published

   Sept. 21, 2017, 2:07 p.m.

   ...fluorophore. The fluorescent signal can be used as an indicator to determine whether the target sequence is present...

4. Getting the Most from Your Lentiviral Transduction

   Type

   Blog Post

   Published

   April 7, 2016, 2:30 p.m.

   ...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...

5. Plasmids 101: Control Plasmids

   Type

   Blog Post

   Published

   April 9, 2015, 3:29 p.m.

   ...hypothesis: proper controls! For every experiment, an investigator needs a standard against which the results can...

6. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

   Type

   Blog Post

   Published

   Sept. 1, 2020, 1:15 p.m.

   ...10% expression in primary human T cells and hematopoietic stem and progenitor cells (HSPC)s to ~40% expression...

7. Antibodies 101: Single Chain Fragment Variables (scFvs)

   Type

   Blog Post

   Published

   June 3, 2021, 1:15 p.m.

   ..., and enzymes for cancer, autoimmune, and/or inflammatory therapeutic applications. Antibody fragments...

8. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ... their mask. But while the camera is here, the operator is in a different room.   Design: The previous...

9. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

   Type

   Blog Post

   Published

   April 9, 2019, 1:13 p.m.

   ...et al. "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent...

10. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type

    Blog Post

    Published

    July 10, 2018, 1:08 p.m.

    ...lab (user, date, time, action) are required for regulatory purposes in many labs. Besides this being of ...

11. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type

    Blog Post

    Published

    Feb. 22, 2017, 3:30 p.m.

    ... combine pairs of mutations together to test combinatory effects. One-pot saturation mutagenesis The ...

12. Hot Plasmids: Summer 2024

    Type

    Blog Post

    Published

    Aug. 27, 2024, 1:15 p.m.

    ...dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen total variants...

13. Antibodies 101: Beyond Surface Labeling

    Type

    Blog Post

    Published

    June 27, 2024, 1:15 p.m.

    .... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

14. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type

    Blog Post

    Published

    April 4, 2024, 1:15 p.m.

    .... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity...

15. Site Directed Mutagenesis by PCR

    Type

    Blog Post

    Published

    Aug. 2, 2016, 2:30 p.m.

    ...introduce or remove restriction sites), in mapping of regulatory elements (to mutate promoters/enhancers in reporter...

16. Evolution of Lab Techniques

    Type

    Blog Post

    Published

    June 21, 2016, 2:30 p.m.

    ...DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress...

17. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type

    Blog Post

    Published

    Dec. 2, 2019, 2 p.m.

    ...Spatial Proximity." Journal of Investigative Dermatology 137.12 (2017): e197-e203. PubMed PMID: 29169465...

18. Fluorescent Proteins 101: When GFP lets you down

    Type

    Blog Post

    Published

    Aug. 23, 2018, 12:05 p.m.

    ...25831507 PubMed Central PMCID: PMC4394297. 16. Iwano, Satoshi, et al. "Single-cell bioluminescence imaging of...

19. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type

    Blog Post

    Published

    March 1, 2016, 3:30 p.m.

    ...loop (as might be anticipated to occur within a terminator sequence), since this would directly compete ...

20. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type

    Blog Post

    Published

    May 18, 2018, 7:32 p.m.

    ... tries 5 separate experiments at once to get confirmatory data for a hypothesis and presents only the ...