We narrowed to 904 results for: CAN

...greater than 1.5. Then, using the A260 reading, you can calculate the DNA concentration. Generally, A260 ... their corresponding fluorescence. That way, you can compare the fluorescence of your sample against this...Though not the quickest way to quantify DNA, you can use the agarose gel method to not only find out how... acidic conditions and forms a blue complex that can be quantified at 595 nm. This method is time consuming...the measurements are made in the visible range and can be read with a standard ELISA reader if no other ...sensitivity at low concentrations of DNA and it can’t distinguish between DNA and RNA. See our protocol...This is something that absorbance based methods can’t tell you and you don’t need a spectrophotometer ...

...multiple AAV lots can be run in parallel and provide serotype data in minutes and the scripts  can be easily ... 100 AAV vector preparations and showed that VGS can quickly and reliably confirm the identity of AAV ...present in Cre-dependent vectors and a script that can confirm the serotype of AAV preparations.  Using ...this simplified sequencing and analysis approach can be easily incorporated into standard QC regimens ...

.... The lab demonstrated that XMAS-TREE can be multiplexed and can be used in human pluripotent stem cells...MYC-ChIP-seq data from several MYC-dependent cancer cell lines. It can be applied for identification of essential...  After identification of ABE activity, XMAS-TREE can also be used to purify these modifications and work...with one sgRNA per operon or gene. The sgRNA pool can also target core operons in other pneumococcal strains...

...courses get plenty of theory in their lectures, but it can be difficult to determine how much information they...it in an interactive setting. The problem is…how can you make a “hands-on” lesson on genetic manipulation...let your students play. As your students play, you can observe, answer questions, and guide them to productive...! Accessing and modifying BUILD YOUR PLASMID You can find rules, suggestions, and more details on how ...teaching developmental biology Learn how BioBuilder can help teach students about Synthetic Biology Find ... various diseases. That’s when I thought, if it cannot be hands-on, it will be “brains-on”! I know that...

...literature. I think that delivering constructive critique can help your colleagues at the receiving end, if not...open science in your peer-review Additionally, you can ask that data and resources described in the manuscript...journal you are reviewing for. The code and the data can be deposited in the public on-line repository (like...resources, like constructs or biological material, can be deposited in the specific repositories (Addgene... authors.” By addressing the comments to “you” I can easily check if I would have formulated specific ...

...AAV we have released in the last few months. You can also browse our entire AAV inventory. Our new AAVs...size 20 µL samples of control AAV that scientists can use for serotype testing. These samples are available...non-human primates, where fusion fluorescent proteins can affect DREADD trafficking and localization. The Sternson...encode engineered ligand-gated ion channels that can be activated with FDA-approved drugs. See our inventory...inventory. Flp is another useful recombinase that can be used in combination with Cre for intersectional...

...Crystal violet (CV) is water soluble and can enter the peptidoglycan layer of both bacteria types. In water...on the structure of a bacteria species and how it can be used in a lab? Here we’ll be talking about the...bacteria are stained red or pink. Purpose Gram staining can be used in medical settings to determine the general...bacteria present in an infection. This information can then be used to determine effective courses of treatment...plasmid to be inserted into a specific bacterium, it can be important to consider the type of bacteria it ...bacteria, or monoderms, have a single thick peptidoglycan membrane. Common gram positive bacteria include...negative bacteria, or diderms, have a thinner peptidoglycan membrane and an outer lipid membrane. Common...

...but a longer term and more substantial experience can be achieved by volunteering for a professional organization...Hopefully sharing my experience will show you how you can get started on your own development as a leader. ...become Chair of a Committee overnight (however this can be done if you already have some leadership experience...sections of the NPA’s website. By volunteering, you can gain experience working with other people mostly ...gaining transferable skills such as leadership skills can only assist you and make you more qualified for future.... Becoming involved is not too difficult and you can find opportunities around you. You just need to be...

...make a pretty good team. We have confidence that we can take on any challenge and quickly become experts ...organisms, and GMOs have a bad reputation. The public is can be fearful of this technology and angry at the agriculture...hate GMOs, but what you are doing makes sense. I can support that.” That was interesting. If people could...’s exhilarating to have that sort of freedom. It can also be overwhelming – there is so much knowledge... that the learning curve is pretty steep. But we can rely on one another to celebrate good news or share... pursuing scientific or business enterprises, he can be found climbing mountains or playing with his two...

...Organism box. This small piece of information can significantly reduce your wait time for blastn, blastp, ...sequences (blastn)” program for blastn so that you can extract practically every single reliable base pair...results. This option is not as fast as megablast, but can return longer alignments to compare with your sequencing...mismatches or gaps in the nucleotide sequence, you can run a Translated BLAST (blastx) search to check for...blastx search. An alignment omitting repeated regions can be confusing, such as when you attempt to verify ...reading frame to choose for your sequencing result and can easily translate it, we recommend using blastp over...

...researchers are using. The detailed results of his survey can be found in the full post but TLDR: many users have...school and provides a thorough walkthrough of how you can use PCR to make small sequence changes to plasmid...written in terms any programming noob (like myself) can understand. You’ll also find actionable advice on...emailing (hopefully non-vacationing) scientists. You can find all of our August posts in chronological order...

...Videos can save researchers time by quickly relaying key points about an unfamiliar topic or lab process...detailing Streaking Plates for Single Bacterial Colonies can be found below: Career videos Ever needed career...video, featuring Outreach Scientist Jessica Welch, can be found below: How-to videos Our How-To videos...

...contain miRNA (micro RNA) and other molecules that can be transferred to other cells, inducing epigenetic...them, making it even harder to cure cancer. To understand how cancer cells influence microenvironments ...and vice versa, let’s take a look at cancer cell metabolism. Cancer cells promote oxidative stress leading...comes to breast cancer, Hoshinho and colleagues found that after injecting breast cancer cells into mice...characterization of the tumor microenvironment in breast cancer." Cancer cell 6.1 (2004): 17-32. PubMed PMID: 15261139...and poor clinical outcome in human breast cancers." The American journal of pathology 174.6 (2009): 2023...the Addgene blog Read our cancer related blog posts Learn about our cancer collection pages Read more...

... should have a good mixture of useful tasks that can be accomplished quickly and things that will take...of each day on it. The other half of each day you can spend accomplishing small tasks (like... preparing... easy, but actually doing it in a productive way can be quite difficult. In my experience, planning has... write everything in a Word or google doc that I can comment and make notes on as I progress. I’ve also... their to-do lists at the end of the day so they can jump straight into tasks the next day. If you work...prioritizing and planning in the world, procrastination can easily prevent you from making progress on a task...procrastination recommends testing out how much time you can possibly handle doing a particularly annoying task...

... sheer scale of a large conference can be intimidating. And it can be exacerbated when everyone seems ...different evolutionary lineages share genes that can improve chances of survival for an individual, and...scientists. The connection between mentors and Singletons can accelerate the networking timetable for the Singletons...groups. Through such mentoring programs, scientists can build and expand their networks and further their...career development, and the scientific community can improve the diversity and culture of science. We ...Binning Singletons to be another tool conferences can use to foster a culture that brings more value to...the Society for Advancement of Chicanos/Hispanics and Native Americans in Science’s (SACNAS) annual conference...

...will know just how tricky these tools can be to use. AAVs, which can target different cell types depending...vectors from the Addgene repository can save researchers significant time and removes a variable from the... challenging. Their efficacy in any given system can be affected by any number of factors, including the...the sharing of that exact data. Here, requestors can upload their experimental data in a standardized ...systems. Browsing the Data Hub before selecting an AAV can help a researcher make a more informed choice on ...continue to do so, in the hopes that these findings can help others as much as they've helped us.  Interested...trends in our own repository data, giving us significant insight into which vectors are likely to be of...

...virus and help determine the types of cells that it can infect. The VSV-G envelope is widely used and requested...types of cells. This means researchers who use VSV-G can deliver their genes of interest to a variety of cells...producing our ready-to-use lentivirus preps. You can find our lentivirus production protocol on our website...

...genetic drift, scientists can freeze hybridoma cells before culturing or they can clone the genes from the...When you are originally selecting a hybridoma, you can choose one that produces high-affinity/high-avidity...time as the hybridoma cells divide. These changes can alter the antibody that is actually produced by the...antibodies produced by the hybridoma. Any genetic drive can be identified by sequencing the heavy and light chains...myeloma cells and antibody-producing B cells that cannot replicate, meaning that only hybridomas will survive...you’ll get a false negative signal - the antibody can’t recognize the protein even though it is present ...

...above can be made for expressing your favorite T-cell receptor, or perhaps any other tool that can benefit...articles. Think we missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral...different epitopes on GFP Monoclonal antibodies can yield relatively low signal in immunoassays due to... Fixation, chemical staining, and light exposure can negatively affect FPs during these processes. The...with 90-99% knockdown efficiency across the 11 candidate transcript targets analyzed. CRISPR-Csm presents...

...work will have in the world is the value that you can succeed in communicating. Even the most rigorous,...measured should change established theory. So, what can you do to ensure that your skills in science communication...great science communication comes with practice. You can bootstrap your science communication skills with ...attention and support to work in their field. It can be hugely beneficial to pose these why questions ...workshop.  Our 2018 workshop took place in Boston - you can revisit the activities of our attendees there online...novel scientific research will be wasted if you cannot convince others that it is important and relevant...in science communication from across the US and Canada convene at ComSciCon's annual flagship workshop...