We narrowed to 610 results for: CaS;
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TypeBlog PostPublishedApril 28, 2020, 1:14 p.m....Therapeutics and is an Addgene depositor. KSQ uses a CRISPR-Cas9 platform to systematically test the function of ...
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Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog PostPublishedJan. 24, 2019, 2:24 p.m....transgene. When multiple copies of the transgene cassette are introduced, stochasticity will result in a... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....others, such as AAV2, low yields are the norm. In the case of AAV2 this is thought to be due to the viral particle... -
Plasmids 101: TOPO Cloning
TypeBlog PostPublishedOct. 27, 2016, 2:30 p.m....topoisomerase site inserted into a beta-galactosidase cassette allowing a researcher to perform blue-white screening... -
Plasmids 101: A Brief History of Plasmids and an Improved eBook!
TypeBlog PostPublishedOct. 29, 2015, 2:30 p.m....to integrate into the E. coli chromosome in some cases. This terminology held until the 1960s when scientists... -
Advice for Moving Into Sales After Your Science Postdoc
TypeBlog PostPublishedAug. 21, 2014, 3:15 p.m....apply themselves immediately to the position. In case you are interested yourself, sales and marketing... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog PostPublishedMarch 6, 2014, 7:12 p.m....after that will lead to learning. Listen to our podcast interview with Harvard Medical School researcher... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....dynamics, but they come with a few caveats. In both cases, creating a reporter mRNA requires inserting multiple... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....this “pre-prep” normalization, it is also often the case that individual libraries prepared from multiple... -
FPbase: A new community-editable fluorescent protein database
TypeBlog PostPublishedMay 16, 2018, 1 p.m....after submission. Using FPbase The most obvious use case for FPbase is finding information on a particular... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m....Recombinant AAV vector with ITRs that flank the gene cassette, which contains a promoter, the gene of interest... -
Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond
TypeBlog PostPublishedJune 1, 2021, 1:15 p.m....collaboration between two research groups. In other cases, the collaborations were simpler and the volunteers... -
Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog PostPublishedJuly 21, 2020, 1:15 p.m....on the next step of your experiment. But in many cases, you won’t see any signs of DNA in your final tube... -
Virtual Science Conference Coming Up? Three Addgenies Share Their Experience
TypeBlog PostPublishedJune 30, 2020, 1:15 p.m....workshop. “We made sure that we had a backup plan in case someone’s internet cut out. “ On networking Networking... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....something like puzzling or crafts, while listening to podcasts made for kids. Plan out meals and snacks ahead... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m..... doi:10.1038/nclimate1547 Kunda, Z. (1990). The case for motivated reasoning. Psychological bulletin,... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog PostPublishedSept. 28, 2016, 2:35 p.m....scientific method cannot address. Listen to Our Podcast Interview with Stem Cell Researcher Darrell Kotton... -
Management for Scientists: Delegating is Key
TypeBlog PostPublishedFeb. 20, 2015, 8:30 p.m....process is completed more efficiently. Listen to our podcast interview with Harvard Medical School researcher... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog PostPublishedOct. 9, 2014, 3 p.m....ones), they can be a good starting point. In many cases it is sufficient to use a spectrum for a closely... -
Advice for Scientists Starting a Lab
TypeBlog PostPublishedApril 29, 2014, 1:43 p.m....“off flying his plane to exotic places while occasionally emailing us his worldly advice”, the other was...