We narrowed to 659 results for: Clu

1. What Do I Do Now? Academic v. Non-Academic Career Decisions

   Type

   Blog Post

   Published

   March 9, 2017, 3:40 p.m.

   ...  Non-academia encompasses millions of choices including pharma, tech transfer, management consulting, ...

2. 10 Great Guest Posts We're Thankful For

   Type

   Blog Post

   Published

   Nov. 18, 2016, 3 p.m.

   ...interest. Potential applications of the system include activation and repression of your target gene, ...

3. Viral Vectors 101: Parts of the AAV Transfer Plasmid

   Type

   Blog Post

   Published

   Aug. 11, 2020, 1:15 p.m.

   ...long as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins...

4. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

   Type

   Blog Post

   Published

   June 2, 2020, 1:15 p.m.

   ... can be used in many cell types and organisms including Drosophila, fish, and mice, by replacing the effector...

5. Celebrate Open Data Day with Addgene’s AAV Data Hub!

   Type

   Blog Post

   Published

   March 5, 2020, 2:15 p.m.

   ... with a caption. For microscopy images, please include channel imaged, a color legend for probes/dyes/...

6. Summer SciComm: Science Storytelling

   Type

   Blog Post

   Published

   Sept. 16, 2022, 1:15 p.m.

   ...progress without it… These details often aren’t included in scientific communications, but they’re firmly...

7. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

   Type

   Blog Post

   Published

   June 22, 2021, 1:15 p.m.

   ...Pfizer vaccine could neutralize many variants, including the B.1.1.7 (Alpha variant), B1.1.298, and B.1.429...

8. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

   Type

   Blog Post

   Published

   Sept. 21, 2023, 1:15 p.m.

   ...secondary is not binding endogenous IgGs, you should include no primary antibody controls in your experiments...

9. Antibodies 101: Epitope Tags

   Type

   Blog Post

   Published

   May 11, 2023, 1:15 p.m.

   ...can find FLAG antibodies on Addgene’s catalog including a mouse monoclonal (M2) as well as a chimera (...

10. What the HEK?

    Type

    Blog Post

    Published

    April 18, 2023, 1:15 p.m.

    ... option! Especially if your research interests include receptor biology, ion channels, or involve human...

11. Plasmids 101: TOPO Cloning

    Type

    Blog Post

    Published

    Oct. 27, 2016, 2:30 p.m.

    ...need that free hydroxyl group! You may want to include extra extension time after the last cycle of PCR...

12. Plasmid Cloning by PCR

    Type

    Blog Post

    Published

    March 29, 2016, 2:30 p.m.

    ...similarly, taking the final 18 bases of the ORF, including the stop codon (5'-TGGCATATCTCGAAGTACTGA-3'), ...

13. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type

    Blog Post

    Published

    Sept. 15, 2015, 2:30 p.m.

    ...attacks can be brought on by a number of stressors, including emotional stress or changes in temperature.  “...

14. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type

    Blog Post

    Published

    July 7, 2015, 3:36 p.m.

    ...used to make many other genome modifications, including targeted mutations. SEC-based systems may prove...

15. Hot Plasmids: FRET-Based Biosensors

    Type

    Blog Post

    Published

    May 6, 2014, 1:07 p.m.

    ...said he hopes others will find his tools useful, including his zinc sensor and his genetically encoded magnesium...

16. New and Upcoming Viral Vectors - September 2019

    Type

    Blog Post

    Published

    Sept. 12, 2019, 1 p.m.

    ... browse our entire AAV inventory. Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium...

17. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type

    Blog Post

    Published

    Sept. 3, 2019, 1:15 p.m.

    ...different publicly available metagenomic data including the Tara Oceans Project datasets. This study demonstrates...

18. Improving Transduction Efficiency with Exosome AAVs

    Type

    Blog Post

    Published

    March 14, 2019, 1:54 p.m.

    ... normalized to their genomic titers). Examples include the use of exo-AAV1 for transducing sensory cells...

19. The Importance of High Titer for AAV Transductions

    Type

    Blog Post

    Published

    March 1, 2018, 2:11 p.m.

    ...to be difficult to produce in high titer. These include AAV2, which is known to adhere to the cell interior...

20. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type

    Blog Post

    Published

    June 20, 2017, 2:30 p.m.

    ...Basically, the CO2 goes in and the CO2 goes out. Conclusions and handling recommendations Not all O-rings ...