We narrowed to 700 results for: EED

...recruitment of katanin to that anchor. So, you’ll need to express both the EB3N-VVDfast-iLID anchor module...many tools available for controlling cell dynamics. Need a refresher on VVDfast, iLID, LOV, CRY2-CIB, and...

...their biotinylation. The advantage of APEX is its speed, labeling neighboring proteins in minutes rather...developed in the Ting Lab in 2018 to increase the speed of proximity labeling by promiscuous biotin ligases...

...In the mVenus-APT1, a lipidation motif, which is needed for Golgi localization, is occluded and therefore...circularly permuted yellow fluorescent proteins." Proceedings of the National Academy of Sciences 101.29 (2004...

...Lentiviral vectors can be produced easily, without the need for time-consuming purification processes, and are...delivered to cells, the viral life cycle initially proceeds as usual. Reverse transcription of the transgene...

... A,T or C – but avoid NAG) or guide RNA target (“seed”) sequence. The idea was that these “blocking mutations...locus you are editing, and if the blocking mutation needs to be silent. In our view, it’s always best to change...

... antibody specificity and antigen structure. Proceedings of the National Academy of Sciences 76:3116–3120...nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences 76:4350–4354...

...between cells can rescue aerobic respiration. Proceedings of the National Academy of Sciences USA, 103(... system (Figure 6). This approach eliminates the need for expensive reagents and equipment like incubators...

... expression levels of your gene of interest, as needed. Controlling the expression levels of your gene...approach is that, since there is no selection, you will need to screen somewhere between one hundred and one ...

... avoid overlap with this optogenetic tool, they needed to create a calcium sensor with the following characteristics...could not be used as the excitation wavelengths needed would also activate the optogenetic accuators. ...

...and R. J. Samulski. "Delivering transgenic DNA exceeding the carrying capacity of AAV vectors." Gene Therapy...induces reanimation from general anesthesia." Proceedings of the National Academy of Sciences 113.45 (2016...

...protein of interest in a single cell? Or perhaps, you need to analyze a specific subset of cells in a complex... being used together in an experiment, you would need a way to determine the relative contribution of ...

...foreign DNA with unique properties. This led to a need to develop a classification system for the array... tools using a Cascade complex is tricky, as you need to ensure every unit of the complex expresses at...

...reconstituting, choosing your colors depending on the needs of your experiment (Figure 3). And using self-labeling...TMP-tag or tetracysteine labeling dyes.  If reaction speed is a priority, SNAP-Tag or HaloTag have been shown...

...efficiency isn’t everything — prime editing typically needs to be optimized for each application, and the efficacy...efficiency, although both enzymes still approach or exceed PEmax editing efficiencies for short, simple edits...

...fast protocol, with only 5 hours incubation time needed for a single round of editing, and a subsequent...another species of Enterobacteriaceae, without the need for modification. This finding suggests that the...

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...post, we talked about Addgene’s data processing needs, our criteria for selecting a new workflow management...

... participants will be invited to compete in our speed round data presentation, with two winner each receiving...

...That will warm up the entire lab (hint: you might need some mini marshmallows…). We’ll choose 4 submissions...

...careers in the lab and even those who might just need a refresher on some best practices for introductory...