We narrowed to 738 results for: HA
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TypeBlog PostPublishedJuly 1, 2025, 1:15 p.m....sample with a good A260/280 but a low A260/230 likely has some residual guanidine thiocyanate. Phenol is a ...contamination — just be sure to check that your RNase has not expired (or been forgotten). Improving read...
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Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and rAb plasmids...plant, or mammalian cells. Each of these systems has their advantages and disadvantages. Briefly, bacterial... -
Plasmids 101: Codon usage bias
TypeBlog PostPublishedSept. 27, 2018, 1:09 p.m....native organism.” This is possibly a better idea and has been used successfully in the past (Angov et al.,...functionally analyze the protein produced to ensure that it has folded properly. You may be able to avoid getting... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....University of Miami. The beginning of this century has seen some major advances in light microscopy, particularly...unprecedented detail. One of these advances in microscopy has been light sheet fluorescence microscopy (LSFM). ... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog PostPublishedApril 3, 2014, 8:05 p.m....transcript, you will also need to make sure your plasmid has a promoter suited to working in your host organism... but requires SP6 RNA polymerase. SP6 polymerase has a high processivity. When used for in vitro transcription... -
Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service
TypeBlog PostPublishedJuly 28, 2020, 1:15 p.m....transformation? Great news. A subset of our collection has been prepared as part of our new cloning grade DNA... -
Summer Fun at Addgene!
TypeBlog PostPublishedMay 12, 2022, 1:15 p.m.... Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be open to dancing... -
Our New Antibody Mascot is....
TypeBlog PostPublishedFeb. 24, 2022, 2:15 p.m....fact, they’ve been following a set of clues that has led them to their biggest discovery yet: Addgene ... -
Streaking for Single Colonies: The Streak Plate Challenge
TypeBlog PostPublishedMarch 2, 2021, 2:15 p.m....at Missouri University of Science and Technology, has been teaching the microbiology lab every year for... -
New Viral Vectors - Spring 2025
TypeBlog PostPublishedApril 29, 2025, 1:30 p.m....newest viral vector service, AAV Packaged on Request has seen a fantastic response from the research community... -
Deck the Lab 2022!
TypeBlog PostPublishedJan. 17, 2023, 2:15 p.m.... A post shared by Xi Chen (@seleena78) The lab has officially welcomed the Christmas spirit. It's just... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog PostPublishedNov. 12, 2014, 3:54 p.m....multiple sites in cultured human cells. While it has not yet been demonstrated, they say the vectors are... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....technology and rapid improvements in the field, it has recently become possible to introduce DSBs and concomitant...efficiency across different stem cell types. This has facilitated easy generation of clonal knock-in lines...or try out an alternative tag (3xFLAG, Myc, V5, or HA). Tag-specific PCR genotyping can tell if HDR completely... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m....as they pass our standard QC process and the MTA has been approved. The spreadsheet option is recommended...cloned into the vector backbone). If your plasmid has more than one insert, click "Save and Add Another...primers can be used to verify it. If your plasmid has multiple inserts, enter details for each insert individually... -
Celebrating One Million Plasmids Shared!
TypeBlog PostPublishedSept. 25, 2018, 2:39 p.m....takes to make one plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on time... -
Podcast: Interview with Addgene's Founders
TypeBlog PostPublishedApril 13, 2018, 12:51 p.m....where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge, MA and... -
Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More
TypeBlog PostPublishedNov. 15, 2016, 3:30 p.m....Alex is the first MD PhD we've had on the podcast, has worked extensively with CRISPR, and recently became... -
Editor's Choice, October 2016
TypeBlog PostPublishedNov. 4, 2016, 2:30 p.m.... of excitement at Addgene. Our new viral service has begun to pick up steam (we already have over 70 orders... -
Interview with Bart Newland, Addgene’s Newest Board Member
TypeBlog PostPublishedAug. 3, 2021, 1:15 p.m....therapeutics to treat neurodegenerative diseases. He has earned a B.S. in microbiology and subsequently obtained... -
Congratulations, Deck The Lab winners!
TypeBlog PostPublishedJan. 14, 2021, 1:30 p.m.... and microbe cookies! 🤗 🤗 🤗 We hope everyone has a safe and festive Holiday season. 🙌 🥂 🎄 🎉 😷...