... Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be open to dancing...

...fact, they’ve been following a set of clues that has led them to their biggest discovery yet: Addgene ...

...newest viral vector service, AAV Packaged on Request  has seen a fantastic response from the research community...

...                     A post shared by Xi Chen (@seleena78) The lab has officially welcomed the Christmas spirit. It's just...

...at Missouri University of Science and Technology, has been teaching the microbiology lab every year for...

...multiple sites in cultured human cells. While it has not yet been demonstrated, they say the vectors are...

...technology and rapid improvements in the field, it has recently become possible to introduce DSBs and concomitant...efficiency across different stem cell types. This has facilitated easy generation of clonal knock-in lines...or try out an alternative tag (3xFLAG, Myc, V5, or HA). Tag-specific PCR genotyping can tell if HDR completely...

...where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge, MA and...

...Alex is the first MD PhD we've had on the podcast, has worked extensively with CRISPR, and recently became...

... of excitement at Addgene. Our new viral service has begun to pick up steam (we already have over 70 orders...

...therapeutics to treat neurodegenerative diseases. He has earned a B.S. in microbiology and subsequently obtained...

... and microbe cookies! 🤗 🤗 🤗 We hope everyone has a safe and festive Holiday season. 🙌 🥂 🎄 🎉 😷...

...takes to make one plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on time...

...bridge helix. Created with BioRender.com.   Cas9 has two major lobes, called NUC (for nuclease) and REC...gray). Target DNA strand, light gray.   Once it has found a PAM site, Cas9 needs to check for complementarity...orange) and HNH (red) domains, while the REC lobe has been hidden for visibility. A RuvC loop caps the ...

...these adaptive bacterial and archaeal immune systems has led to extreme sequence divergence that requires ...landmark publication in 2013. Since that point, it has become the most engineered Cas enzyme, with adaptations... CRISPR type that exclusively targets RNA. Cas13 has become a natural successor to traditional RNA interference...

...lab to split my cells later. Eating with friends has a somewhat immediate deadline - even the best of ...can be quite difficult. In my experience, planning has 3 components: A mechanism for blocking off time ...

...FLP efficient The first version of FLP discovered has a temperature optimum of 30 °C and is therefore inefficient...evolution led to the identification of FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand...

...instances where it does. 1) When the backbone vector has multiple BbvCI sites. If these sites have opposite...example like this. 2) When your gene of interest has a BbvCI site(s). If so, the nicking mutagenesis protocol...

...properly into the right and left hemisphere. Addgene has several plasmids that contain SHH, you can find them...in nonmuscle systems in the fly. Spaghetti squash has been studied in many contexts within the fly including...

... tyrosine generating a GFP variant (GFPY66) that has a shifted emission spectra. Thus a successful base...luciferase. References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable...