We narrowed to 743 results for: HA

...coming directly from grad school. The transition has been a bit rough for her (3 weeks in and there's ...Scientist team says, “a well-run meeting where everyone has a voice and the group can come to a mutual agreement...

...sample with a good A260/280 but a low A260/230 likely has some residual guanidine thiocyanate. Phenol is a ...contamination — just be sure to check that your RNase has not expired (or been forgotten).    Improving read...

...recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and rAb plasmids...plant, or mammalian cells. Each of these systems has their advantages and disadvantages. Briefly, bacterial...

...requires. Using the (Last name, Year) format also has the benefit of exposing you to relevant researchers...just gotta do it." At the end of the day, someone has to write the article and that someone is going to...

...invention in the late 1990s, Gateway cloning technology has become very popular as a rapid and highly efficient...attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for many popular...

...University of Miami. The beginning of this century has seen some major advances in light microscopy, particularly...unprecedented detail.  One of these advances in microscopy has been light sheet fluorescence microscopy (LSFM). ...

...transcript, you will also need to make sure your plasmid has a promoter suited to working in your host organism... but requires SP6 RNA polymerase. SP6 polymerase has a high processivity. When used for in vitro transcription...

...fact, they’ve been following a set of clues that has led them to their biggest discovery yet: Addgene ...

...at Missouri University of Science and Technology, has been teaching the microbiology lab every year for...

...transformation?  Great news. A subset of our collection has been prepared as part of our new cloning grade DNA...

...newest viral vector service, AAV Packaged on Request  has seen a fantastic response from the research community...

... Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be open to dancing...

...                     A post shared by Xi Chen (@seleena78) The lab has officially welcomed the Christmas spirit. It's just...

...multiple sites in cultured human cells. While it has not yet been demonstrated, they say the vectors are...

...technology and rapid improvements in the field, it has recently become possible to introduce DSBs and concomitant...efficiency across different stem cell types. This has facilitated easy generation of clonal knock-in lines...or try out an alternative tag (3xFLAG, Myc, V5, or HA). Tag-specific PCR genotyping can tell if HDR completely...

...as they pass our standard QC process and the MTA has been approved. The spreadsheet option is recommended...cloned into the vector backbone). If your plasmid has more than one insert, click "Save and Add Another...primers can be used to verify it. If your plasmid has multiple inserts, enter details for each insert individually...

...combining the function of both editors into one? This has been done by fusing the adenine and cytosine editing...deaminases with a Cas9 nickase (Zhang et al., 2020). It has increased CBE activity and reduced RNA off-targeting...engineering and directed evolution of the TadA domain has yielded base editors that are equally efficient at...which uses a different mechanism than base editing, has allowed scientists to replace entire sequences of...

...takes to make one plasmid and how much time Addgene has saved researchers. Assuming 5 hours of hands-on time...

...therapeutics to treat neurodegenerative diseases. He has earned a B.S. in microbiology and subsequently obtained...

...where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge, MA and...