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We narrowed to 878 results for: IND

Showing: 481 - 500 of 878 results

  1. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...chemogenetics in our chemogenetics guide Find viral preps for your research Find chemogenetics plasmids for your...you choose, there is no “perfect” drug that only binds to one receptor, so it is important to fully characterize... ligands. PSAMs themselves are modified ligand binding domains. When coupled to the ion pore domain (IPD...Ligand-Dependent Analgesia, as well as Ligand-Independent Functional Changes in Sensory Neurons. The Journal...

  2. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...expression in your target cell line, researchers often find themselves faced with a roadblock. In this post,...bit more challenging. With these considerations in mind, when planning an experiment users should weigh ...the best for others. For example, in our hands we find that a double harvest approach at 48 and 72h produces...collected from an unconcentrated prep. With this in mind, if the experiment requires a certain volume of ...page Resources on Addgene.org Lentiviral Guide Find lentivirus plasmids Browse all viral vectors ...

  3. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ... plasmid. (Right) An assembled antibody protein binds an antigen. Image from Fvasconcellos.     How...Each antibody would then be tested for antigen binding. If starting from an established hybridoma line...and maturation in vitro by selecting for antigen binding from libraries of recombinant antibodies. The selection... and producing antibodies from plasmids is much kinder to our animal friends! Finally, if we want better... Leonhardt H (2015) Nanobodies and recombinant binders in cell biology. Journal of Cell Biology 209:633...

  4. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...takes about one day, even for six plates (576 individual plasmids)! These libraries are then QC’d, pooled...the raw data from each sample in our pool and individually assembles the reads into a single FASTA sequence...necessarily expecting a perfect match - we will often find a few mismatches in the origin of replication or...insertions that could compromise function. When we do find mutations, we check to see if they affect the translated... detecting common features using Snapgene. If we find information that differs from what we would expect...

  5. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ... used to separately introduce each mutation independently into different mice, and then these strains ...generations as well, a concept known as linkage. This hinders the likelihood that mice in the F2 generation and...may also be either constitutively expressed, or inducible (this is explained more in Part 1 of this series...PMC4448610. Additional resources on the Addgene blog Find our Plasmids 101 blog posts Read blog posts about...engineering blog posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering Guide...

  6. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...popular split-FP plasmids from our catalog to help you find the right split-FP plasmid for your experiment.  ...Browse the table below, and click the Article link to find all the plasmids from the associated paper:   ... a protein fusion may disrupt functionality or binding of your target proteins, especially if the termini... principles and experimental design. We hope you find these plasmids useful. Good luck, and happy experimenting...peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015)  Reference...

  7. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...over 300 Chinese scientists eager to hear current findings in nucleic acid science. I was delighted to see...if the bags contained flora or fauna. I tried to find out what certain ingredients might be used for (...Molecular Medicine presented another unexpected finding in the regulatory RNA field. The Rajewsky lab is...system, his lab has created a tetracycline cas9 inducible knock-out system. His lab is also exploring the...Thomas Steitz of Yale. He gave an update on new findings from structural studies on the ribosome performed...

  8. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...develop new working relationships with these individuals who often had different styles from their predecessors...laid out plan of action, others would act more independently and some others would need regular re-direction...organization speaks in “one voice” whilst among individual members, many opinions may exist. I learnt to...Becoming involved is not too difficult and you can find opportunities around you. You just need to be prepared...Twitter @JulietAdvocSci.        Further Reading Find more Career articles on the Addgene Blog Read Joanne...

  9. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ... I’m not going to lie, AAV transfer plasmids are kind of a pain to work with. These plasmids contain ITRs...digests. I freeze the bacterial pellet for the remainder of the cultures and later maxiprep only the cultures...18265393. Additional resources on the Addgene blog Find out what makes AAV a versatile tool for gene expression...calculator for planning AAV packaging transfections Find tips for a first time CRISPR user Resources on ...and lentivirus Check out Addgene's virus protocols Find AAV for your research ...

  10. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...techniques Resources on Addgene.org Find basic molecular biology protocols Find plasmid cloning protocols Check...upon growth on LB/Cam/X-gal/IPTG agar plates, indicating successful reconstruction of the coding sequence...assembly that would result in a blue phenotype. Indeed, this was seen in all lacI/ lacZ assembly test ... efficiencies and accurate assembly levels were indeed possible, with correct, in-frame assembly proceeding...

  11. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ... suits discussing multimillion dollar contracts behind closed doors. Fig. 1: The mythical land ...get what I want, that means you don’t. In this mindset, one of us wins, the other loses. Although this...examples depend on many specific details about the individuals, each example provides a scenario where both ...to you being remembered as a person who tries to find solutions that benefit everyone. Even if I “lose...positively if they tried to understand my position and to find a creative win-win solution. What does professional...

  12. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ..., Ras proteins have stumped the pharmaceutical industry for years. They have proven very difficult to ... lack areas that are accessible, high-affinity binding targets for potential therapeutic agents. Nevertheless...demonstrated that a number of novel small molecules can bind directly, albeit weakly, to K-Ras and inhibit its... in human cancers, and synthesizing compounds to bind specifically to their mutated residues. For Ras ...Sun, Qi, et al. "Discovery of small molecules that bind to K‐Ras and inhibit Sos‐mediated activation." Angewandte...

  13. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    Published
    April 19, 2016, 2:30 p.m.
    ...Fluorescent Protein Resources Find Other Plasmids that Glow (Luciferase Plasmids) Find Optogenetics Resources...protein. A dimeric fusion protein will have two FP binding domains forming a building block sufficient to ...your studies and greatly lower your chances of finding artifactual results. Many thanks to our guest ...Addgene Blog Read Professor Snapp's Post on mOX FPs Find the Best Fluorescent Protein for Your Needs Get ...

  14. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...available FP emission colors and 10 points to keep in mind when choosing an FP (or two) for your upcoming experiments... used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato...Fluorescence Complementation (BiFC). 8 points to keep in mind when choosing a fluorescent protein Excitation ...considered when labeling proteins that interact. Indeed, FRET is often used to determine if two proteins...isolated from the Unagi eel was shown to mature independently of oxygen, making suitble for use in anaerobic...

  15. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...rely on standard curves in their day-to-day work. Indeed, viral vector production facilities frequently ...diluted in a series ranging from 1:2 to 1:32. Each individual step of the series is consistent at 1:2. When... from 0 to 1 with 1 being a perfect fit and 0 indicating that there is no linear relationship between ...the %CV of the standard curve, one averages the individual %CVs for each point on the curve. ‌The smaller...complex trendlines. If you don’t have access to this kind of software, don’t give up! Rather than plotting...

  16. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...the inspiration behind ExM, how it works, and some of the limitations to keep in mind whether you are ... within ~250 nm of each other. For reference, individual presynaptic terminals, which are jam packed with...the equipment needed for EM is not something you find in the average cell and molecular biology lab, and...their relative positions, allowing you to resolve individual points with a conventional, diffraction-limited...

  17. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...donor get used as a template for repair? Read on to find out! Homology-directed repair and genome engineering... to form ssDNA and uses the ends of the ssDNA to find a repair template. The highest HDR efficiency is...clones by reading up on validating genome edits to find screening strategies for your experiment. Also learn...homology-directed repair choice in response to CRISPR/Cas9 Induced-double strand breaks. Int J Mol Sci., 21(18): 6461...Resources on Addgene.org Browse All CRISPR Plasmids Find Validated gRNAs for Your Next Experiment Check out...

  18. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Andre K, Stein D, Blick G, Greenberg RN, Kinder C, Zolopa A, Binder-Scholl G, Tebas P, June CH, Humeau LM...addition of a chimeric 5’ LTR containing a Tat-independent promoter. Although safer, this system may be ...839-48. PubMed PMID: 26758916. 7. Transfusion independence and HMGA2 activation after gene therapy of human...Our Lentivirus Guide Learn about Virus Biosafety Find Lentiviral Vectors for Your Research  ...

  19. Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus

    Type
    Blog Post
    Published
    March 17, 2016, 2:30 p.m.
    ...Therefore, the roles/functions of individual proteins can be studied independently of other viral proteins simplifying...this would prevent the study of the proteins independently from each other (see Uses of Minigenome System...luciferase can readily be measured to quantify (indirectly) transcription performed by the viral polymerase...easily studied as each component can be examined independently. In addition, experiments can be performed in...

  20. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...calmodulin binding peptide (CBP), a TEV cleavage site (more on that in a moment), and 2 ProtA IgG-binding domains...with folding and stabilization, as can maltose-binding protein (MBP). Overexpression can also lead to ...KRRWKKNFIAVSAANRFKKISSSGAL 4 Affinity and Purification Binding and elution steps use very moderate buffer conditions...proteases without requiring the addition of an independent cleavage recognition site. In fact, SUMO cannot...
Showing: 481 - 500 of 878 results