We narrowed to 893 results for: IND

... to find protocols that require reagents which aren’t broadly or easily available, so you may find yourself...learn more about your protein of interest, you may find the available amino acids a bit…limiting. It may...type, and growth temperature) may need to be individually optimized for each of the three factors: the...trade-offs. This balance can be extremely tricky to find: each of the factors can negatively impact both ...

...can begin to understand how the brain works. It’s kind of like “the head bone’s connected to the neck bone...information or tools, making them useful tracers, but finding a virus that works well for retrograde tracing ...serotype in both mice and guinea pigs (work you can find in AAV Data Hub!) The cortical pathways to the IC...Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service Resources...Resources on Addgene.org Visit Our AAV Guide Page Find Ready-to-use Virus for Your Research Visit Our Lentivirus...

... plasmids can induce gene-specific silencing temporarily, long-term, and even inducibly depending on the...expression cassettes typically contain a promoter (inducible or constitutive), your gene of interest, and often...Screening libraries, large collections of biological independent variables, are becoming increasingly popular ...or maybe first!) viral experiment? Click Here to Find Viral Vector Resources at Addgene! References and...

...straightforward, but plasmid-based SDM has lagged behind. While commercial kits are available for making...GeneArt cloning - but at a much lower cost. Read on to find out how to replace your SDM method with REPLACR ...some of these methods, chances are you’d like to find a better one. Trehan et al. wondered if, instead... in which the phage recombination machinery is induced using L-arabinose. These cells can be made and ...Reference Pages Choose Your Perfect Plasmid Backbone Find Plasmids for Your Research ...

.... ChIP-seq relies on antibodies to recognize and bind transcription factors directly, but that’s a slow-going... transcription factors and figure out where they bind to the genome. We’ve been doing that for years using...actually use this for tagging any protein in all kinds of different assays, not just transcription factors...two-fold: CRISPR/Cas9 offers high efficiency of inducing double strand breaks and more opportunity for ...Professors Mendenhall and Myers for speaking with us! Find the Plasmids: Plasmids for protein tagging with...

...the cellular consequences of repressing or inducing individual transcripts. The activator (CRISPRa) sgRNA...plasmids were not designed with gene activation in mind. “We had not managed to create a system to allow...really confident about working for most genes and individual targeting modules. Those two things, in my opinion...lentiviral CRISPR libraries and keep the following in mind: These libraries are pooled. Most of these screens...

...plasmid gets integrated into the host DNA or is independently replicated as an episome. The four main types... name suggests, these vectors can replicate independently of the yeast chromosome; however, they tend ...These vectors replicate as though they are small independent chromosomes and are thus typically found as a... size and A-T content of the DNA (apparently independent of a known specific sequence) dictate the replication... 5-FOA*. S. pombe  no no *FOA in fission yeast induces mutation in the ura5+ gene in addition to ura4+...

... Resources on Addgene.org Find Empty Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization...reversible intensity changes (Shaner et al, 2008; Bindels et al, 2017) and photoswitching (Kremers et al,...simple mono-exponential decay (Shaner et al, 2008; Bindels et al, 2017) and cannot be described by a single...which far less power is used. For example you might find that you can get useful data from your chosen FP...

...and why should you learn more about it? Read on to find out how ccdB can make your cloning experiments a...gateway/introduction.html demonstrating the principal behind the “swapping out” of ccdB with the investigator...developed specifically with ccdB-containing vectors in mind, any plasmid that contains the F plasmid (F’ strains...Bernard, P., et al. “The F plasmid CcdB protein induces efficient ATP-dependent DNA cleavage by gyrase....Addgene.org Choose a Molecular Cloning Technique Find Plasmid Cloning Protocols Check Out All of Our Protocols...

...irreproducibility. Luckily, there were other academics and like-minded organizations with similar ideas who were also ...bring together talented and motivated groups and individuals with similar ideas to actually do something about...impactful opportunities - There are many brilliant minds out there, but how do you get them to connect? Through... Through networking of course! Thanks to individuals in the group who were well connected, this diverse...that it is driven by academics, with support of industry partners, not the other way around. Sometimes,...

...: Schematic of iGluSnFR3 (left). GluSnFR3 in pMinDisplay (PDGFR anchoring domain), in electroporated in...where she engineers genetically encoded voltage indicators. Tian developed a video-based high-throughput...archaerhodopsin‑derived genetically encoded voltage indicator which had improved performance in live mouse brains... Currently, she is using the improved voltage indicator coming from the screen to understand mouse cortical...Tian. Figure 5: Citrine-tagged voltage indicator expressed in mouse cortex. Image from He Tian....

...bench, we need to (this is a little simplified): Find the appropriate glycerol stock within our freezers...figure it out if they needed to, they’d have to individually keep track of any failures and do troubleshooting... are using barcodes (and you should be!) bear in mind that your barcodes are being applied to the containers...writing software tools to help scientists. You can find him on Twitter @krisshamloo and at krisshamloo.com...Experiments Additional Resources on Addgene.org Find Plasmids for Your Research ...

...expression in your target cell line, researchers often find themselves faced with a roadblock. In this post,...bit more challenging. With these considerations in mind, when planning an experiment users should weigh ...the best for others. For example, in our hands we find that a double harvest approach at 48 and 72h produces...collected from an unconcentrated prep. With this in mind, if the experiment requires a certain volume of ...page Resources on Addgene.org Lentiviral Guide Find lentivirus plasmids Browse all viral vectors ...

... used to separately introduce each mutation independently into different mice, and then these strains ...generations as well, a concept known as linkage. This hinders the likelihood that mice in the F2 generation and...may also be either constitutively expressed, or inducible (this is explained more in Part 1 of this series...PMC4448610. Additional resources on the Addgene blog Find our Plasmids 101 blog posts Read blog posts about...engineering blog posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering Guide...

...popular split-FP plasmids from our catalog to help you find the right split-FP plasmid for your experiment.  ...Browse the table below, and click the Article link to find all the plasmids from the associated paper:   ... a protein fusion may disrupt functionality or binding of your target proteins, especially if the termini... principles and experimental design. We hope you find these plasmids useful. Good luck, and happy experimenting...peroxidase (Alice Ting Lab 2016)  Split-Cas9 for inducible genome editing (Feng Zhang Lab 2015)  Fluorescent...

...chemogenetics in our chemogenetics guide Find viral preps for your research Find chemogenetics plasmids for your...you choose, there is no “perfect” drug that only binds to one receptor, so it is important to fully characterize... ligands. PSAMs themselves are modified ligand binding domains. When coupled to the ion pore domain (IPD...Ligand-Dependent Analgesia, as well as Ligand-Independent Functional Changes in Sensory Neurons. The Journal...

...stronger binding antibodies will require harsher stripping to remove, so start with the weaker binding ones...stripping solution uses low pH to disrupt antibody binding to antigens. Ingredient Concentration or... time will depend on your blot — antibodies that bind more strongly or that have saturated the blot may... the Addgene blog The Basics of Western Blotting Find other tips and tricks for molecular biology protocols...

... plasmid. (Right) An assembled antibody protein binds an antigen. Image from Fvasconcellos.     How...Each antibody would then be tested for antigen binding. If starting from an established hybridoma line...and maturation in vitro by selecting for antigen binding from libraries of recombinant antibodies. The selection... and producing antibodies from plasmids is much kinder to our animal friends! Finally, if we want better... Leonhardt H (2015) Nanobodies and recombinant binders in cell biology. Journal of Cell Biology 209:633...

...takes about one day, even for six plates (576 individual plasmids)! These libraries are then QC’d, pooled...the raw data from each sample in our pool and individually assembles the reads into a single FASTA sequence...necessarily expecting a perfect match - we will often find a few mismatches in the origin of replication or...insertions that could compromise function. When we do find mutations, we check to see if they affect the translated... detecting common features using Snapgene. If we find information that differs from what we would expect...

...over 300 Chinese scientists eager to hear current findings in nucleic acid science. I was delighted to see...if the bags contained flora or fauna. I tried to find out what certain ingredients might be used for (...Molecular Medicine presented another unexpected finding in the regulatory RNA field. The Rajewsky lab is...system, his lab has created a tetracycline cas9 inducible knock-out system. His lab is also exploring the...Thomas Steitz of Yale. He gave an update on new findings from structural studies on the ribosome performed...