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Showing: 481 - 500 of 759 results

  1. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...target sites 5' of the 5'NGG3' PAM sequence as directed by a gRNA. 2A-Puro In this plasmid, SpCas9 is ...stand breaks and may better facilitate homology directed repair. You can find all of these variants, as...

  2. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ..., and almost all universities and institutions require them for material transfers. When Addgene entered...and emails it straight to you, or we can send it directly to your institution’s MTA coordinator. Organizations...

  4. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    Published
    Jan. 17, 2018, 2:57 p.m.
    ...otherwise ubiquitous promoter. You could make an entirely new mouse line with mCherry controlled by a neuron...gene of interest in neurons. This ability to be paired with any Cre-controlled gene of interest makes ...

  5. Editor's Choice, September 2016

    Type
    Blog Post
    Published
    Oct. 7, 2016, 4 p.m.
    ...smartphone apps you can use in the lab. Whether you’re tired of calculating dilutions or you can’t quite remember...contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher at ...

  7. SciComm with the Experts at Science in the News Part 2

    Type
    Blog Post
    Published
    April 27, 2017, 2:30 p.m.
    ...graduate student at Harvard University, former Co-director of Science in the News, and member of the SITN...her PhD from Harvard University, is a former Co-director of Science in the News, and is a member of the...

  8. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...of newspapers. Science is now being adapted to inspire to a new generation. Daniel Stoupin is a PhD scientist...The other thing photography has shown to me - it requires an awful amount of skill,  time for which most...

  9. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...smartphone. Evernote allows you to create notes directly from webpage clippings, photographs, and file ... to the necessary time and resource investment required to change the status quo. But with recent improvements...

  10. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ... been difficult to study without biosensors to directly visualize their activity. In 2019, the first generation..., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & ...

  11. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    Published
    Aug. 11, 2015, 2:30 p.m.
    ...is key: due to differences in the PAM sequences required by each ortholog, a gRNA designed for one dCas9...fluorescently labeled dCas9 isoforms are efficiently directed to the proper target sequence. They were also ...

  13. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...These apurinic sites if in double stranded DNA are repaired by base excision repair, but can lead to mutations...concentration. Alternatively, if the priority is to directly use your DNA preparation in any downstream application...

  14. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ..., and, as mentioned earlier, how frequently the entire group will meet. You and your collaborators should...with everyone involved can speed up the future directions of the project. The state of research is constantly...

  16. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ...Your next cool experiment requires some AAV.  Where do you start? Plasmids of course! You just need three...gene The rep gene encodes four proteins that are required for viral genome replication and packaging: Rep78...

  17. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...widely adopted. So thank you to the camelids who inspired this versatile family of tools, the applications...Antibodies  Secondary Nanobody Toolbox No Llamas Required - Synthetic Nanobodies Against Membrane Proteins...

  18. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...companies have the potential to make a huge impact directly on waste, but also on culture. Encourage your ...posts and updates from Labconscious. If you’re inspired by this post then I challenge you to take to action...
Showing: 481 - 500 of 759 results