We narrowed to 798 results for: KIN

...interest and 50 nucleotides of homology to regions flanking the target site).  The best applications for dsDNA...Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald L. Court. An efficient...Read about Homology Directed Repair Use Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR...

...the template strand. Therefore, I suggest that picking a crRNA complementary to the template strand could....For ssODN design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG PAM to NGC in ...PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift in the...

...the better the detection of the target marker. Looking at Table 1, we can recognize a couple of fluorophores...brightness levels are often not that great. When thinking of terms like "very bright" vs. "dim" you might...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

...Challenge, now taking place Dec. 6, 2022! Share your AAV data, connect with other researchers working with AAV...

...community, we’re asking you for plasmid suggestions. Can’t find a plasmid you’re looking for in the repository...

...’s Day on the Addgene blog.  We’ve been mostly working remotely for the last year to give our lab and ...in the raffle, you will receive an email from us asking for your mailing address and T shirt size. You...

...of Addgene's winter holiday celebrations! We're kicking it off by sharing all the incredible guest posts...NEB's DADExperts: Matthew Norton and NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan...

...I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020...video channels are also underrated and I recommend checking them out, especially since videos on synthetic...

...better way to start off our holiday season than by thanking the twelve guest bloggers and subject matter experts...Toj, a summer intern who shared their experience working at Addgene. An Addgene Summer Internship Matteo...

...crystal structures demonstrating “the structure is strikingly similar to the design model at atomic resolution...That made it a powerful proof of concept that this kind of protein design is possible. Although Top7 was...

... on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral name (and it can’t hurt if...is included in the final four will receive some rockin’ newly designed Addgene swag. Good luck and happy...

...decorate your lab in pipette tip snowflakes, hang stockings filled with tube rack “gifts”, or prepare for ...Here’s an example from last year’s contest:   🎶Walking in a Winter Wonderlab🎶 We decided to #DeckTheLab...

...culture dish and number of dishes used. Here’s to making AAV packaging a little easier! Click here to download...AAV Packaging and Transfer Plasmids Here New to Making AAV? Check out Addgene’s Step-By-Step Protocol ...

...was a year like no other, with many scientists working remotely instead of from the lab. Nonetheless, ...viruses both exhibit similar forms of symmetry. 🎶Walking in a Winter Wonderlab🎶 We decided to #DeckTheLab...

...we mentioned a few months ago, Addgene has been working with Seeding Labs to provide plasmid grants to ...Blog Learn about Plasmid Awards to Researchers Working on Rare Diseases Read About Some of Our Outreach...

..., check out my Recycling and Innovations posts. Making small changes in your daily habits can feel ineffective...wouldn’t leave your oven on all day to save time making dinner at night, so why leave your lab equipment...

...We'd like to thank Dr. Koeris for taking the time to talk to us and taking up a position on the Addgene Board...

...Looking to kick your Zoom background game to the next level? Have you been using Zoom’s default backgrounds...download the image background.     If you’re working with AAV or lentivirus in your research, you might...

...lentiCRISPR v2, you can take a look at the protocol kindly provided by the Zhang lab. Even though lentiCRISPR...around the world. We thank our many depositors for making these reagents available to the research community...

...Their muscles showed an increase in inflammatory cytokines as a result of those mild stressors as usual, ...use a good run right about now. And, if you are looking to get your hands on PGC-1a for studies in your...