We narrowed to 804 results for: KIN

...RNase III enzyme named Dicer (Figure 1). The plant kingdom has expanded its repertoire of Dicer enzymes and...silencing effect through targeted mRNA degradation, blocking translation, or chromatin modifications of complementary...researchers studying plants and other organisms lacking this innate immunity need not worry and have benefited...

... and SciFi.  Scientists around the world began looking for the magic molecule that serves as the code ...insatiable appetite of the moviegoers for dinosaurs wreaking havoc in a theme park.  Released in 1997, GATTACA... at University of California San Diego. She is working in collaboration with Prof. Alexis Komor and together...

... a method relies on accurate ligation of nicks lacking gaps or mismatched base pairs (as in the case for....6 (2004): 757-764. PubMed PMID: 15582400. 2. Tomkinson, Alan E., et al. "DNA ligases: structure, reaction...: 16464020.  3. Ellenberger, Tom, and Alan E. Tomkinson. "Eukaryotic DNA ligases: structural and functional...

... however NLuc’s emission peak is at 460 nm thus making it non-optimal for in vivo imaging. The solution...about to create an in vivo imaging reporter by linking two CyOFP1 molecules to NLuc (CyOFP1-NLuc-CyOFP1...the fluorescent donor for a bioluminescent donor, making BRET probes. Here the bioluminescent donor is unaffected...

...interest and 50 nucleotides of homology to regions flanking the target site).  The best applications for dsDNA...Daiguan Yu, Hilary M. Ellis, E-Chiang Lee, Nancy A. Jenkins, Neal G. Copeland, and Donald L. Court. An efficient...Read about Homology Directed Repair Use Knockout/Knockin Plasmids to Disrupt Genes in Mammalian Cells CRISPR...

...the template strand. Therefore, I suggest that picking a crRNA complementary to the template strand could....For ssODN design, remember to incorporate PAM-blocking mutations (e.g. convert the NGG PAM to NGC in ...PCR genotyping (Sanger sequencing); use primers flanking the tag sequence and expect a size-shift in the...

...the better the detection of the target marker. Looking at Table 1, we can recognize a couple of fluorophores...brightness levels are often not that great. When thinking of terms like "very bright" vs. "dim" you might...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

...Challenge, now taking place Dec. 6, 2022! Share your AAV data, connect with other researchers working with AAV...

...community, we’re asking you for plasmid suggestions. Can’t find a plasmid you’re looking for in the repository...

...’s Day on the Addgene blog.  We’ve been mostly working remotely for the last year to give our lab and ...in the raffle, you will receive an email from us asking for your mailing address and T shirt size. You...

...of Addgene's winter holiday celebrations! We're kicking it off by sharing all the incredible guest posts...NEB's DADExperts: Matthew Norton and NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan...

...I spent about seven years working in research labs, and then pivoted to writing full-time in mid-2020...video channels are also underrated and I recommend checking them out, especially since videos on synthetic...

...better way to start off our holiday season than by thanking the twelve guest bloggers and subject matter experts...Toj, a summer intern who shared their experience working at Addgene. An Addgene Summer Internship Matteo...

...crystal structures demonstrating “the structure is strikingly similar to the design model at atomic resolution...That made it a powerful proof of concept that this kind of protein design is possible. Although Top7 was...

... on Twitter. As with Blugene and Aavery, we’re looking for a gender neutral name (and it can’t hurt if...is included in the final four will receive some rockin’ newly designed Addgene swag. Good luck and happy...

...decorate your lab in pipette tip snowflakes, hang stockings filled with tube rack “gifts”, or prepare for ...Here’s an example from last year’s contest:   🎶Walking in a Winter Wonderlab🎶 We decided to #DeckTheLab...

...culture dish and number of dishes used. Here’s to making AAV packaging a little easier! Click here to download...AAV Packaging and Transfer Plasmids Here New to Making AAV? Check out Addgene’s Step-By-Step Protocol ...

...was a year like no other, with many scientists working remotely instead of from the lab. Nonetheless, ...viruses both exhibit similar forms of symmetry. 🎶Walking in a Winter Wonderlab🎶 We decided to #DeckTheLab...

...we mentioned a few months ago, Addgene has been working with Seeding Labs to provide plasmid grants to ...Blog Learn about Plasmid Awards to Researchers Working on Rare Diseases Read About Some of Our Outreach...

..., check out my Recycling and Innovations posts. Making small changes in your daily habits can feel ineffective...wouldn’t leave your oven on all day to save time making dinner at night, so why leave your lab equipment...