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We narrowed to 720 results for: Met;

Showing: 481 - 500 of 720 results
  1. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...Staining. https://serc.carleton.edu/microbelife/research_methods/microscopy/gramstain.html Smith, A. & Hussey...medicine and more successful experiments. It’s something that all scientists should understand when stepping...
  2. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    Published
    April 7, 2022, 1:15 p.m.
    ...visualizing glutamate neurotransmission.” Nature Methods (2013) PubMed PMID: 23314171. PubMed Central PMCID...variants of the glutamate sensor iGluSnFR.” Nature Methods (2018) PubMed PMID: 30377363 Aggarwal, Abhi, et...
  3. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...vesicles or microvesicles (approx. 40 - 150 nm diameter) that are secreted from the cellular membrane ...Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development 9 (2018): 278-287. PubMed...
  4. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... & Molecular Biology, and Biochemical Research Methods. We also pulled the big interdisciplinary journals...represent different research areas, target readers, methodologies, or qualities of research our adjustment controls...
  5. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    Published
    April 19, 2017, 3:25 p.m.
    ...sequencing have prevented laboratories from using these methods for routine processes like plasmid validation. ... problematic for conventional NGS library prep methods because the yield or quality of individual libraries...
  6. Science Career Options

    Type
    Blog Post
    Published
    Feb. 23, 2017, 1:30 p.m.
    ...Sales Epidemiologist Scientific Recruitment Meteorologist Research and Development Healthcare Consulting...Lab Manager Patent Lawyer Technical Writer Meteorologist Journal Editor Textile Technologist/Designer...
  7. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...addressing these important questions that the scientific method cannot address. Listen to Our Podcast Interview...say that I'm glad we're so fast and affordable. Something in their world should be fast and affordable. ...
  8. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ... will too. Even if you are meeting to discuss something difficult, be excited about the prospect of working...meetings short, informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When ...
  9. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...assignment that was open ended... it was actually something bordering on research, rather than 'here’s the..., sharing ethic that Addgene has behind it is something that very much resonates with me.” About Addgene...
  10. Advice for Scientists Starting a Lab

    Type
    Blog Post
    Published
    April 29, 2014, 1:43 p.m.
    ...lab? Obviously, funding and support are crucial parameters, and familiarity with equipment always helps.... and dealing with email can be half of my day sometimes.” He points out that the copious amounts of email...
  11. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...cells/organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer plasmid...yield dsDNA. Unlike LTRs, ITRs are perfectly symmetrical to allow for this pairing to occur. Like LTRs...
  12. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...another factor, the receptor. The receptor could be something like an antibody, enzyme, DNA, etc. The receptors...One common biosensor class is designed to monitor metal ions. Within these ions, calcium is by far one of...
  13. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...deliver these systems virally instead of by another method? Viral delivery is attractive due to the ease of...viral vectors instead of a traditional transfection method. This is because viral systems have a high infection...
  14. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...with other scientists who maybe hadn’t used this method. I enjoyed writing the first post so I signed up... and use of Twitter and LinkedIn probably had something to do with it. Five resources to help you get...
  15. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...Chemogenetics Chemogenetics offers noninvasive methods for manipulating cell activity, particularly in...targeting. While promoters and AAV serotypes can sometimes be used to target tissue types, recombinases allow...
  16. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...conjugation reaction is done, through a variety of methods or commercial kits.  However, antibodies conjugated...January 31, 2022, from https://www.bu.edu/flow-cytometry/files/2013/06/Fluorochromes-Brightness-Chart.pdf...
  17. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    Published
    June 29, 2021, 1:15 p.m.
    ...cell (National Research Council (US) Committee on Methods of Producing Monoclonal Antibodies, 1999). This...jbc.m116.736942  National Research Council (US) Committee on Methods of Producing Monoclonal Antibodies. Monoclonal ...
  18. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...experts, watch webinars, or use other research methods. In the case of the antibody guide, primary research... as well as consults with microscopy and flow cytometry experts for the panel recommendations. A few discussions...
  19. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type
    Blog Post
    Published
    June 2, 2016, 2:30 p.m.
    ...Alternatively, you can simply make a note in your methods section about the edit that was temporarily used...a lot of space and don’t tell you why you did something or what the result was, so try not to do this....
  20. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ... from a polycistronic transcript. Although this method shortens construct size, it requires coexpression... be processed, a distinct advantage over other methods that favor certain nucleotides at the 5’ end. ...
Showing: 481 - 500 of 720 results