We narrowed to 802 results for: OMP

...of science, even while I was a bench scientist completing my graduate studies in molecular biology and ...for the past three years. pgEd is a small team comprised of individuals from diverse backgrounds and disciplines...social context. This “general public” audience comprises the taxpayers who fund much of scientific research...

...itself. Among other things, science communication comprises broadcasting, science writing, and certain aspects... get on stage for a Science Slam – a sort of competitive TED talk – to win the audience’s applause with...photography and participate in a science photo competition. Or nominate yourself as an educator for a short...

...structure of the hairpin-DNA complex is thought to destabilize the transcription complex, initiating cleavage ...mechanism, whereas Polymerase II termination is more complex and involves two RNA polymerase-associated proteins...

...who are usually antagonistic and impolite, Complainers, who spend most of their time moaning about their...with negative comments (hostile-aggressives, complainers, know-it all experts, wet-blanket negativists...support from your colleagues or other supervisors to complete your work. Whether your supervisor is a negative...

... fluorescent signal throughout the reaction is compared to a standard curve of amplified DNA of a known...The cycle in which the unknown DNA is detected compared to the standard curve can be used to determine... to note however, that multiplex PCR is more complicated to develop and is often less sensitive than PCRs...

...proteins on the cell surface. Figure 1: Comparison of common antibody, HCab, and nanobody. For more...starting library size (up to 1012 members) in comparison to other display systems using phage and yeast...the end of the mRNA molecule, forming a ribosome complex consisting of the translated protein (sybody), ...

... strategy uses new combinations of PE protein components (Figure 1), an altered epegRNA delivered with...protein-coupled receptors (GPCRs) are critical components of many physiological pathways and represent ...et al., 2024). Each plasmid expresses all the components of the biosensor in optimal proportions from ...

...screens have gotten larger, easier, and more comprehensive thanks to the consistent development and improvement...vectors is how the cargo — in this case, the CRISPR components — are expressed. Unlike lentivirus, cargo delivered... away from the comfort of Cas9, and opt for a completely different Cas effector, like Cas12a (Cpf1). Even...

...plants, mammals, and insects. In this post, we will compare two of the most widely used systems, mammalian ... from dedicated adenoviral production labs or companies. Production of both AAV and lentivirus requires...10430340260355347. Vaughn, J. L., Goodwin, R. H., Tompkins, G. J., & McCawley, P. (1977). The establishment...

...RNA (pegRNA). The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension...accounting for some of the inherent inaccuracies of computational prediction models. In addition, we provide options...Switzerland), where he developed a strong passion for computational biology. His expertise includes applying machine...

...the cell lysis process. To ensure that lysis is complete, use a reagent like Qiagen’s LyseBlue, which uses... uses color to show you if lysis is incomplete. Protein expression strains are not good for cloning Bacterial...the material used for inoculation. To further complicate matters, plasmid copy numbers are often ambiguous...

...service free of charge to scientists by allowing companies to pay publish their catalogs on the site. Like...of checking off each step as you painstakingly complete your experiment – one small victory in a long ...manage scientific data: Electronic lab notebooks compared with Google Docs and wikis - by Rory Macneil on...

...also is also called Bimolecular Fluorescence Complementation (BiFC). 8 points to keep in mind when choosing...divided by 1000. In many cases the brightness is compared to that of EGFP which is set as 1. Some proteins... BD bioscience. ilovegfp – a site with very comprehensive data sheets on many FP variants Plasmids 101...

...your samples, the relationships between cellular components, and the accessibility of your target epitope...work by dehydrating and precipitating cellular components. This method of fixation is good for epitopes...away your target (for example, some cytoskeletal components) and you want to fix and permeabilize in one ...

...No examination of the state of science would be complete without the inclusion of some interesting case...Prashers will find it increasingly difficult to compete (for funding, etc.) with that produced by the confident...the U.S. environment, and a decline in the competitiveness of U.S. science and technology.” This last ...

...engineered DNA-binding molecule such as the CRISPR complex is expressed in cells to be analyzed, the Fujii...another "in vitro" form. In this form, the CRISPR complex, consisting of a recombinant dCas9 protein and ... a genomic locus through a specific gRNA-dCas9 complex. When combined with next-generation sequencing,...

...cells can decrease the number of fluorophores, compromising the photon budget. Likewise, the switch from...labeling chemistry easier and more functional in complex biological environments such as live cells and ...brightness of the resulting conjugate, and the complexity of the system. A particularly desirable property...

...importance of the entire GFP protein, which is almost completely required to maintain fluorescent activity; very...may not apply. Table 1: The specific mutations comprising common fluorophores Fluorescent Protein Mutations...chromophore to form with an indole rather than phenol component (cyan derivatives) Y66H Blue-shifts the wavelength...

...are differentially inactivated by nonspecific complement mechanisms in serum. Sometimes this is due to...sugars end up in the envelope and are targets for complement-based killing by anti-alphaGal antibodies. The... as described above and is inactivated by the complement system in serum (DePolo et al., 2000). Other ...

...the anticodon,  a three nucleotide sequence complementary to the mRNA sequence. The 3’ end of  the tRNA...resources (Athey et al., 2017) and many DNA synthesis companies, such as IDT, Genewiz and GenScript, offer tools...the other while other codons are under expressed compared to others. Image from Hiss et al., 2017. Most...