We narrowed to 610 results for: SCR
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TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....visualizing your data, such as R or Python. Write a manuscript. If you have all the data you need to begin writing...
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Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....site-saturation mutagenesis, and medium-throughput screening, she increased the affinity of the sensor for ... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog PostPublishedMarch 14, 2019, 1:54 p.m....producer cells and purification protocols often describe a process that begins with cell lysis to release... -
What Good Citizenship Can Do for Reproducibility in Science
TypeBlog PostPublishedNov. 7, 2019, 2:18 p.m.... LMB in Cambridge UK and then a postdoc at The Scripps Research Institute in La Jolla, California. She... -
Creating accessible biology activities in schools with BioBits
TypeBlog PostPublishedAug. 2, 2018, 12:56 p.m....produce that green fluorescent protein through transcription and translation. Unfortunately, having live ... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog PostPublishedOct. 19, 2023, 1:15 p.m....region of an ITR with a sequence containing transcription factor binding sites can increase transgene ... -
Visualizing Translation at the Single Molecule Level
TypeBlog PostPublishedAug. 1, 2017, 1:15 p.m....cell cycle protein Emi1, showing that ~80% of transcripts with a long 5’ UTR were translationally silent... -
How Dry Ice Affects Viral Vector Sample pH & How to Avoid It
TypeBlog PostPublishedJune 20, 2017, 2:30 p.m....shipment processes. After reading a publication describing how short term storage on dry ice can shift sample... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....all over the world. The rest of this piece will describe plexWell™ in more detail, and how we are using... -
Optimizing Donor DNA for Enhanced CRISPR Genome Editing
TypeBlog PostPublishedMarch 24, 2016, 2:30 p.m....relatively modest, requiring expressed Cas9 protein, transcribed sgRNA, and optional donor DNA. Chris Richardson... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....points. At Addgene, we like to do this live on the screen using a Google Doc. Action items: Before leaving... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog PostPublishedOct. 9, 2014, 3 p.m....has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer comparing a standard... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....time-intensive, so we recommend a variety of ways to screen and verify your plasmids. Here, we'll cover restriction... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m.... — for example, the stem-loop structure of transcriptional terminators. Another potential issue is sequence... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m....controls like FMO or isotype controls, which we’ll describe a bit later. Single Colors Single color controls... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m..... Physiological factors Unlike the previously described sensors, this class measures either factors as... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....through a seminal paper from Peter Schultz’s lab describing a technique that allowed for intentional incorporation... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m....Researchers around the world don't have to start from scratch every time to pursue their scientific curiosity... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m.... genes and is used for genome-wide inhibition screening. Find the Dolcetto lentiviral prep here Control... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....H2B-GFP. This visual read out allows for easy screening of different gRNAs by simply changing the gRNA...