...via restriction enzyme digestion, and used directly in DNA assembly will still yield scarless assemblies... well-designed fragment overlap sections, while still creating scarless plasmid inserts.  Want to Learn...

...avenues previously unavailable to them. With these fantastic tools it’s now easy to monitor protein expression...loops on either side of an enzyme’s active site and still block enzyme function upon exposure to ultraviolet...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter...

..., B-B’, and C-C’) that give the feature its characteristic T-shape. The arrangement of the B-B’ and C-...elements of AAV and rAAV vectors, modifications can drastically affect gene transfer efficiency and transgene... efficiency. Our practical knowledge of ITRs is still limited, but this area of research is important ...

...enough to engineer it to suit our purposes? These questions can be addressed using R bodies: simple, force-generating... modified to extend at different pH levels. Domesticating R bodies We have shown that all three of these...extends to rip the bacteria apart; however, they still remain attached to the ciliate. It’s thought that...

...understands that no question is “dumb” and that it is better to ask before wasting time. Focus on results...support. The manager must be available to answer questions. If you are perceived as too busy to be approachable...

... not naturally exist in mammals, scientists can still reap the benefits of plasmid-based research using...that you may not want the plasmid depleted, but still want to use transient transfection methods. Since...* Mode of Action** Working Concentation*** Blasticidin bsd  HeLa, NIH3T3, CHO, COS-1, 293HEK Inhibits...

...traditional restriction enzyme cloning uses short sticky ends to insert genes into plasmid backbones, base...generated in several ways. One option, imprecise T4 digestion (no dNTPs needed), allows both insert and vector... are generated in the linearized vector by T4 digestion; then the components are combined and annealed...

...Jennifer Doudna’s lab at the Innovative Genomics Institute. What is CasPEDIA? CasPEDIA is an online, searchable... However, many situations could arise where you still need more information. If you are performing a viral... More resources from the Innovative Genomics Institute      CRISPRpedia IGI’s digital resources in...

...and its potential applications. Challenges of existing mutagenesis methods Several continuous diversification...be used to map protein-protein interactions, investigate non-coding segments of the genome, or engineer...flexibility to address many different types of research questions. Are you ready to start EvolvR-ing your targeted...

...the open science movement is that it provides interesting and unique challenges for intellectual property...that balance is, and will continue to be, an interesting legal and professional challenge.Paratore: The...executive (CHIE). He is a member of both the Internal Institute of Auditors and the Information Systems Audit ...

...control AAV in smaller aliquots of 20ul for serotype testing to help you pick the best AAV for your experiment...GABA-ergic interneurons (83895). See our Serotype Testing AAV inventory for the entire collection! Plasmid...next 6 months, pending quality control. For an estimate on availability date or to be notified when a ...

...promoters, origins, tags, and certain ORFs. Their sophisticated detection algorithm easily and precisely identifies...Sequence” tabs. Primers This tab contains a table listing commonly used primers detected in a given nucleotide...thanks to you (our community of scientists) for suggesting these changes, and the SnapGene and Addgene software...

...an important feature of a fluorophore. The key question is how the brightness is best defined or determined... cells within the dish. This is caused by the stochastic nature of plasmid uptake by cells. To correct...precise determination of brightness in cells, under realistic experimental conditions. By determining the brightness...

...of the biosensors used in research are very sophisticated, but at their core they can all be boiled down...tools to measure real-time cellular reactions to stimuli and as well as changes in cellular dynamics during...you covered with viral resources and safety tips. Still worried? We make virus with some of our most popular...

...began with only a few inventory items offered domestically, but by the end of 2016, we expanded our viral...vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014). It is a dual-vector system... Carolina, Chapel Hill. These can be used to investigate the effects of activating particular signaling...

...recombineering to create insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly ... This method can work well, but poor template digestion can result in a high number of background colonies...fragment In vitro Assembly No No Yes Yes DpnI Digestion Yes Yes No No Purified Enzymes Required No No...

...have something in common. Namely, the scientific question you are interested in and this is a great ice ...relationships. Make a list of experimental problems or questions you are pondering and be ready to talk about them...scientists your own age. Some will be friendly so stick with those. Don’t miss social opportunities, but...

...? Sometimes, the funders of your research may stipulate that your article must be published in an open...scope of the journal you are sending it to. It’s wasting everybody’s time if you don’t. Add a clear cover...get writing! Let us know if you have any other questions about writing and publishing your manuscript, ...

...discussion with resources. One of the most common questions I get is, “What are we going to talk about?” There...internet, but start by downloading my handy list of suggestions.  Get started. Have regular meetings. Experiment...ideas from your own mentoring group or with any questions about mentoring. Read the other posts in the series...

...Anterograde and retrograde tracing take advantage of existing transport pathways in neurons. Anterograde transport...neuroscientists for some time due to their advantageous characteristics for brain research and their ability to introduce...Addgene). The Gradinaru Lab at the California Institute of Technology generated novel AAVs (Chan et al...Samaranch, L., Kells, A.P., Mittermeyer, G., San Sebastian, W., Zhou, S., Beyer, J., Forsayeth, J., and Bankiewicz...