We narrowed to 520 results for: Set

1. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ...Partitions that did not contain any target DNA at the onset of PCR are negative, whereas those that did are ...

2. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ... vectors for use with their favorite selection cassette. They could also switch from FLAG to GFP or a ...

3. Plasmids 101: Yeast Vectors

   Type

   Blog Post

   Published

   Feb. 25, 2014, 7:11 p.m.

   ...markers. The MX series of antibiotic resistance cassettes is most commonly employed, with the KanMX being...

4. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

5. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

6. The Future of Research Symposium Boston 2015

   Type

   Blog Post

   Published

   Oct. 20, 2015, 2:30 p.m.

   ...University School of Medicine and the University of Massachusetts Medical School. Graduate education programs ...

7. Plasmids 101: Cre-lox

   Type

   Blog Post

   Published

   Jan. 13, 2015, 3:47 p.m.

   ...either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene...

8. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   Published

   June 27, 2017, 1:03 p.m.

   ...complex. To help troubleshoot your experimental setup as a potential source of error in suboptimal or ...

9. Viral Vectors 101: Calcium Sensors

   Type

   Blog Post

   Published

   Aug. 10, 2023, 1 p.m.

   ...and is a great FRET sensor option if you have the setup necessary for readout.   We’ve got a live...

10. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type

    Blog Post

    Published

    Aug. 20, 2019, 12:31 p.m.

    ...the home cage and places it into the experimental setup, the mouse becomes stressed. It will likely move...

11. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    Published

    May 15, 2018, 12:43 p.m.

    ...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...

12. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type

    Blog Post

    Published

    Dec. 10, 2020, 1 p.m.

    ...enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle. The earliest explorations...

13. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...and a plasmid that contains the Mobile CRISPRi cassette flanked by left and right Tn7 end sequences. Donor...

14. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    Published

    Oct. 30, 2018, 1:04 p.m.

    ...after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School....

15. Plasmids 101: Protein Expression

    Type

    Blog Post

    Published

    June 7, 2018, 1:17 p.m.

    ...amounts of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein...

16. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type

    Blog Post

    Published

    May 22, 2015, 4:58 p.m.

    ...As we pointed out last year2, delivering a DNA cassette encoding the cas9 gene and single guide RNAs (...

17. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type

    Blog Post

    Published

    Dec. 7, 2020, 2:53 p.m.

    ...to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology potential...

18. An Introduction to Adenovirus

    Type

    Blog Post

    Published

    Oct. 3, 2023, 1:15 p.m.

    ...12727116. Borkenhagen, L. K., Fieldhouse, J. K., Seto, D., & Gray, G. C. (2019). Are adenoviruses zoonotic...

19. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type

    Blog Post

    Published

    Dec. 3, 2019, 11:38 a.m.

    ... Seth Kasowitz...

20. Isolating Genomic Regions of Interest with the CRISPR System

    Type

    Blog Post

    Published

    May 30, 2019, 12:44 p.m.

    ... vast amount of data from a single experimental setup. As we’ve seen for many other CRISPR techniques,...