We narrowed to 512 results for: Set
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TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....either side (often called a “lox-stop-lox” or “LSL” cassette) upstream of a gene of interest will prevent gene...
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Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....the home cage and places it into the experimental setup, the mouse becomes stressed. It will likely move... -
Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools
TypeBlog PostPublishedMay 15, 2018, 12:43 p.m....Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids... -
Viral Vectors 101: Calcium Sensors
TypeBlog PostPublishedAug. 10, 2023, 1 p.m....and is a great FRET sensor option if you have the setup necessary for readout. We’ve got a live... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....complex. To help troubleshoot your experimental setup as a potential source of error in suboptimal or ... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....and a plasmid that contains the Mobile CRISPRi cassette flanked by left and right Tn7 end sequences. Donor... -
Plasmids 101: Protein Expression
TypeBlog PostPublishedJune 7, 2018, 1:17 p.m....amounts of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog PostPublishedDec. 10, 2020, 1 p.m....enough to carry the Cas9 gene and a gRNA expression cassette in one single viral particle. The earliest explorations... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....after completing her postdoctoral training from Massachusetts General Hospital and Harvard Medical School.... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m....As we pointed out last year2, delivering a DNA cassette encoding the cas9 gene and single guide RNAs (... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m....to multi-copy genomic integration of metabolic cassettes (Zhang et al. 2020); however, the technology potential... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m.... vast amount of data from a single experimental setup. As we’ve seen for many other CRISPR techniques,... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....12727116. Borkenhagen, L. K., Fieldhouse, J. K., Seto, D., & Gray, G. C. (2019). Are adenoviruses zoonotic... -
Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!
TypeBlog PostPublishedDec. 3, 2019, 11:38 a.m.... Seth Kasowitz... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....useful in gene therapy applications where a distinct subset of the cellular population needs to be targeted... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....Ibraheim R, Gupta A, Sasaki KE, Wu T, Donohoue PD, Settle AH, Lied AM, McGovern K, Fuller CK, Cameron P, ... -
Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor
TypeBlog PostPublishedJuly 23, 2019, 12:46 p.m....fails to upregulate transduction efficiency in the marmoset brain." Neuroscience letters 665 (2018): 182-188... -
Hot Plasmids: Fall 2024
TypeBlog PostPublishedNov. 21, 2024, 1:30 p.m....This strain is then transformed with the sgRNA cassette on a plasmid expressing mCherry and resistance... -
Fluorescent Proteins 101: Aptamer Fluorophores
TypeBlog PostPublishedApril 11, 2017, 2:30 p.m....RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian cells... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....the technical implementation of your optogenetics setup (viral expression, efficiency of laser light transmission...